ABSTRACT
AIM: To estimate the frequency of fungal sensitization and the incidence of allergic bronchopulmonary aspergillosis (ABPA) in asthmatic patients. SUBJECTS AND METHODS: A total of 140 asthmatic patients were examined. They underwent allergologic (skin tests for fungal allergens, estimation of total and fungal allergen-specific IgE levels) and mycological (microscopy and inoculation of respiratory biosubstrates) examinations. Chest computed tomography, when indicated, was done. A group of patients with ABPA and that of patients with severe asthma and fungal sensitization were identified. RESULTS: The frequency of fungal sensitization in asthmatic patients was 36%; the main allergenic fungi were Aspergillus and Alternaria. The incidence of ABPA was as high as 4% in the patients with asthma and 11% in those with severe asthma and fungal sensitization. CONCLUSION: The given current diagnostic criteria will assist practitioners to identify ABPA, to prevent its progression, and to initiate specific anti-inflammatory and antifungal therapy in due time.
Subject(s)
Alternaria/immunology , Antigens, Fungal/analysis , Aspergillosis, Allergic Bronchopulmonary , Aspergillus/immunology , Asthma , Immunoglobulin E/analysis , Adult , Aspergillosis, Allergic Bronchopulmonary/diagnosis , Aspergillosis, Allergic Bronchopulmonary/epidemiology , Aspergillosis, Allergic Bronchopulmonary/etiology , Aspergillosis, Allergic Bronchopulmonary/immunology , Asthma/complications , Asthma/diagnosis , Asthma/immunology , Female , Humans , Incidence , Male , Mycological Typing Techniques/methods , Prospective Studies , Russia/epidemiology , Skin Tests/methods , Statistics as TopicABSTRACT
The objective of the present paper was to propose an optimal scope of diagnostic studies for the patients complaining of rhinitis symptoms for the early detection of allergic rhinitis, including a differential diagnosis with associated respiratory tract infections. A comprehensive treatment is offered including the use of vasoconstrictors and antihistamines, eliminationand immunocorrectivetherapy with Ribomunyl.
Subject(s)
Antigens, Bacterial/therapeutic use , Immunologic Factors/therapeutic use , Respiratory Tract Infections/diagnosis , Rhinitis, Allergic/diagnosis , Rhinitis, Allergic/drug therapy , Humans , Respiratory Tract Infections/drug therapyABSTRACT
A high-performance capillary electrophoresis (HPCE) method is described for the quantitative determination of the anticancer drug prospidin in human tissue after its derivatization with diethyldithiocarbamic acid sodium salt (DDTC). It was found that absorption of prospidin and its derivatives on the capillary wall due to the strong positive charge in the drug molecules could be eliminated by increasing the methanol content in the run buffer up to 50% and increasing the pH value up to 11.2. While studying the conditions of the interaction between prospidin and DDTC, a molar excess of the latter of 1:9 and 1.5 h of reaction time were found to be enough for complete derivatization. Sample preparation included homogenization of freshly cut papilloma species and deproteinization by methanol addition. Detection was by ultraviolet (UV) absorption at 254 nm. Due to its speed and high performance in separation, HPCE was found to be well suited for the fast checking of drug therapy in clinical practice.
