Crystal structure and Hirshfeld surface analysis of (±)-N'-(2-hy-droxy-3-meth-oxy-benzyl-idene)-2-(4-iso-butyl-phen-yl)propionohydrazide.

The title mol-ecule, C21H26N2O3, adopts a V-shaped conformation and is chiral at the C atom with methyl group attached at the common cut of the edges of the V-conformation and crystallizes as a racemate. It also contains an intra-molecular O-H⋯N hydrogen bond. In the crystal, N-H⋯O hydrogen bonds form chains of mol-ecules extending along the c-axis direction, together with normal van der Waals contacts. The roles of the various inter-molecular inter-actions were clarified by Hirshfeld surface analysis, which reveals that the most important contributions to the crystal packing are from H⋯H (62.6%), C⋯H/H⋯C (15.8%) and O⋯H/H⋯O (15.3%) contacts.

An AI-Enabled Internet of Things Based Autism Care System for Improving Cognitive Ability of Children with Autism Spectrum Disorders.

Smart monitoring and assisted living systems for cognitive health assessment play a central role in assessment of individuals' health conditions. Autistic children suffer from some difficulties including social skills, repetitive behaviors, speech and nonverbal communication, and accommodating to the environment around them. Thus, dealing with autistic children is a serious public health problem as it is hard to determine what they feel with a lack of emotional cognitive ability. Currently, no medical treatments have been shown to cure autistic children, with most of the social assistive research to date focusing on Autism Spectrum Disorder (ASD) without suggesting a real treatment. In this paper, we focus on improving cognitive ability and daily living skills and maximizing the ability of the autistic child to function and participate positively in the community. Through utilizing intelligent systems based Artificial Intelligence (AI) and Internet of Things (IoT) technologies, we facilitate the process of adaptation to the world around the autistic children. To this end, we propose an AI-enabled IoT system embodied in a sensor for measuring the heart rate to predict the state of the child and then sending the state to the guardian with feeling and expected behavior of the child via a mobile application. Further, the system can provide a new virtual environment to help the child to be capable of improving eye contact with other people. This way is represented in pictures of these persons in 3D models that break this child's fear barrier. The system follows strategies that have focused on social communication skill development particularly at young ages to be more interactive with others.

Quantitative and qualitative evaluation of toxic metals and trace elements in the tissues of renal cell carcinoma compared with the adjacent non-cancerous and control kidney tissues.

Toxic metals and trace elements (TMTE) are linked to the development of several human cancers. Many reports have documented the association between some TMTE and renal cell carcinoma. In this work, we assessed the presence (qualitative) and evaluated the concentration (quantitative) of 22 TMTE in three groups of kidney tissue samples: renal cell carcinoma (RCC), adjacent non-cancerous, and control kidney tissues from cadavers. A total of 75 paired specimens of RCC and adjacent non-cancerous tissues were harvested immediately after radical nephrectomy and preserved in 10% diluted formalin solution. Twelve specimens, age- and sex-matched from the normal kidney tissue of the cadavers, who died from non-cancerous reasons, were collected and served as control. All tissue specimens were subjected to evaluation of TMTE concentration (22 elements in each specimen) by using the inductively coupled plasma optical emission spectrometry (ICP-OES) technique. The tumor, histopathology, stage, and grade were correlated with the concentration and types of TMTE. The results showed that the histological types of RCC were as follows: clear cell type in 35 (21.5%), chromophobe 22 (13.5%), papillary 7 (4.5%), oncocytoma 5 (3.1%), and unclassified 6 (3.7%). ICP-OES revealed that tumorous (RCC) tissues had a higher concentration of 9 TMTE (Ca, Cd, K, Mg, Mn, Na, Pb, S, and Sr) compared with both the adjacent non-cancerous and control tissue. The adjacent non-cancerous kidney tissues showed the highest concentration of Fe, K, and Na. The control of kidney tissues from cadavers had the highest level of Cu, Zn, Mo, and B compared with the cancerous and adjacent non-cancerous tissues. Female patients had higher concentrations of Zn and Cu in the non-cancerous tissues of their kidneys. Younger patients had a higher concentration of B in the adjacent non-cancerous, and higher Cu in the cancerous tissues. Cadmium concentration was highest in the chromophobe cell type of RCC compared with other subtypes. There was no correlation between the TMTE concentration and the pathological stage of RCC.

Therapeutic Role of Transcranial Direct Current Stimulation in Alzheimer Disease Patients: Double-Blind, Placebo-Controlled Clinical Trial.

