Extraction behaviors of aqueous PEG impregnated resin system in terms of impregnation stability and recovery via protein impregnated resin interactions on bovine serum albumin.

BACKGROUND: Current advances in biotechnology have been looked at as alternative approaches towards the limited product recovery due to time- and cost-consuming drawbacks on the conventional purification methods. This study aimed to purify bovine serum albumin (BSA) as an exemplary target product using an aqueous impregnated resin system (AIRS). This method implies the concept of hydrophobicity of polymer that impregnated into the resins and driven by electrostatic attractions and hydrophilicity of aqueous salt solution to extract the target product. METHODS: The extraction behaviors of impregnation in terms of stability and adsorption kinetics via protein-aqueous polymer impregnated resin were studied. Impregnation stability was determined by the leaching factor of polyethylene glycol (PEG). The major factors such as PEG molecular weights and concentration, pH of aqueous salt solution, extraction methods (sonication and agitation) and types of adsorbent material and concentration of aqueous salt phase influencing on partitioning of biomolecule were also investigated. RESULTS: For impregnation stability, the leaching factor for Amberlite XAD4 did not exceed 1%. The scanning electron microscopy (SEM) image analysis of Amberlite XAD4 attributes the structural changes with impregnation of resins. For adsorption kinetics, Freundlich adsorption isotherm with the highest R2 value (0.95) gives an indication of favorable adsorption process. Performance of AIRS impregnated with 40% (w/w) of PEG 2000 was found better than aqueous-two phase system (ATPS) by yielding the highest recovery of BSA (53.72%). The outcomes of this study propound the scope for the application of AIRS in purification of biomolecules.

Recovery of a Bacteriocin-Like Inhibitory Substance from Lactobacillus bulgaricus FTDC 1211 Using Polyethylene-Glycol Impregnated Amberlite XAD-4 Resins System.

Lactobacillus bulgaricus is a LAB strain which is capable of producing bacteriocin substances to inhibit Staphylococcus aureus. The aim of this study was to purify a bacteriocin-like inhibitory substance (BLIS) produced by L. bulgaricus FTDC 1211 using an aqueous impregnated resins system consisting of polyethylene-glycol (PEG) impregnated on Amberlite XAD4. Important parameters influencing on purification of BLIS, such as the molecular weight and concentration of PEG, the concentration and pH of sodium citrate and the concentration of sodium chloride, were optimized using a response surface methodology. Under optimum conditions of 11% (w/w) of PEG 4000 impregnated Amberlite XAD4 resins and 2% (w/w) of sodium citrate at pH 6, the maximum purification factor (3.26) and recovery yield (82.69% ± 0.06) were obtained. These results demonstrate that AIRS could be used as an alternate purification system in the primary recovery step.