ABSTRACT
Low-density lipoproteins isolated by a selective precipitation procedure have been investigated in cholesterol-fed rabbits exposed or not to carbon monoxide. The main findings are a higher increase of their cholesterol content and cholesterol to phospholipid molar ratio without a modification in lecithin-cholesterol-acyltransferase activity in intoxicated animals. Thus, the aggravating effect of carbon monoxide exposure on the atherogenic properties of these lipoproteins could accelerate the development of atherosclerosis in cholesterol-fed rabbits.
Subject(s)
Carbon Monoxide Poisoning/blood , Cholesterol/blood , Hypercholesterolemia/blood , Lipoproteins, LDL/blood , Animals , Carbon Monoxide Poisoning/complications , Cholesterol, HDL , Cholesterol, LDL , Hypercholesterolemia/complications , Lipoproteins, HDL/blood , Male , Phosphatidylcholine-Sterol O-Acyltransferase/metabolism , Phospholipids/blood , RabbitsABSTRACT
Serum LDL-cholesterol concentrations were measured after precipitation in various forms of dyslipidaemia. LDL-cholesterol values were increased in hypercholesterolaemia and decreased in hypertriglyceridaemia. The LDL-cholesterol/total cholesterol ratio decreased with the presence of triglycerides. These data have prompted the authors to propose a classification of dyslipidaemia into hyper-and hypoLDLaemia. Such a classification would be helpful in the prevention, diagnosis and therapeutic surveillance of these diseases.
Subject(s)
Cholesterol/blood , Hyperlipoproteinemias/blood , Lipoproteins, LDL/blood , Chemical Precipitation , Cholesterol/classification , Cholesterol, LDL , Humans , Hypercholesterolemia/blood , Hypercholesterolemia/classification , Hyperlipoproteinemias/classification , Lipoproteins, LDL/classification , Triglycerides/blood , Triglycerides/classificationABSTRACT
There is a high morbidity from hepato-biliary disease in France. These diseases are often accompanied by a reduction in serum lecithin-cholesterol-acyl-transferase activity, which is difficult and costly to diagnose in the laboratory. Thanks to a simple and inexpensive method of selective precipitation of light lipoproteins, we have been able to establish the practical value of the determination of the LDL-phospholipids and the molar cholesterol ratio on the phospholipids in the LDL (MCPR) in familial or secondary deficiencies of Lecithin-Cholesterol-Acyl-Transferase. These conditions are characterized by a high proportion of serum phospholipids transported by the LDL (more than 60%) and by the marked reduction in the cholesterol ratio of the phospholipids in the LDL. The authors propose a classification for the commonest causes of lecithin-cholesterol-acyl-transferase deficiency.