ABSTRACT
Wet mount is the basic primary technique for stool examination in laboratories, allowing only the use of X10 and X40 objectives which do not sometimes reveal relevant details to make an exact identification of certain protozoa. In a modification of the Glycerol Jelly (GJ) reagent, which is used in permanent preparation of helminths, five concentrations were prepared and tested for fixing the cover glass instantly while maintaining the high translucency of the fecal films. GJ reagent (7 gm gelatine dissolved in 50 ml boiling water added to 10 ml glycerol) gave satisfactory results especially with iodine and alkaline Methylene blue mounts which stained the cytological structures of protozoa while the GJ reagent enabled the examiner to use X100 oil immersion objective immediately and consequently identify protozoa with certainty and make an accurate identification. Identification of Cryptosporidium parvum oocysts by GJ wet mount, inspite of its small size, was the most impressive. GJ fecal films were examined up to 8 weeks of preparation and they were valid and reliable. GJ mount is an easy, fast and cheap technique for examining the fecal direct smear with the oil-immersion lense.
Subject(s)
Eukaryota/isolation & purification , Feces/parasitology , Fixatives , Glycerol , Parasitology/methods , Animals , Humans , Indicators and ReagentsABSTRACT
Serodiagnosis of giardiasis by counterimmunoelectrophoresis assay (CIEP) and indirect immunofluorescence test (IFAT) were evaluated versus stool examination. Giardia lamblia antibodies were detected in sera of 46.9% and 53.1% using CIEP and IFAT respectively out of 49 giardiasis-infected children (5-10 years) diagnosed microscopically. Among the control group (22 children) 13.6% and 18.2% were positive by CIEP and IFAT respectively. The findings of the study showed that we can not rely on serology to diagnose giardiasis specially in cases with low excretion rates.
Subject(s)
Antigens, Protozoan/blood , Counterimmunoelectrophoresis , Fluorescent Antibody Technique , Giardia lamblia/immunology , Giardiasis/diagnosis , Animals , Child , Child, Preschool , Evaluation Studies as Topic , Feces/parasitology , Humans , Sensitivity and SpecificityABSTRACT
An enzyme linked immunosorbent assay (ELISA) was evaluated in relation to an indirect haemagglutination (IHA) test in schistosomiasis patients who were classified by clinical, sonographic and direct methods of diagnosis. Sensitivities of ELISA and IHA respectively proved to be 100% and 69.23% in acute simple intestinal schistosomiasis; 95.5% and 90.4% in chronic active schistosomiasis patients; 86.06% and 67.41% in patients with past history of exposure; 80% and 64.28% in patients with hepatosplenomegaly with past history of schistosomiasis, and 96% and 80% in patients with hepatic fibrosis as shown by sonar. It was apparent that ELISA is more sensitive than IHA in acute simple schistosomiasis in patients with past history of exposure, those with bilharzial hepatosplenomegaly and those with hepatic fibrosis. Both tests were nearly equally sensitive in chronic active schistosomiasis.
