Low mercury risks in paddy soils across the Pakistan.

Mercury (Hg) is a globally distributed heavy metal. Here, we study Hg concentration and isotopic composition to understand the status of Hg pollution and its sources in Pakistan's paddy soil. The collected paddy soils (n = 500) across the country have an average THg concentration of 22.30 ± 21.74 ng/g. This low mean concentration suggests Hg pollution in Pakistan was not as severe as previously thought. Meanwhile, samples collected near brick kilns and industrial areas were significantly higher in THg than others, suggesting the influence of Hg emitted from point sources in certain areas. Soil physicochemical properties showed typical characteristic of mineral soils due to the study area's arid to semi-arid climate. Hg stable isotopes analysis, depicted mean Δ199Hg of -0.05 ± 0.12‰ and mean δ202Hg -0.45 ± 0.35‰, respectively, for contaminated sites, depicting Hg was primarily sourced from coal combustion by local anthropogenic sources. While uncontaminated sites show mean Δ199Hg of 0.15 ± 0.08‰, mean Δ200Hg of 0.06 ± 0.07‰ and mean δ202Hg of -0.32 ± 0.28‰, implying long-range transboundry Hg transport through wet Hg(II) deposition as a dominant Hg source. This study fills a significant knowledge gap regarding the Hg pollution status in Pakistan and suggests that the Hg risk in Pakistan paddies is generally low.

Mining-impacted rice paddies select for Archaeal methylators and reveal a putative (Archaeal) regulator of mercury methylation.

Methylmercury (MeHg) is a microbially produced neurotoxin derived from inorganic mercury (Hg), which accumulation in rice represents a major health concern to humans. However, the microbial control of MeHg dynamics in the environment remains elusive. Here, leveraging three rice paddy fields with distinct concentrations of Hg (Total Hg (THg): 0.21-513 mg kg-1 dry wt. soil; MeHg: 1.21-6.82 ng g-1 dry wt. soil), we resorted to metagenomics to determine the microbial determinants involved in MeHg production under contrasted contamination settings. We show that Hg methylating Archaea, along with methane-cycling genes, were enriched in severely contaminated paddy soils. Metagenome-resolved Genomes of novel putative Hg methylators belonging to Nitrospinota (UBA7883), with poorly resolved taxonomy despite high completeness, showed evidence of facultative anaerobic metabolism and adaptations to fluctuating redox potential. Furthermore, we found evidence of environmental filtering effects that influenced the phylogenies of not only hgcA genes under different THg concentrations, but also of two housekeeping genes, rpoB and glnA, highlighting the need for further experimental validation of whether THg drives the evolution of hgcAB. Finally, assessment of the genomic environment surrounding hgcAB suggests that this gene pair may be regulated by an archaeal toxin-antitoxin (TA) system, instead of the more frequently found arsR-like genes in bacterial methylators. This suggests the presence of distinct hgcAB regulation systems in bacteria and archaea. Our results support the emerging role of Archaea in MeHg cycling under mining-impacted environments and shed light on the differential control of the expression of genes involved in MeHg formation between Archaea and Bacteria.

DOM influences Hg methylation in paddy soils across a Hg contamination gradient.

Rice paddies provide optimum conditions for Hg methylation, and paddy soil is a hot spot for Hg methylation and the predominant source of methylmercury (MeHg) accumulated in rice grains. The role of dissolved organic matter (DOM) in controlling Hg bioavailability and methylation in rice paddy systems remains unclear. Paddy soils from eight various cultivation sites in China were chosen to investigate the variations in soil DOM and the influence of DOM concentration and optical characteristics on Hg methylation in rice paddy systems. In the present study, 151 rhizosphere soil samples were collected, and UV-Vis absorption and fluorescent spectroscopy were used to identify the optical properties of DOM. The relationship between MeHg and DOM's optical property indices revealed the production of MeHg consumes lower molecular weight DOM. Moreover, the correlation between DOM concentration and its optical characteristics highlighted the significant role of humic components on MeHg variability in paddy soil. Variation and correlation results demonstrated the allochthonous origin of DOM in the Hg-contaminated soil, with a higher molecular weight and humic character of DOM, as well as the dominant role of autochthonous DOM in promoting Hg methylation in uncontaminated soil. The current study indicated that soil organic matter and its dissolved fractions tend to limit Hg bioavailability and subsequently diminish MeHg production in contaminated paddy soils. Furthermore, the leading roles of allochthonous DOM in protecting MeHg from degradation and autochthonous DOM signatures in enhancing MeHg production in paddy soils. Overall, these findings provide insight into the correlative distributions of DOM and Hg along a Hg concentration gradient in paddy soil, thereby highlighting their potential role in controlling Hg bioavailability and regulating Hg methylation in the soil ecosystems.

Mercury drives microbial community assembly and ecosystem multifunctionality across a Hg contamination gradient in rice paddies.

