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1.
Pain ; 157(11): 2561-2570, 2016 11.
Article in English | MEDLINE | ID: mdl-27437788

ABSTRACT

Cold exposure and a variety of types of mild stress increase pain in patients with painful disorders such as fibromyalgia syndrome. Acutely, stress induces thermogenesis by increasing sympathetic activation of beta-3 (ß3) adrenergic receptors in brown adipose tissue. Chronic stress leads to the hypertrophy of brown adipose, a phenomenon termed adaptive thermogenesis. Based on the innervation of skeletal muscle by collaterals of nerves projecting to brown adipose, we theorized an association between brown adipose tissue activity and musculoskeletal hyperalgesia and tested this hypothesis in mice. Exposure to a cold swim or injection of BRL37344 (ß3 adrenergic agonist) each enhanced musculoskeletal hyperalgesia, as indicated by morphine-sensitive decreases in grip force responses, whereas SR59230A (ß3 adrenergic antagonist) attenuated swim-induced hyperalgesia. Chemical ablation of interscapular brown adipose, using Rose Bengal, attenuated the development of hyperalgesia in response to either swim stress or BRL37344. In addition, elimination of the gene expressing uncoupling protein-1 (UCP1), the enzyme responsible for thermogenesis, prevented musculoskeletal hyperalgesia in response to either a swim or BRL37344, as documented in UCP1-knockout (UCP1-KO) mice compared with wild-type controls. Together, these data provide a convergence of evidence suggesting that activation of brown adipose contributes to stress-induced musculoskeletal hyperalgesia.


Subject(s)
Adipose Tissue, Brown/pathology , Hyperalgesia/etiology , Hyperalgesia/pathology , Musculoskeletal Pain/complications , Adipose Tissue, Brown/drug effects , Adrenergic beta-Agonists/toxicity , Animals , Body Temperature/drug effects , Body Temperature/genetics , Body Weight/drug effects , Body Weight/genetics , Cold Temperature/adverse effects , Disease Models, Animal , Ethanolamines/toxicity , Female , Hyperalgesia/genetics , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Muscle Strength/drug effects , Musculoskeletal Pain/pathology , Musculoskeletal Pain/surgery , Pain Threshold/drug effects , Pain Threshold/physiology , Reaction Time/drug effects , Reaction Time/physiology , Swimming/psychology , Tail/innervation , Uncoupling Protein 1/deficiency , Uncoupling Protein 1/genetics
2.
J Pain ; 17(10): 1081-1094, 2016 10.
Article in English | MEDLINE | ID: mdl-27377621

ABSTRACT

UNLABELLED: Macrophages play a role in innate immunity within the body, are located in muscle tissue, and can release inflammatory cytokines that sensitize local nociceptors. In this study we investigate the role of resident macrophages in the noninflammatory muscle pain model induced by 2 pH 4.0 preservative-free sterile saline (pH 4.0) injections 5 days apart in the gastrocnemius muscle. We showed that injecting 2 pH 4.0 injections into the gastrocnemius muscle increased the number of local muscle macrophages, and depleting muscle macrophages with clodronate liposomes before acid injections attenuated the hyperalgesia produced by this model. To further examine the contribution of local macrophages to this hyperalgesia, we injected mice intramuscularly with C34, a toll-like receptor 4 (TLR4) antagonist. When given before the first pH 4.0 injection, C34 attenuated the muscle and tactile hyperalgesia produced by the model. However, when given before the second injection C34 had no effect on the development of hyperalgesia. Then to test whether activation of local macrophages sensitizes nociceptors to normally non-nociceptive stimuli we replaced either the first or second acid injection with the immune cell activator lipopolysaccharide, or the inflammatory cytokine interleukin (IL)-6. Injecting LPS or IL-6 instead of the either the first or second pH 4.0 injection resulted in a dose-dependent increase in paw withdrawal responses and decrease in muscle withdrawal thresholds. The highest doses of LPS and IL-6 resulted in development of hyperalgesia bilaterally. The present study showed that resident macrophages in muscle are key to development of chronic muscle pain. PERSPECTIVE: This article presents evidence for the role of macrophages in the development of chronic muscle pain using a mouse model. These data suggest that macrophages could be a potential therapeutic target to prevent transition of acute to chronic muscle pain particularly in tissue acidosis conditions.


