ABSTRACT
AIM: Improvement of therapy of chronic ophthalmic infectious diseases during assessment of functioning of different arms of immune system. MATERIALS AND METHODS: Three hundred and fifty patients with chronic red-eye syndrome were tested by immunofluorescence assay on the presence of antigens of herpesviruses, adenoviruses and Chlamydia in samples from conjunctiva. Expression of 11 cytokines' genes was measured in peripheral blood mononuclear cells by reverse transcription polymerase chain reaction. Production of IFN-alpha and IFN-gamma, levels of serum and spontaneously produced interferon as well as level of susceptibility to the range of immunomodulating preparations were measured during study of interferon status in whole blood cells. Study of parameters of cytokine, interferon and immune statuses was performed in 70 patients. Counts of T- and B-lymphocytes, T-helpers, NK-cells as well as level of circulating immune complexes were measured during study of immune status. RESULTS: Antigens of herpes simplex virus and adenoviruses were detected in samples from conjunctiva in 27% (95 persons) and 36% (126 persons) of patients respectively. Enhanced level of expression of several cytokines (IL-2, IL-4) in studied patients compared with healthy volunteers was observed. Expression levels of IL-12 and TNF-alpha mRNAs were, in opposite, in 2 - 3 times lower. Disorder of IFN-alpha and IFN-gamma synthesis on post-transciption level was observed in 60 - 90% of patients. Decrease of absolute numbers of total T-lymphocytes and T-helpers as well as increase of absolute number of NK-cells was noted in 20%, 25%, and 27% of patients respectively. CONCLUSION: Assignment of individually oriented antiviral, antibacterial and immunomodulating therapy allowed to mitigate intensity of clinical symptoms in 30 -60% of patients with chronic persistent infections of anterior segment of eye.
Subject(s)
Adenovirus Infections, Human/drug therapy , Conjunctivitis, Bacterial/drug therapy , Conjunctivitis, Viral/drug therapy , Herpes Simplex/drug therapy , Trachoma/drug therapy , Adenovirus Infections, Human/diagnosis , Adenovirus Infections, Human/immunology , Anti-Infective Agents/therapeutic use , Antigens, Viral/analysis , Chlamydia/drug effects , Chronic Disease , Conjunctivitis, Bacterial/diagnosis , Conjunctivitis, Bacterial/immunology , Conjunctivitis, Viral/diagnosis , Conjunctivitis, Viral/immunology , Cytokines/biosynthesis , Herpes Simplex/diagnosis , Herpes Simplex/immunology , Humans , Immunologic Factors/therapeutic use , Lymphocyte Count , Trachoma/diagnosisABSTRACT
The efficiency of the combined use of amixin and anti-herpetic vaccination (AHV) to prevent the relapses of herpetic keratitis (HK) is investigated. One hundred and four patients with HK were followed up. The efficiency was found to be higher in the group of patients who received both amixin and AHV: in 29 (87.9%) patients with surface HK and in 10 (90.9%) patients with deep HK. No effect was registered in 4 (12.1%) persons with surface HK and in 1 (9.1%) person with deep HK. Amixin, when combined with AHV, cuts the number of relapses, therefore, it extends the remission period. The suggested scheme of amixin administration is as follow: 125 mg, once per week, for 10 weeks, 10 pills per one treatment course.