Soil microbial communities are critical for maintaining terrestrial ecosystems and fundamental ecological processes. Mercury (Hg) is a heavy metal that is toxic to microorganisms, but its effects on microbial community assembly and ecosystem multifunctionality in rice paddy ecosystems remain largely unknown. In the current study, we analyzed the microbial community structure and ecosystem multifunctionality of paddy soils across a Hg contamination gradient. The results demonstrated that Hg contamination significantly altered the microbial community structure. The microbial communities were predominantly driven by deterministic selection rather than stochastic processes. The random forest model and variation partition analysis demonstrated that the Hg level was the most important predictor of microbial profiles. Ecosystem multifunctionality decreased across the Hg concentration gradient, and multifunctionality was significantly correlated with soil biodiversity, suggesting that Hg-induced reductions in soil biodiversity led to reduced ecosystem services. A structural equation model showed that Hg contamination directly and indirectly affected ecosystem multifunctionality. The present work broadens our knowledge of the assembly of the microbiome in rice paddies across a Hg contamination gradient and highlights the significance of soil biodiversity in regulating ecosystem functions, especially in Hg-polluted rice paddies.

Uncovering geochemical fractionation of the newly deposited Hg in paddy soil using a stable isotope tracer.

The newly deposited mercury (Hg) is more readily methylated to methylmercury (MeHg) than native Hg in paddy soil. However, the biogeochemical processes of the newly deposited Hg in soil are still unknown. Here, a field experimental plot together with a stable Hg isotope tracing technique was used to demonstrate the geochemical fractionation (partitioning and redistribution) of the newly deposited Hg in paddy soils during the rice-growing period. We showed that the majority of Hg tracer (200Hg, 115.09 ± 0.36 µg kg-1) was partitioned as organic matter bound 200Hg (84.6-89.4%), followed by residual 200Hg (7.6-8.1%), Fe/Mn oxides bound 200Hg (2.8-7.2%), soluble and exchangeable 200Hg (0.05-0.2%), and carbonates bound 200Hg (0.04-0.07%) in paddy soils. Correlation analysis and partial least squares path modeling revealed that the coupling of autochthonous dissolved organic matter and poorly crystalline Fe (oxyhydr)oxides played a predominant role in controlling the redistribution of the newly deposited Hg among geochemical fractions (i.e., fraction changes). The expected aging processes of the newly deposited Hg were absent, potentially explaining the high bioavailability of these Hg in paddy soil. This study implies that other Hg pools (e.g., organic matter bound Hg) should be considered instead of merely soluble Hg pools when evaluating the environmental risks of Hg from atmospheric depositions.

Unravelling the interactive effect of soil and atmospheric mercury influencing mercury distribution and accumulation in the soil-rice system.

Mercury (Hg) accumulation in rice is an emerging health concern worldwide. However, sources and interactions responsible for Hg species accumulation in different rice tissues are still uncertain. Four experimental plots were carefully designed at an artisanal Hg mining site and a control site to evaluate the effect of atmospheric and soil Hg contents on Hg accumulation in rice. We showed that inorganic Hg (IHg) contents in rice tissues grown either in contaminated or control site soil (non-contaminated soil) were higher at Hg artisanal mining site than those at the control site. Elevated total gaseous mercury (TGM) levels in ambient air were the predominant source of IHg to rice at the Hg mining area. Methylmercury (MeHg) concentrations in rice plant tissues increased in proportionality with MeHg contents in paddy soil. Our results suggest that both atmosphere and soil Hg sources have been impacted the IHg accumulation in rice. Above ground rice tissues, grains, leaves, and stalk accumulated IHg from both atmosphere and soil to varying degrees. Nonetheless, the study also provides the first direct evidence that atmospheric Hg accumulated by above-ground rice tissues could be translocated to below-ground tissues (roots). However, the extent to which atmosphere or soil Hg contributes to IHg in rice tissues may vary with each source's concentration gradient at the given site. No evidence of in planta Hg methylation was found during the current study. Hence, paddy fields are potential MeHg production sites, whereas paddy soil is a unique MeHg accumulation source in rice plants. This study expands and clarifies the contribution of various sources involved in Hg accumulation in the soil rice system. The findings here provide the basis for future research strategies to deal with the global issue of Hg contaminated rice.

Selenium-amended biochar mitigates inorganic mercury and methylmercury accumulation in rice (Oryza sativa L.).