Subject(s)
Chronic Pain/physiopathology , Hyperalgesia/physiopathology , Macrophages/physiology , Myalgia/physiopathology , Animals , Chronic Pain/pathology , Disease Models, Animal , Female , Hydrogen-Ion Concentration , Hyperalgesia/pathology , Immunohistochemistry , Interleukin-6 , Lipopolysaccharides , Macrophages/pathology , Male , Mice, Inbred C57BL , Muscle, Skeletal/pathology , Muscle, Skeletal/physiopathology , Myalgia/pathology , Nociceptors/physiology , Toll-Like Receptor 4/antagonists & inhibitors , Toll-Like Receptor 4/metabolism
3.
Physiology (Bethesda) ; 30(6): 449-59, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26525344

ABSTRACT

Chronic musculoskeletal pain is debilitating and affects ∼ 20% of adults. Tissue acidosis is present in painful musculoskeletal diseases like rheumatoid arthritis. ASICs are located on skeletal muscle and joint nociceptors as well as on nonneuronal cells in the muscles and joints, where they mediate nociception. This review discusses the properties of different types of ASICs, factors affecting their pH sensitivity, and their role in musculoskeletal hyperalgesia and inflammation.


Subject(s)
Acid Sensing Ion Channels/metabolism , Muscle, Skeletal/metabolism , Musculoskeletal Pain/metabolism , Myositis/metabolism , Nociception , Pain Threshold , Animals , Disease Models, Animal , Humans , Hydrogen-Ion Concentration , Muscle, Skeletal/innervation , Musculoskeletal Pain/physiopathology , Myositis/physiopathology , Signal Transduction
4.
Physiol Behav ; 135: 168-73, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24952267

ABSTRACT

Uncoupling protein 2 (UCP2) is widely distributed throughout the body including the brain, adipose tissue and skeletal muscles. In contrast to UCP1, UCP2 does not influence resting body temperature and UCP2-deficient (-/-) mice have normal thermoregulatory responses to a single exposure to cold ambient temperatures. Instead, UCP2-deficient mice are more anxious, exhibit anhedonia and have higher circulating corticosterone than wild type mice. To test the possible role of UCP2 in depressive behavior we exposed UCP2-deficient and wild type mice to a cold (26°C) forced swim and simultaneously measured rectal temperatures during and after the swim. The time that UCP2-deficient mice spent immobile did not differ from wild type mice and all mice floated more on day 2. However, UCP2-deficient mice were more able to defend against the decrease in body temperature during a second daily swim at 26°C than wild type mice (area under the curve for wild type mice: 247.0±6.4; for UCP2-deficient mice: 284.4±3.8, P<0.0001, Student's t test). The improved thermoregulation of wild type mice during a second swim at 26°C correlated with their greater immobility whereas defense against the warmth during a swim at 41°C correlated better with greater immobility of UCP2-deficient mice. Together these data indicate that while the lack of UCP2 has no acute effect on body temperature, UCP2 may inhibit rapid improvements in defense against cold, in contrast to UCP1, whose main function is to promote thermogenesis.


Subject(s)
Body Temperature Regulation/physiology , Cold Temperature , Ion Channels/metabolism , Mitochondrial Proteins/metabolism , Muscle, Skeletal/metabolism , Thermogenesis/physiology , Animals , Body Temperature/physiology , Ion Channels/genetics , Mice , Mice, Knockout , Mitochondrial Proteins/genetics , Swimming , Uncoupling Protein 2
5.
Pharmacol Res ; 79: 21-7, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24200896

