Effects of an immunomodulatory feed additive on the development of mastitis in a mouse infection model using four bovine-origin isolates.

The goal of this study was to examine the ability of a commercially available feed additive (OmniGen-AF) to reduce mammary infections caused by a single strain of mastitic pathogens (Streptococcus uberis, Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae) and to examine the effects of the additive on markers of mammary immunity. Four experiments were completed using a murine model of bovine mastitis. Infection progression was examined using Sybr-green- and TaqMan-based quantitative PCR assays of 16S ribosomal DNA. Infection of the mammary gland with all pathogens caused rapid (24 to 48 h) appearance of pathogen DNA in mammary tissue. Provision of the feed additive for 2 weeks before infection significantly (P < 0.05) reduced the extent of pathogen DNA accumulation in models of S. uberis, E. coli and S. aureus infection. The additive was ineffective in reducing mammary infections caused by K. pneumoniae. We examined mechanisms of action of the additive through assessment of mammary concentrations of mammary myeloperoxidase (MPO), major histocompatibility complex 2 class II (MHC) and macrophage inflammatory protein-1α (MIP) messenger RNA (mRNA) concentrations and by examining serum complement C3 concentration. Infection of the mammary gland increased concentrations of MPO and MHC mRNAs (P < 0.05). Ability of the pathogen to elicit changes in mammary MPO and MHC gene expression was enhanced by the provision of the additive for 2 weeks before infection. These data imply that the additive increased the mammary inflammatory response and increased antigen presentation during a mammary infection. Value of the additive in preventing mastitis in cattle awaits additional studies using a bovine model and further evaluation of additional strains of the pathogens used in this study.

Influence of fiber form in a complete mixed ration on incidence of left displaced abomasum in postpartum dairy cows.

During the fall of 1982, increased incidence of left displaced abomasum (LDA) was seen in a group of cows fed a complete pelleted ration (3/16 inch) at the university's dairy center during the early postpartum period. Of these, 17.4% developed LDA when fed this experimental ration, whereas only 1.6% of the remaining cows in the herd developed LDA. The latter cows (as a control) were fed loose alfalfa hay, sorghum silage, and an 18% crude protein mix. Most of the fiber in the experimental diet or pelleted diet was alfalfa hay (30% as-fed basis) that was ground through a quarter-inch screen for incorporation into the pellet. Another 10% of the dietary fiber was contributed by cottonseed hulls. Thus, increased incidence of LDA may be associated with short length of the dietary fiber in the pelleted form.

Alteration of the anterior acrosome of motile bovine spermatozoa by fructose and hydrogen ion concentration.

Storing cauda epididymal spermatozoa in seminal plasma or in defined media at 1 x 10(9) spermatozoa/ml for 24 h at 4 degrees C caused swelling of the apical ridge on motile spermatozoa (SAR) provided concentrations of fructose in the range normally found in seminal plasma or comparable levels of glucose were present. Evaluation of these conditions indicated that, with glycolysable sugars in the media, pH dropped from 6.6-6.7 to 5.7-6.0. Most of the pH decrease occurred during the first 2 h of slow cooling from 37 to 4 degrees C. pH decrease was undoubtedly due to sperm organic acid production which overwhelmed the relatively weak buffering capacity of the defined media and/or seminal plasma. Inducing pH decreases with HCl in fructose-free conditions, and using NaOH to prevent a pH decrease when fructose was included in media, demonstrated that exposing spermatozoa to pH values of 5.7-6.0 and not a specific response to fructose was the major cause of SAR.

Influence of dietary protein on reproductive performance of dairy cows.

From 10 +/- 3.5 d after parturition to 149 +/- 3.5 d of lactation, 146 cows (109 Holstein and 37 Ayrshires) were fed either a 15 or 20% crude protein diet of 45% sorghum silage and 55% concentrate. Milk production was enhanced by the 20% protein diet, but percentage of milk fat and protein were unchanged. Average daily intake of DM was unaffected by diet. Actual decreases in body weight and condition were small, although cows on 15% protein lost more condition. Concentration of plasma urea nitrogen increased rapidly during the first 4 wk of the experiment with cows on the 20% protein diet maintaining a 10 mg/dl advantage after the 4th wk on experiment. Diet did not affect the postpartum interval to first normal corpus luteum and standing estrus. Cows were inseminated artificially between 55 and 145 d post-calving with one freeze batch of semen from one bull of each appropriate breed mainly by one inseminator. Overall, days open, services per conception, and percentage cows pregnant on experiment were 80, 1.40, and 85, respectively. Concentration of protein in the diet did not affect these reproductive traits. There was no conflict between the amount of dietary protein provided for lactation and reproduction in this experiment.

Morphological change of the acrosome on motile bovine spermatozoa due to storage at 4 degrees C.

Swelling of the apical ridge and anterior acrosome of motile bovine spermatozoa was observed during in-vitro storage using differential interference-contrast optics. This morphological alteration is different from that described as the false acrosome reaction on immotile spermatozoa, apparent in ageing semen samples and which has been associated with cell death. In this study, transmission electron microscopy revealed that the apical ridge acrosomal matrix was extended into complex folds and/or projections. Acrosomal and plasma membrane integrity was retained. Storing spermatozoa (1500 X 10(6)/ml) in seminal plasma at 4 degrees C for 1 day was most conducive to the swelling of the apical ridge. Replacing seminal plasma with egg yolk-citrate inhibited swelling. However, incubating semen at 37 degrees C in egg yolk-Tris-fructose extender (25 X 10(6) spermatozoa/ml) after storage in egg yolk-citrate at 4 degrees C for greater than or equal to 3 days restored the swelling characteristic.

The relationship of sperm viability and concentration to serum-induced head-to-head agglutination of bovine spermatozoa.

Individual samples of bull semen were treated by filtration to remove immotile spermatozoa and by the addition of 10% heifer serum. There was significantly (P less than 0.01) greater serum-induced agglutination in the filtered than in the unfiltered samples. Head-to-head agglutination was also greater (P less than 0.01) in dilute sperm suspensions when compared to concentrated sperm suspensions.