Unknown spa types, spa repeats, and relatedness of MRSA isolated from horses, dogs, cats, and their human handlers.

Methicillin resistant Staphylococcus aureus (MRSA) represents a worrying example of antimicrobial resistance, and it is essential to acquire new information to monitor the spread and limit it further diffusion. This study aimed to characterise 22 MRSA isolates from horses, dogs, cats, and their human handlers focusing on spa typing. In the analysis of the sequences obtained, the spa type is "unknown" (unidentified) and all the sequences except one had repeats previously not known in all databases potentially indicating new spa-repeats. This could possibly indicate either permanent import of novel spa types or in-house microevolution of spa repeats.

Characterisation and prevalence of community-associated MRSA among horses, dogs, cats and their human handlers: a cross-sectional study.

Methicillin-resistant Staphylococcus aureus (MRSA) as an infectious organism of public health significance has evolved to a genetically distinct community-acquired MRSA with extended resistance to other than ß-lactams. A cross-sectional study was conducted among 149 participants handling 446 animals (240 horses and 206 companion animals). The isolates were characterised as S. aureus and MRSA based on polymerase chain reaction detection of the nuc, mecA and mecC genes and the pvl gene for differentiation as community associated/livestock associated or hospital associated. The isolation rate of S. aureus from the human handlers' samples was 26 (17.4%) and 170 (38.1%) from the animal samples. The prevalence of MRSA among the isolates was 7 (4.7%) from the human handlers and 19 (4.3%) from the animals. Dogs and dog handlers had the highest isolation rates and were more likely to be colonized by S. aureus and MRSA compared with horses, cats and their handlers. The highest prevalence of MRSA was from horses (5.0%) and dog handlers (10.6%). This study has demonstrated a high prevalence of community associated MRSA in apparently healthy animals and their human handlers. This has important implications for antibiotic selection and use as well as infection control measures.