ABSTRACT

Blocking, desensitizing, or knocking out transient receptor potential vanilloid type 1 (TRPV1) receptors decreases immobility in the forced swim test, a measure of depressive behavior. We questioned whether enhancing TRPV1 activity promotes immobility in a fashion that is prevented by antidepressants. To test this we activated heat-sensitive TRPV1 receptors in mice by water that is warmer than body temperature (41 °C) or a low dose of resiniferatoxin (RTX). Water at 41 °C elicited less immobility than cooler water (26 °C), indicating that thermoregulatory sites do not contribute to immobility. Although a desensitizing regimen of RTX (3-5 injections of 0.1 mg/kg s.c.) decreased immobility during swims at 26 °C, it did not during swims at 41 °C. In contrast, low dose of RTX (0.02 mg/kg s.c.) enhanced immobility, but only during swims at 41 °C. Thus, activation of TRPV1 receptors, endogenously or exogenously, enhances immobility and these sites are activated by cold rather than warmth. Two distinct types of antidepressants, amitriptyline (10mg/kg i.p.) and ketamine (50 mg/kg i.p.), each inhibited the increase in immobility induced by the low dose of RTX, verifying its mediation by TRPV1 sites. When desensitization was limited to central populations using intrathecal injections of RTX (0.25 µg/kg i.t.), immobility was attenuated at both temperatures and the increase in immobility produced by the low dose of RTX was inhibited. This demonstrates a role for central TRPV1 receptors in depressive behavior, activated by conditions (cold stress) distinct from those that activate TRPV1 receptors along thermosensory afferents (heat).


Subject(s)
Behavior, Animal/physiology , Depression/physiopathology , Depression/psychology , Receptors, N-Methyl-D-Aspartate/physiology , TRPV Cation Channels/physiology , Amitriptyline/pharmacology , Animals , Antidepressive Agents/pharmacology , Body Temperature , Diterpenes/pharmacology , Hot Temperature , Male , Mice , Stress, Physiological , Stress, Psychological , Swimming , TRPV Cation Channels/agonists
6.
J Pain ; 14(12): 1629-41, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24188863

ABSTRACT

UNLABELLED: Inactivation of transient receptor potential vanilloid-1 (TRPV1) receptors is one approach to analgesic drug development. However, TRPV1 receptors exert different effects on each modality of pain. Because muscle pain is clinically important, we compared the effect of TRPV1 ligands on musculoskeletal nociception to that on thermal and tactile nociception. Injected parenterally, capsaicin had no effect on von Frey fiber responses (tactile) but induced a transient hypothermia and hyperalgesia in both the tail flick (thermal) and grip force (musculoskeletal) assays, presumably by its agonistic action at TRPV1 sites. In contrast, resiniferatoxin (RTX) produced a chronic (>58 days) thermal antinociception, consistent with its reported ability to desensitize TRPV1 sites. In the same mice, RTX produced a transient hypothermia (7 hours) and a protracted (28-day) musculoskeletal hyperalgesia in spite of a 35.5% reduction in TRPV1 receptor immunoreactivity in muscle afferents. Once musculoskeletal hyperalgesia subsided, mice were tolerant to the hyperalgesic effects of either capsaicin or RTX whereas tolerance to hypothermia did not develop until after 3 injections. Musculoskeletal hyperalgesia was prevented but not reversed by SB-366791, a TRPV1 antagonist, indicating that TRPV1 receptors initiate but do not maintain hyperalgesia. Injected intrathecally, RTX produced only a brief musculoskeletal hyperalgesia (2 days), after which mice were tolerant to this effect. PERSPECTIVE: The effect of TRPV1 receptors varies depending on modality and tissue type, such that RTX causes thermal antinociception, musculoskeletal hyperalgesia, and no effect on tactile nociception in healthy mice. Spinal TRPV1 receptors are a potential target for pain relief as they induce only a short musculoskeletal hyperalgesia followed by desensitization.


