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1.
Dokl Biochem Biophys ; 476(1): 299-302, 2017 Sep.
Article in English | MEDLINE | ID: mdl-29101743

ABSTRACT

We found a two-fold increase in the productivity of baker's yeast grown on a nutrient mixture prepared in light water with a D2O content (127 ppm) smaller than in the distilled water (150 ppm). The number of water monomers that provides the biosynthetic activity (water transport through membrane channels) of yeast cells with an increased CO2 yield was determined for the first time. We established that the selectivity of cell membrane channels in water of different composition depends not only on the motion of ortho-and para-spin H2O isomers in solution, as was shown earlier, but also on the concentration of D2O.


Subject(s)
Deuterium Oxide/metabolism , Saccharomyces cerevisiae/metabolism , Carbon Dioxide/metabolism , Fermentation , Kinetics
2.
Biofizika ; 59(2): 408-13, 2014.
Article in Russian | MEDLINE | ID: mdl-25702495

ABSTRACT

It has been experimentally established that cultivation of yeast cells in depleted, dietary or normal nutrient aqueous solutions enriched with ortho-H2O spin isomers is accompanied by an increase in the amount of carbon dioxide produced by the cells and an increase in their biomass. It has been revealed that the rate of metabolic processes and biological activity depends on the quality of nutrition and enhances in time in both nutrient solutions. In contrast, the reproductive function and the rate of cell division are insusceptible to the components of nutrition, but intensified in a solution enriched with ortho-H2O similar to retardation of aging. The observed effects are discussed in assumption that an increase of a portion of ortho-H2O molecules occurs in the neighborhood of water channels in the cell membrane that let through only monomers of H2O and determine the rate of metabolic processes.


Subject(s)
Saccharomyces cerevisiae/metabolism , Water/chemistry , Isomerism , Saccharomyces cerevisiae/growth & development , Water/metabolism
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