Rice, as a dominant crop in China and Asia, can be a major route of methylmercury (MeHg) exposure for humans in inland China, especially in those living in mercury (Hg) polluted areas. Soil is the most prominent MeHg accumulation source for rice grains. The development of management practices to reduce MeHg in rice grains is crucial. This study explored the mitigation effect of biochar (BC) and sodium selenite-amended biochar (BC + Se) on MeHg production in paddy soil and accumulation in rice. Mercury-contaminated soil was treated with 1% and 5% of both BC and BC + Se. Soil MeHg concentration slightly increased under 1% BC/BC + Se compared to control soil but decreased at the rate of 5%. Moreover, soil phytoavailable MeHg (P-MeHg) diminished as the amount of Se-amended BC increased. BC + Se effectively mitigated MeHg accumulation in rice grains. The highest average contents of MeHg and inorganic Hg (IHg) in rice seeds were found in the control samples, followed by the 1%-BC, 5%-BC, 1%-BC + Se, and 5%-BC + Se samples. Under the 5%-BC + Se treatment, rice MeHg levels were reduced significantly (94%) compared to the control, and P-MeHg concentrations in soil were lower than all the other experimental groups throughout the rice-growing season. These results demonstrate the effectiveness of BC + Se in reducing MeHg and IHg accumulation in rice and could be employed for remediation of Hg polluted paddies.

Shallow groundwater environmental investigation at northeastern Cairo, Egypt: quality and photo-treatment evaluation.

Groundwater represents the primary source of freshwater for more than 35% of world people, and its contamination became a worldwide challenge. Egypt is suffering from water quantity and quality, especially in desert areas. El Obour city and environs Northeast Cairo face waterlogging owing to the elevated-shallow groundwater table. In the present research work, the water quality of the shallow groundwater aquifer was studied. The remediation efficiency of polluted water using photocatalytic treatment technique in the presence of modified nano-titania and solar radiation has also been investigated. Twenty-eight representative samples have been collected from different locations, and their microbial, physical, and chemical characteristics were determined. The average contents of Pb (214.96 µg/L), As (1517 µg/L), Cd (8.79 µg/L), total bacterial count (2.22 × 105 CFU/ml), and bacterial indicators (MPN-index/100 ml): total coliform (497.4), fecal coliform (358.3), and fecal streptococci (115.9) were higher than WHO permissible limits for drinking water, possibly due to higher industrialization, agricultural, and urbanization rates. The organic pollutants reached critical concentrations (chemical oxygen demand up to 960.8 mg O2/L). Most of the studied samples contained acceptable concentrations of the major ions, (e.g., K+, Mg2+, HCO3-), for drinking and irrigation purposes. The statistical analyses (e.g., principal component analysis and cluster analysis) pointed out the control of water-rock interaction and anthropogenic activities in water composition. The hydrochemical data show that most of the water samples (96.4%) are Na2SO4 and NaHCO3 type, indicating its meteoric origin. The contamination with human and animal fecal substances, NO3¯, and NH4+ was identified in all samples, which pointed out the control of anthropogenic activities in water pollution. The photocatalytic technique efficiently eliminated more than 82-95% of organic contents and microbial pollutants, respectively, but it was inefficient in reducing heavy metal levels. According to the current results, shallow groundwater injection into the deep aquifer must be constrained and reusable after treatment. Finally, more studies are imperative to disseminate the applied treatment techniques to elude bacteria and organic pollutants from water at a pilot scale.

Chemical and bacterial quality monitoring of the Nile River water and associated health risks in Qena-Sohag sector, Egypt.

The River Nile is the primary source of freshwater for drinking, irrigation, and industrial purposes in Egypt. Thus, the water quality in this river concerns the health of local inhabitants. The present study reveals seasonal variations of various physicochemical and heavy metals parameters and microbial load of water at 15 sites from Qena to Sohag cities, Egypt. The water is fresh with TDS ≤ 270 and 410 mg L-1 in summer and winter, respectively. Fe, Mn, Cd, Cr, Cu, Ni, and Zn concentrations were within drinking water specification in both seasons except Cr and Cd in summer. Viable numbers of total coliform, fecal coliform, and fecal streptococci were recorded in both seasons with fecal streptococci's disappearing in winter. The concentrations of salts and ions in winter were higher than summer due to decreased water quantity and flow rate in this season. On the other hand, heavy metals and bacteria were higher in summer owing to the rain and weathering of upstream rocks and increasing of human activities during the summer. The calculated water quality index (WQI) depicted that the chemical quality of water was poor for drinking and treatment, especially biological treatment, which is required before the water is supplied for drinking. Human health risk assessment factors such as probable daily intake, hazard quotient, and carcinogenic risk indicated high risks of Cr, Cd, and Ni for adults and children in both seasons. The non-carcinogenic and carcinogenic risks are mainly posed by Cr. The WQI values for the other water uses indicated the marginal quality for aquatic life, fair for irrigation, and fair in summer to good in winter for livestock consumption. The irrigation water quality parameters indicated that the water could be used to irrigate all soils and crops except the hazard of biological contamination. The water-rock interaction controls water chemistry besides the contribution of human activities. The agricultural, industrial, and municipal wastewaters were the main contributors to water pollution and should be treated before discharge into the Nile River. Source and drinking water should be monitored continuously to prevent related human waterborne diseases.