The impact of busulfan on the testicular structure in prepubertal rats: A histological, ultrastructural and immunohistochemical study.

Busulfan is a widely used cancer chemotherapeutic agent. Temporary or permanent sterility in male patients is one of the most common side effects of this drug. The present study was performed to evaluate the changes in the microscopic structure of the testes of prepubertal rats, as well as the changes in PCNA and caspase-3 immune expression, at different durations after busulfan administration. The rats were 5 weeks old and were divided into two main groups. Control group and busulfan treated group. Busulfan treated group received a single dose of busulfan (40 mg/kg), then animals were subdivided to three subgroups; IIa, IIb, IIc which were sacrificed after four, ten and twenty weeks, respectively, from the beginning of the experiment. Light and electron microscopic studies were done. Serum testosterone level and relative testes weight were assessed. Immunohistochemical staining for anti-proliferating cell nuclear antigen (PCNA) and anti-caspase-3 antigen was also done. Morphometric and statistical studies were carried out. Group II revealed histological and ultrastructural degenerative changes including congested blood vessels and degenerated spermatogenic epithelium, Sertoli cells, and Leydig cells. These changes were more evident after 10 weeks of busulfan administration and were accompanied by absence of mature sperms in the lumen of seminiferous tubules. These changes were associated with a significant reduction in relative testes weight, testosterone level, germinal epithelial height and seminiferous tubule diameter. Moreover, PCNA and caspase-3 immune expression was significantly altered in busulfan treated group. Mild improvement in testicular structure was observed 20 weeks after busulfan treatment.

The possible protective role of melatonin versus garlic on monosodium Glutamate-induced changes in rat cerebellar cortex: histological, immunohistochemical and electron microscope study.

Monosodium glutamate (MSG) is widely used as a flavor enhancer. Melatonin and garlic are well known as antioxidant. The present study was performed to evaluate the microscopic changes in the cerebellar cortex of rats after the administration of MSG and the possible protective effect of melatonin versus garlic on those changes. The rats were divided into four main groups. Group I (control group). Group II received MSG (4 mg/ g/day). Group III received MSG+ melatonin (10 mg/kg bw/day). Group IV received MSG+garlic (300 mg/kg bw/day). Immunohistochemical staining for glial fibrillary acidic protein (GFAP) was done as a marker for astrocyte demonstration. Morphometric study was done to assess the mean number and diameter of Purkinje cells, the number of astrocytes and the percentage area of positive GFAP immune stain. MSG group demonstrated congested blood vessels, vacuolations in the molecular layer, and Purkinje cells appeared irregular with nuclear degeneration. Granule cells appeared shrunken with darkly stained nuclei. The immunohistochemical stain for GFAP was less than expected in the three layers of the cerebellar cortex. Purkinje cells and granule cells appeared irregular in shape with dark small heterochromatic nuclei. The myelinated nerve fibers showed splitting and loss of the lamellar structure of their myelin sheath. Melatonin group showed that the cerebellar cortex was nearly similar to that of control group. Garlic treated group showed partial improvement. In conclusion, melatonin and garlic could partially protect against MSG induced changes and the protective effect of melatonin was better than garlic.