ABSTRACT
Bacterial cellulose (BC) and heparin-modified bacterial cellulose (HBC) were utilized to enhance the biocompatibility of highly thrombogenic PVC-based potassium and calcium membrane electrodes. Three types of membrane electrodes were prepared: (1) conventional PVC electrode (control), (2) PVC-based electrode sandwiched with bacterial cellulose membrane (BC-PVC), and (3) PVC-based electrode sandwiched with heparin-modified bacterial cellulose membrane (HBC-PVC). The potentiometric response characteristics of the modified potassium and calcium membrane electrodes (BC-PVC and HBC-PVC) were compared with those of the control PVC-based potassium and calcium selective electrode, respectively. Response characteristics of the modified membrane electrodes were comparable to the control PVC membrane electrode. The platelet adhesion investigations indicated that (BC) and (HBC) layers are less thrombogenic compared to PVC. Therefore, use of BC or HBC would enable the enhancement of the biocompatibility of PVC-based membrane electrodes for potassium and calcium while practically maintaining the overall electrochemical performance of the PVC sensing film.
Subject(s)
Biocompatible Materials/chemistry , Cellulose/chemistry , Gluconacetobacter xylinus/chemistry , Heparin/chemistry , Animals , Biocompatible Materials/pharmacology , Electrodes , Membranes, Artificial , Platelet Aggregation/drug effects , Potassium/chemistry , SheepABSTRACT
Heparin-modified chitosan (H-chitosan) membrane was utilized to enhance biocompatibility of sodium selective membrane electrode based on the highly thrombogenic polyvinyl chloride (PVC). Sodium ion sensing film was prepared using PVC, sodium ionophore-X, potassium tetrakis(chlorophenyl)-borate, and o-nitrophenyloctylether. The PVC-based sensing film was sandwiched to chitosan or H-chitosan to prevent platelet adhesion on the surface of PVC. Potentiometric response characteristics of PVC-chitosan and PVC-H-chitosan membrane electrodes were found to be comparable to that of a control PVC based sodium-selective electrode. This indicates that chitosan and H-chitosan layers do not alter the response behaviour of the PVC-based sensing film. Biocompatibility of H-chitosan was confirmed by in vitro platelet adhesion study. The platelet adhesion investigations indicated that H-chitosan film is less thrombogenic compared to PVC, which could result in enhancement of biocompatibility of sodium selective membrane electrodes based on PVC, while maintaining the overall electrochemical performance of the PVC-based sensing film.
Subject(s)
Chitosan/chemistry , Hemostatics/chemistry , Heparin/chemistry , Polyvinyl Chloride/chemistry , Potentiometry/instrumentation , Sodium/analysis , Animals , Blood Platelets/cytology , Blood Platelets/drug effects , Cations, Monovalent , Chitosan/analogs & derivatives , Chitosan/pharmacology , Electrodes , Ethers/chemistry , Membranes, Artificial , Platelet Adhesiveness/drug effects , Potentiometry/methods , Sheep, Domestic , Sodium Ionophores/chemistry , Tetraphenylborate/analogs & derivatives , Tetraphenylborate/chemistryABSTRACT
Occult hepatitis B virus (HBV) in blood donors is considered as a potential risk for transmission of HBV infection. The aim of this study was to determine the prevalence of anti-hepatitis B core antibody (anti-HBC) positivity in Egyptian blood donations as well as to estimate the frequency of HBV-DNA in anti-HBc-positive donations. The study included 760 Egyptian healthy blood donors, representing 26 different Egyptian governorates screened according to routine practice for the presence of hepatitis B surface antigen (HBsAg), hepatitis C virus (HCV) antibodies (Abs), HIV-1/2 Abs and Treponema Abs. The accepted blood units for donation were tested for the presence of total anti-HBc Abs by two tests. Positive units for anti-HBc were further tested for HBV-DNA by polymerase chain reaction. According to routine screening, a total of 48/760 units (6.3%) were rejected [38 (5%) HCV-Ab-positive units, 9 (1.18%) HbsAg-positive units and 1 (0.13%) Treponema-Ab-positive unit]. Among the accepted blood units for donation, prevalence of anti-HBc was 78/712 units (10.96%). HBV-DNA was detected in 9/78 (11.54%) of the anti-HBc-positive units, and thus, occult HBV infection was detected in 9/712 (1.26%) of the accepted blood donations. Implementing anti-HBc test to the routine assay for the forthcoming two decades would certainly eliminate possible HBV-infected units. Rejection of these units will be beneficial to decrease the risk of HBV transmission with its potential consequences particularly in immunocompromised recipients.
