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1.
Nat Prod Res ; 28(17): 1335-42, 2014.
Article in English | MEDLINE | ID: mdl-24697339

ABSTRACT

Bioassay-guided isolation of methanol extract of Ruta graveolens L. leaves yielded a new quinoline alkaloid, (4S) 1,4-dihydro-4-methoxy-1,4-dimethyl-3-(3-methylbut-2-enyl)quinoline 2,7-diol, and nine phenolic compounds including rutin as a major compound. Structures of the isolated compounds were determined by using chromatography, UV, HR-ESI-MS and 1D/2D (1)H/(13)C NMR spectroscopy. The uterotonic activity of methanol extract fractions (ethyl acetate, n-butanol and aqueous fraction) as well as the isolated major compounds was tested in the isolated mouse uterus in vitro. The n-butanol-soluble fraction was found to demonstrate the most potent uterotonic activity in a dose-dependent manner, also the major isolated compound rutin revealed the occurrence of an uterotonic response, which was maximum at a concentration level of 0.25 mg/mL, accounting for 68.7% of that exhibited by the chosen concentration of oxytocin.


Subject(s)
Alkaloids/isolation & purification , Alkaloids/pharmacology , Quinolines/isolation & purification , Quinolines/pharmacology , Reproduction/drug effects , Ruta/chemistry , 1-Butanol/chemistry , Alkaloids/chemistry , Animals , Dose-Response Relationship, Drug , Egypt , Female , Mice , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Oxytocin/analysis , Plant Leaves/chemistry , Quinolines/chemistry , Rats, Sprague-Dawley , Rutin/analysis
2.
Z Naturforsch C J Biosci ; 66(9-10): 453-9, 2011.
Article in English | MEDLINE | ID: mdl-22191209

ABSTRACT

Graded concentrations (0.1-100 mg/mL reaction mixture) of the methanolic extract of the flowers of Hibiscus rosa-sinensis Linn., its water-soluble fraction as well as compounds isolated from this fraction were tested for their inhibitory effect on alkaline phosphatase enzyme activity in vitro. Both the methanolic extract and its water-soluble fraction showed significant inhibitory effects on the enzyme activity in vitro. On screening the activity of the compounds isolated from the water-soluble fraction, its high inhibitory activity was attributed to the presence of quercetin-7-O-galactoside which showed a high potent inhibition of the enzyme activity reaching 100% at 100 mg/mL reaction mixture. Phytochemical investigations of the water-soluble fraction were also carried out and afforded ten polyphenolic compounds including two new natural compounds, namely kaempferol-7-O-[6'''-O-p-hydroxybenzoyl-beta-D-glucosyl-(1-->6)-beta-D-glucopyranoside] and scutellarein-6-O-alpha-L-rhamnopyranoside-8-C-beta-D-glucopyranoside). The chemical structure of the isolated compounds was elucidated on the basis of chemical and spectral data.


Subject(s)
Alkaline Phosphatase/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Flowers/chemistry , Hibiscus/chemistry , Plant Extracts/pharmacology , Polyphenols/pharmacology , In Vitro Techniques , Magnetic Resonance Spectroscopy , Polyphenols/isolation & purification , Ultraviolet Rays
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