ABSTRACT
A cross-sectional study was conducted to evaluate the prevalence and risk factors of Toxoplasma gondii antibodies in 260 blood donors seen at blood banks, Mansoura University Hospital, Egypt. Blood donors were interviewed about sociodemographic characteristics and risk factors for T. gondii infection. A blood sample was taken to document the T. gondii antibody status by using ELISA. Overall, 155 (59.6%) of 260 blood donors were positive for anti-T. gondii IgG antibodies. Multivariate logistic regression analysis showed a significant association between T. gondii positivity and eating processed meat as luncheon and/or shawerma (adjusted odds ratio [OR] 80.82 [95% CI 18.62-350.81], P < 0.0001) or being non-educated (adjusted OR 32.25 [95% CI 7.46-139.44], P < 0.0001). These findings highlight the prevalent T. gondii among blood donors and focused on the risk factors.
Subject(s)
Antibodies, Protozoan/blood , Blood Donors , Toxoplasma/immunology , Toxoplasmosis/epidemiology , Adult , Animals , Cross-Sectional Studies , Egypt/epidemiology , Female , Humans , Male , Middle Aged , Prevalence , Risk Factors , Toxoplasmosis/etiology , Young AdultABSTRACT
The efficacy of leeches, as biological agents, in control of snail intermediate hosts of schistosomiasis (Bulinus truncatus, Biomphalaria alexandrina) and fascioliasis (Lymnaea natalensis) as well as their effect on the non-target snails Physa acuta, Melanioides tuberculata and Cleopatra bulimoides was evaluated. Two glossiphoniid snail leeches, Helobdella nilae and Alboglossiphonia conjugata were used. They destroyed egg masses and young snails more rapidly than adult ones. H. nilae showed a stronger destructive effect than A. conjugata. In a descending order, it preferred L. natalensis followed by B. truncatus, B. alexandrina, Ph. acuta, M. tuberculata and lastly C. bulimoides. But, A. conjugata preferred L. natalensis followed by B. truncatus, Ph. acuta, M. tuberculata, B. alexandrina and lastly C. bulimoides. The detailed diagnostic morphology and biology of the two leeches were given.
Subject(s)
Leeches/physiology , Pest Control, Biological/methods , Snails/physiology , Animals , Leeches/anatomy & histology , Ovum , Time FactorsABSTRACT
ELISA IgG, IgM antibodies and PCR for toxoplasmosis were performed on 55 women with complicated gestation and their babies. Besides, ELISA IgG and IgM were applied on 27 uncomplicated gestation (mothers & babies) and 152 randomly selected individuals. Seropositivity to specific IgG antibodies was 36.4%, 59.2% and 57.9% and for IgM was 27.3%, 7.4% and 10.5% in complicated gestation. uncomplicated gestation and random population respectively. PCR was positive in 20%, 50% and 60% of mothers with abortion, premature deliveries and deliveries of babies with congenital anomalies respectively. 55.5% and 40% were found seropositive for IgG from normal full term babies and abnormal babies. 13% of abnormal babies were IgM positive and 46.6% were PCR positive from the same group.