Subject(s)
Diterpenes/toxicity , Hyperalgesia/metabolism , Musculoskeletal Pain/metabolism , Neurotoxins/toxicity , Pain Measurement/methods , TRPV Cation Channels/metabolism , Animals , Female , Hyperalgesia/chemically induced , Mice , Musculoskeletal Pain/chemically induced , Pain Measurement/drug effects
7.
PLoS One ; 8(8): e70740, 2013.
Article in English | MEDLINE | ID: mdl-23976955

ABSTRACT

Selective estrogen receptor modulators (SERMs) are effective therapeutics that preserve favorable actions of estrogens on bone and act as antiestrogens in breast tissue, decreasing the risk of vertebral fractures and breast cancer, but their potential in neuroprotective and procognitive therapy is limited by: 1) an increased lifetime risk of thrombotic events; and 2) an attenuated response to estrogens with age, sometimes linked to endothelial nitric oxide synthase (eNOS) dysfunction. Herein, three 3(rd) generation SERMs with similar high affinity for estrogen receptors (ERα, ERß) were studied: desmethylarzoxifene (DMA), FDMA, and a novel NO-donating SERM (NO-DMA). Neuroprotection was studied in primary rat neurons exposed to oxygen glucose deprivation; reversal of cholinergic cognitive deficit was studied in mice in a behavioral model of memory; long term potentiation (LTP), underlying cognition, was measured in hippocampal slices from older 3×Tg Alzheimer's transgenic mice; vasodilation was measured in rat aortic strips; and anticoagulant activity was compared. Pharmacologic blockade of GPR30 and NOS; denudation of endothelium; measurement of NO; and genetic knockout of eNOS were used to probe mechanism. Comparison of the three chemical probes indicates key roles for GPR30 and eNOS in mediating therapeutic activity. Procognitive, vasodilator and anticoagulant activities of DMA were found to be eNOS dependent, while neuroprotection and restoration of LTP were both shown to be dependent upon GPR30, a G-protein coupled receptor mediating estrogenic function. Finally, the observation that an NO-SERM shows enhanced vasodilation and anticoagulant activity, while retaining the positive attributes of SERMs even in the presence of NOS dysfunction, indicates a potential therapeutic approach without the increased risk of thrombotic events.


Subject(s)
Anticoagulants/pharmacology , Neurons/drug effects , Neuroprotective Agents/pharmacology , Nitric Oxide Donors/pharmacology , Nootropic Agents/pharmacology , Piperidines/pharmacology , Selective Estrogen Receptor Modulators/pharmacology , Thiophenes/pharmacology , Animals , Cell Hypoxia , Estrogen Receptor alpha/metabolism , Estrogen Receptor beta/metabolism , Glucose/deficiency , Male , Memory/drug effects , Mice , Neurons/cytology , Neurons/metabolism , Nitric Oxide/metabolism , Nitric Oxide Synthase Type III/antagonists & inhibitors , Nitric Oxide Synthase Type III/metabolism , Primary Cell Culture , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Rats , Receptors, Estrogen , Receptors, G-Protein-Coupled/antagonists & inhibitors , Receptors, G-Protein-Coupled/metabolism , Vasodilation/drug effects
8.
Neuropharmacology ; 72: 29-37, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23624287

ABSTRACT

The exacerbation of musculoskeletal pain by stress in humans is modeled by the musculoskeletal hyperalgesia in rodents following a forced swim. We hypothesized that stress-sensitive corticotropin releasing factor (CRF) receptors and transient receptor vanilloid 1 (TRPV1) receptors are responsible for the swim stress-induced musculoskeletal hyperalgesia. We confirmed that a cold swim (26 °C) caused a transient, morphine-sensitive decrease in grip force responses reflecting musculoskeletal hyperalgesia in mice. Pretreatment with the CRF2 receptor antagonist astressin 2B, but not the CRF1 receptor antagonist NBI-35965, attenuated this hyperalgesia. Desensitizing the TRPV1 receptor centrally or peripherally using desensitizing doses of resiniferatoxin (RTX) failed to prevent the musculoskeletal hyperalgesia produced by cold swim. SB-366791, a TRPV1 antagonist, also failed to influence swim-induced hyperalgesia. Together these data indicate that swim stress-induced musculoskeletal hyperalgesia is mediated, in part, by CRF2 receptors but is independent of the TRPV1 receptor.


