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1.
Neurobiol Dis ; 14(3): 357-64, 2003 Dec.
Article in English | MEDLINE | ID: mdl-14678753

ABSTRACT

Mutations in the parkin gene are responsible for autosomal recessive parkinsonism. The disease-linked missense mutations are highly concentrated in the RING-IBR-RING domains of Parkin. In this study, we investigated the consequences of several missense parkin gene mutations in cell culture. We have demonstrated that two of these mutations (C289G and C418R), which replace consensus cysteine residues in the RING domains, significantly decrease the solubility of Parkin in cells. Upon overexpression, the presumably misfolded proteins formed cytoplasmic aggregates that concentrated into large perinuclear inclusion bodies when proteasome activity was inhibited. This process required active microtubule-dependent retrograde transport, as previously reported for aggresome formation. These results provide information on the molecular basis of the loss of function caused by mutations of critical residues in Parkin. They also contribute to our understanding of the cellular mechanism underlying the aggregation of mutant Parkin.


Subject(s)
Inclusion Bodies/genetics , Mutation, Missense/genetics , Neurons/metabolism , Parkinson Disease/genetics , Ubiquitin-Protein Ligases/metabolism , Animals , COS Cells , Cysteine Endopeptidases/metabolism , Humans , Inclusion Bodies/metabolism , Macromolecular Substances , Microtubules/metabolism , Multienzyme Complexes/metabolism , Neurons/pathology , Parkinson Disease/metabolism , Parkinson Disease/physiopathology , Proteasome Endopeptidase Complex , Protein Folding , Protein Structure, Tertiary/genetics , Protein Transport/physiology , Solubility , Ubiquitin-Protein Ligases/genetics , Ubiquitins/metabolism
2.
Hum Mol Genet ; 12(18): 2277-91, 2003 Sep 15.
Article in English | MEDLINE | ID: mdl-12915482

ABSTRACT

Mutations of the parkin gene are the most frequent cause of early onset autosomal recessive parkinsonism (EO-AR). Here we show that inactivation of the parkin gene in mice results in motor and cognitive deficits, inhibition of amphetamine-induced dopamine release and inhibition of glutamate neurotransmission. The levels of dopamine are increased in the limbic brain areas of parkin mutant mice and there is a shift towards increased metabolism of dopamine by MAO. Although there was no evidence for a reduction of nigrostriatal dopamine neurons in the parkin mutant mice, the level of dopamine transporter protein was reduced in these animals, suggesting a decreased density of dopamine terminals, or adaptative changes in the nigrostriatal dopamine system. GSH levels were increased in the striatum and fetal mesencephalic neurons from parkin mutant mice, suggesting that a compensatory mechanism may protect dopamine neurons from neuronal death. These parkin mutant mice provide a valuable tool to better understand the preclinical deficits observed in patients with PD and to characterize the mechanisms leading to the degeneration of dopamine neurons that could provide new strategies for neuroprotection.


Subject(s)
Behavior, Animal/drug effects , Dopamine/metabolism , Gene Silencing , Neurotransmitter Uptake Inhibitors/metabolism , Ubiquitin-Protein Ligases/genetics , Alleles , Animals , Base Sequence , Body Temperature/genetics , Body Weight/genetics , Catecholamines/antagonists & inhibitors , Cells, Cultured , Dopamine/pharmacokinetics , Enzyme Inhibitors/pharmacology , Exons , Female , Homozygote , Introns , Male , Mice , Mice, Transgenic , Monoamine Oxidase/metabolism , Neurons/drug effects , Neurons/metabolism , Sequence Deletion , Ubiquitin-Protein Ligases/metabolism , alpha-Methyltyrosine/pharmacology
3.
Hum Mol Genet ; 12(5): 517-26, 2003 Mar 01.
Article in English | MEDLINE | ID: mdl-12588799

ABSTRACT

Parkin gene mutations have been implicated in autosomal-recessive early-onset parkinsonism and lead to specific degeneration of dopaminergic neurons in midbrain. To investigate the role of Parkin in neuronal cell death, we overproduced this protein in PC12 cells in an inducible manner. In this cell line, neuronally differentiated by nerve growth factor, Parkin overproduction protected against cell death mediated by ceramide, but not by a variety of other cell death inducers (H(2)O(2), 4-hydroxynonenal, rotenone, 6-OHDA, tunicamycin, 2-mercaptoethanol and staurosporine). Protection was abrogated by the proteasome inhibitor epoxomicin and disease-causing variants, indicating that it was mediated by the E3 ubiquitin ligase activity of Parkin. Interestingly, Parkin acted by delaying mitochondrial swelling and subsequent cytochrome c release and caspase-3 activation observed in ceramide-mediated cell death. Subcellular fractionation demonstrated enrichment of Parkin in the mitochondrial fraction and its association with the outer mitochondrial membrane. Together, these results suggest that Parkin may promote the degradation of substrates localized in mitochondria and involved in the late mitochondrial phase of ceramide-mediated cell death. Loss of this function may underlie the degeneration of nigral dopaminergic neurons in patients with Parkin mutations.


Subject(s)
Cytochrome c Group/metabolism , Ligases/metabolism , Mitochondria/metabolism , Animals , Caspase 3 , Caspases/metabolism , Cell Death/physiology , Ceramides/metabolism , Humans , Neurons/metabolism , Rats , Ubiquitin-Protein Ligases
4.
Neurobiol Dis ; 10(1): 28-32, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12079401

ABSTRACT

In the present study, we tried to clarify the potentially protective role of Bcl-x(L), an anti-apoptotic member of the Bcl-2 family of proteins, in Parkinson's disease (PD). Using in situ hybridization on human postmortem mesencephalon sections, we show that in PD patients Bcl-x(L) mRNA expression per dopaminergic neuron was almost double that of controls. We also show that, ultrastructurally, this effect may be mediated by a redistribution of Bcl-x(L) from the cytosol to the outer mitochondrial membrane.


Subject(s)
Apoptosis/physiology , Parkinson Disease/metabolism , Proto-Oncogene Proteins c-bcl-2/biosynthesis , Aged , Aged, 80 and over , Dihydroxyphenylalanine/physiology , Humans , Melanins/metabolism , Mesencephalon/metabolism , Mesencephalon/ultrastructure , Neurons/metabolism , Neurons/physiology , Neurons/ultrastructure , Organ Specificity , Parkinson Disease/pathology , Proto-Oncogene Proteins c-bcl-2/metabolism , Proto-Oncogene Proteins c-bcl-2/ultrastructure , RNA, Messenger/biosynthesis , bcl-X Protein
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