ABSTRACT
In this study, we reported the ammonium metavanadate (NH4VO3) as an efficient, cost-effective, and mild catalyst for the synthesis of substituted pyridines via a one-pot pseudo four-component reaction. Furthermore, we investigated Hantzsch 1,4-dihydropyridines (1,4-DHPs) synthesis and oxidation of 1,4-DHPs to their corresponding pyridines. The present approach offers a rapid methodology for accessing various pyridines with broad functional group tolerance and good yields using NH4VO3 catalyst as a green catalyst.
Subject(s)
Dihydropyridines , Pyridines , Catalysis , VanadatesABSTRACT
INTRODUCTION: Escherichia coli (E.coli) as an opportunistic pathogen is a major cause of the hospital infections. The main goal of this research was to determine the frequency of quinolone resistance genes (qnr) among E.coli pathotypes isolated from patients with urinary tract infections (UTIs). MATERIAL AND METHODS: Urine samples were obtained from patients with UTIs in three major hospitals of Mofid, Bu Ali, and Vali-Asr during the year of 2015 in Tehran, Iran. The antibiogram was done for isolated bacterial isolates using nalidixic acid, norfloxacin, gentamicin, streptomycin, and chloramphenicol. Then the plasmids of the bacterial samples were extracted. PCR was used to detect qnr genes. Finally, the PCR products were run on a 1% agarose gel electrophoresis and the results were analyzed by the program SPSS version 22. RESULTS: Overall, 100 E.coli strains were isolated from patients with UTIs. The highest resistance rate was against Streptomycin. The frequency of the genes of qnrA, qnrB and qnrS were 0%, 25% and 36%, respectively. Moreover, the presence of the both genes of qnrB and qnrS was recognized in 10% of isolated bacterial strains. CONCLUSIONS: Our results indicated increasing rates of quinolone resistant E.coli strains circulating in hospitals under the study. Dissemination of these strains harboring qnr determinants is of particular concern.
