Lessons learned from Jordan's response to the Syria refugee crisis.

The number of refugees across the world has jumped from 15.7 million to 34.6 million in the past 10 years, and their forced displacement situation remain for an average period of 20 years. To ensure sustainable benefits, health system strengthening through development cooperation should be a priority in parallel to humanitarian aid early on in refugee crises.

Genotype and allelic frequencies of a newly identified mutation causing blindness in jordanian awassi sheep flocks.

A total of 423 blood samples were collected (during 2009 and 2010) from all the ram holdings at three major Jordanian governmental Awassi breeding stations (Al-Khanasry, Al-Mushairfa and Al-Fjaje) and two private flocks. All blood samples were screened for the presence of mutations at the CNGA3 gene (responsible for day blindness in Awassi sheep) using RFLP-PCR. The day blindness mutation was detected in all studied flocks. The overall allele and genotype frequencies of all studied flocks of the day blindness mutation were 0.088 and 17.49%, respectively. The genotype and allele frequencies were higher in station flocks than the farmer flocks (0.121, 24.15 and 0.012, 2.32, respectively). Al-Mushairfa and Al-Khanasry stations have the highest genotype and allele frequencies for the day blindness mutation that were 27.77, 30.00% and 0.14, 0.171, respectively. The investigated farmer flocks have low percentages (0.03, 5.88% at Al-Shoubak and 0.005 and 1.05%, at Al-Karak, respectively for genotype and allele frequencies) compared with the breeding stations. Ram culling strategy was applied throughout the genotyping period in order to gradually eradicate this newly identified day blindness mutation from Jordanian Breeding station, since they annually distribute a high percentage of improved rams to farmer's flocks.

Effect of sulfide concentration on the location of the metal precipitates in inversed fluidized bed reactors.

The effect of the sulfide concentration on the location of the metal precipitates within sulfate-reducing inversed fluidized bed (IFB) reactors was evaluated. Two mesophilic IFB reactors were operated for over 100 days at the same operational conditions, but with different chemical oxygen demand (COD) to SO(4)(2-) ratio (5 and 1, respectively). After a start up phase, 10mg/L of Cu, Pb, Cd and Zn each were added to the influent. The sulfide concentration in one IFB reactor reached 648 mg/L, while it reached only 59 mg/L in the other one. In the high sulfide IFB reactor, the precipitated metals were mainly located in the bulk liquid (as fines), whereas in the low sulfide IFB reactor the metal preciptiates were mainly present in the biofilm. The latter can be explained by local supersaturation due to sulfide production in the biofilm. This paper demonstrates that the sulfide concentration needs to be controlled in sulfate reducing IFB reactors to steer the location of the metal precipitates for recovery.

Expression of cytosolic phospholipase A2 in equine endometrium during the oestrous cycle and early pregnancy.

Phospholipase A2 (PLA2) is a key enzyme for biosynthesis of PGF2α. Real-time RT-PCR and immunohistochemistry were used to determine transcription and cellular distribution of cytosolic PLA2 (cPLA2) in the equine endometrium during the oestrous cycle and early pregnancy. Endometrial biopsy and blood samples were collected from cycling mares on days (d) 8, 11, 15 and 18 (oestrus) (Day 0 = Day of ovulation; n = 5 for each day) and from pregnant mares (n = 4) on d15. Except for mares on d18 and some cyclic mares (n = 2) on d15 with low progesterone (P4) concentrations (< 3.18 nm), P4 concentrations were high. Cytosolic PLA2 was mainly localized in the luminal epithelium and stroma was negative. Cytosolic PLA2 expression was negatively correlated with P4 concentration (r = -0.75, P < 0.001) and differed according to the stage of the oestrous cycle (P < 0.05). Cytosolic PLA2 expression was high during oestrus and declined to basal levels on d8 (P < 0.05). Thereafter, there was a trend for increased cPLA2 expression as luteal phase progressed. However, as P4 dropped below 3.18 nm on d15, cPLA2 expression increased (P < 0.05). In pregnant mares, cPLA2 expression was not different from cyclic mares on d15 with high P4 concentrations. However, it was lower than cyclic mares with low P4 concentrations (p < 0.05). In conclusion, cPLA2 isoform, a member of the PLA2 family that controls PGF2α secretion, is highly expressed in the endometrium at the expected time of luteolysis. Progesterone may be an inhibitor for cPLA2 expression. During pregnancy, cPLA2 expression may be sufficient to initiate the cascade for PGF2α secretion and does not play a direct role in maternal recognition of pregnancy.

The effect of the extract of the miswak (chewing sticks) used in Jordan and the Middle East on oral bacteria.

Chewing sticks are commonly used in Jordan, Saudi Arabia and the United Arab Emirates in particular, and the Middle East, Asia, and Africa in general, in addition to many other areas for oral hygiene, religious and social purposes. Recently, the World Health Organisation (WHO) has recommended and encouraged the use of these sticks as an effective tool for oral hygiene. The antibacterial activity of one of these sticks has been tested against some oral aerobic and anaerobic bacteria. Three methods of antibacterial activity were carried out: streaked plate method, ditch plate method, and tube dilution test for minimum inhibitory concentration (MIC). It was found that the extract of these sticks had a drastic effect on the growth of Staphylococcus aureus with MIC values of 69 mg/100 cc, while a variable effect on other bacterial species was noted. It is concluded that using chewing sticks twice a day on a regular basis may reduce the incidence of gingivitis and possibly dental caries. Apart from their antibacterial activity which may help control the formation and activity of dental plaque, they can be used effectively as a natural toothbrush for teeth cleaning. Such sticks are effective, inexpensive, common, available, and contain many medical properties.