Subject(s)
Hyperalgesia/etiology , Hyperalgesia/metabolism , Musculoskeletal Pain/etiology , Musculoskeletal Pain/metabolism , Receptors, Corticotropin-Releasing Hormone/metabolism , TRPV Cation Channels/metabolism , Acenaphthenes/therapeutic use , Analgesics/therapeutic use , Analysis of Variance , Animals , Body Weight/drug effects , Cold Temperature/adverse effects , Disease Models, Animal , Diterpenes/therapeutic use , Female , Hyperalgesia/drug therapy , Mice , Morphine/therapeutic use , Muscle Strength/drug effects , Pain Measurement , Peptide Fragments/therapeutic use , Peptides, Cyclic/therapeutic use , Reaction Time/drug effects , Receptors, Corticotropin-Releasing Hormone/antagonists & inhibitors , Swimming/psychology , TRPV Cation Channels/antagonists & inhibitors
9.
ACS Med Chem Lett ; 2(9): 656-661, 2011 Sep 08.
Article in English | MEDLINE | ID: mdl-21927645

ABSTRACT

Hybrid nitrate drugs have been reported to provide NO bioactivity to ameliorate side effects or to provide ancillary therapeutic activity. Hybrid nitrate selective serotonin reuptake inhibitors (NO-SSRIs) were prepared to improve the therapeutic profile of this drug class. A synthetic strategy for use of a thiocarbamate linker was developed, which in the case of NO-fluoxetine facilitated hydrolysis to fluoxetine at pH 7.4 within 7 hours. In cell culture, NO-SSRIs were weak inhibitors of the serotonin transporter, however, in the forced swimming task (FST) in rats, NO-fluoxetine demonstrated classical antidepressant activity. Comparison of NO-fluoxetine, with fluoxetine, and an NO-chimera nitrate developed for Alzheimer's disease (GT-1061), was made in the step through passive avoidance (STPA) test of learning and memory in rats treated with scopolamine as an amnesic agent. Fluoxetine was inactive, whereas NO-fluoxetine and GT-1061 both restored long-term memory. GT-1061 also produced antidepressant behavior in FST. These data support the potential for NO-SSRIs to overcome the lag in onset of therapeutic action and provide co-therapy of neuropathologies concomitant with depression.

10.
ACS Chem Neurosci ; 2(5): 256-268, 2011 May 18.
Article in English | MEDLINE | ID: mdl-21731800

ABSTRACT

The clinical benzothiophene SERM (BT-SERM), raloxifene, was compared with estrogens in protection of primary rat neurons against oxygen-glucose deprivation (OGD). Structure-activity relationships for neuroprotection were determined for a family of BT-SERMs displaying a spectrum of ERα and ERß binding affinity and agonist/antagonist activity, leading to discovery of a neuroprotective pharmacophore, present in the clinically relevant SERMS, raloxifene and desmethylarzoxifene (DMA), for which submicromolar potency was observed for neuroprotection. BT-SERM neuroprotection did not correlate with binding to ER nor classical ER activity, however, both the neuroprotective SERMs and estrogens were shown, using pharmacological probes, to activate the same kinase signaling cascades. The antiestrogen ICI 182,780 inhibited the actions of estrogens, but not those of BT-SERMs, whereas antagonism of the G-protein coupled receptor, GPR30, was effective for both SERMs and estrogens. Since SERMs have antioxidant activity, ER-independent mechanisms were studied using the classical phenolic antioxidants, BHT and Trolox, and the Nrf2-dependent cytoprotective electrophile, sulforaphane. However, neuroprotection by these agents was not sensitive to GPR30 antagonism. Collectively, these data indicate that the activity of neuroprotective BT-SERMs is GPR30-dependent and ER-independent and not mediated by antioxidant effects. Comparison of novel BT-SERM derivatives and analogs identified a neuroprotective pharmacophore of potential use in design of novel neuroprotective agents with a spectrum of ER activity.

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