OBJECTIVE: To explore the neuropsychological effects and levels of tau protein (TAU), amyloid ß 1-42 (Aß 1-42), and lipid peroxidase after 10 sessions of anodal transcranial direct current stimulation (tDCS) in patients with mild to moderate Alzheimer disease (AD). PATIENTS AND METHODS: A total of 46 consecutive patients with probable AD participated in this study. They were classified randomly into 2 equal groups: active versus sham. Each patient received 10 sessions of anodal tDCS over the left and right temporoparietal region for 20 minutes for each side with the cathode on the left arm. Patients were assessed using the Modified Mini Mental State Examination (MMMSE), clock drawing test, Montreal Cognitive Scale (MoCA), and the Cornell Scale for depression. Serum TAU, Aß 1-42, and lipid peroxidase were measured before and after the 10th session. RESULTS: There was a significant improvement in the total score of each cognitive rating scale (MMMSE, clock drawing test, and MoCA) in the real group, whereas no such change was observed in the sham group. The Cornell depression score improved significantly in both groups. There was a significant increase in serum Aß 1-42 ( P = .02) in the real but not in the sham group, with a significant Treatment condition × Time interaction ( P = .009). There was no significant effect on tau or lipid peroxidase in either group but a significant positive correlation between changes of Aß1-42 and MMMSE ( P = .005) and MoCA ( P = .02). CONCLUSION: The observed cognitive improvements were complemented by parallel changes in serum levels of Aß 1-42.

Value of serum anti-p53 antibodies as a prognostic factor in Egyptian patients with hepatocellular carcinoma.

OBJECTIVE: p53 antigen is an oncoprotective antigen and when damaged, leads to production of anti-p53 and also predisposes to various cancers, including hepatocellular carcinoma (HCC). Serum anti-p53 has been proven to have a prognostic value in patients with HCC. The objective of this study was to determine the prevalence and prognostic utility of serum anti-p53 in Egyptian patients with HCC. METHODS: Forty one patients with HCC, 26 patients with liver cirrhosis and 29 healthy controls were included in this study. For all the studied groups, we studied the clinical data, abdominal ultrasound (US) findings, biochemical liver function tests, serum alpha-fetoprotein (AFP) levels detected by enzyme immunoassay (EIA) kit and anti-p53 antibody levels by a modified enzyme-linked immunosorbent assay (ELISA). The severity of liver disease was assessed by Child-Pugh and MELD scores. Tumor characteristics were detected by (US) with or without computed tomography (CT) scan. These characteristics included tumor size, number and site. Tumor staging was done using Okuda, Cancer Liver Italian Program (CLIP) and Tokyo staging systems. Also, the overall survival of patients with HCC with reference to p53 antibody level was studied. RESULTS: The mean age of HCC patients was 57.95+/-8.41. There was a male predominance among HCC patients with male-to-female ratio of 3.6:1. Anti-p53 antibodies were detected in the sera of 68.3% of HCC patients, 50% of liver cirrhosis patients and 17.2% of healthy control subjects. The data showed that HCC patients had a significantly higher mean anti-p53 antibody values (p=0.0001), than both liver cirrhosis patients and healthy control groups. Our results revealed that anti-p53 has a positive significant correlation with AFP (p=0.002), severity of liver disease [Child Pugh score (p=0.02) and MELD score (p=0.0003)], tumor size (p<0.0001), tumor number (p=0.003) and tumor staging systems [Okuda (p=0.04), CLIP (p=0.006) and Tokyo (p<0.0001)]. Also, our results revealed that serum anti-p53 antibodies had a significant association with overall survival of patients with HCC (p=0.019) with a shorter survival time in anti-p53 positive status patients and with higher anti-p53 antibody levels within 19 months follow up. CONCLUSION: The detection of anti-p53 antibodies may be suitable for assessing the prognosis of HCC patients. The higher percentage of positivity of anti-p53 antibodies in Egyptian control subjects than reported elsewhere needs further thorough investigation.

Chromosomal aberrations in benign and malignant bilharzia-associated bladder lesions analyzed by comparative genomic hybridization.

BACKGROUND: Bilharzia-associated bladder cancer (BAC) is a major health problem in countries where urinary schistosomiasis is endemic. Characterization of the genetic alterations in this cancer might enhance our understanding of the pathogenic mechanisms of the disease but, in contrast to nonbilharzia bladder cancer, BAC has rarely been the object of such scrutiny. In the present study, we aimed to characterize chromosomal imbalances in benign and malignant post-bilharzial lesions, and to determine whether their unique etiology yields a distinct cytogenetic profile as compared to chemically induced bladder tumors. METHODS: DNAs from 20 archival paraffin-embedded post-bilharzial bladder lesions (6 benign and 14 malignant) obtained from Sudanese patients (12 males and 8 females) with a history of urinary bilharziasis were investigated for chromosomal imbalances using comparative genomic hybridization (CGH). Subsequent FISH analysis with pericentromeric probes was performed on paraffin sections of the same cases to confirm the CGH results. RESULTS: Seven of the 20 lesions (6 carcinomas and one granuloma) showed chromosomal imbalances varying from 1 to 6 changes. The most common chromosomal imbalances detected were losses of 1p21-31, 8p21-pter, and 9p and gain of 19p material, seen in three cases each, including the benign lesion. CONCLUSION: Most of the detected imbalances have been repeatedly reported in non-bilharzial bladder carcinomas, suggesting that the cytogenetic profiles of chemical- and bilharzia-induced carcinomas are largely similar. However, loss of 9p seems to be more ubiquitous in BAC than in bladder cancer in industrialized countries.