ABSTRACT
An endophytic bacterium, MA-69T, was isolated from the storage liquid in the stems of Populuseuphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain MA-69T was found to be short rod-shaped, Gram-stain-negative, non-spore-forming, aerobic and motile by means of a monopolar flagellum. According to phylogenetic analysis based on 16S rRNA gene sequences, strain MA-69T was assigned to the genus Pseudomonas with highest 16S rRNA gene sequence similarity of 97.5â% to Pseudomonas azotifigens JCM 12708T, followed by Pseudomonas matsuisoli JCM 30078T (97.5â%), Pseudomonas balearica DSM 6083T (97.1â%), Azotobacter salinestris ATCC 49674T (96.1â%) and Pseudomonas indica DSM 14015T (95.9â%). Analysis of strain MA-69T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the isolate to be distinctly delineated from species of the genus Pseudomonas. The DNA G+C content of strain MA-69T was 64.1 mol%. DNA-DNA hybridization with Pseudomonas azotifigens JCM 12708T, Pseudomonas matsuisoli JCM 30078T and Pseudomonas balearica DSM 6083T revealed 62.9, 60.1 and 49.0â% relatedness, respectively. The major fatty acids in strain MA-69T were summed feature 3 (25.7â%), summed feature 8 (24.0â%), C19â:â0cyclo ω8c (19.9â%), C16â:â0 (14.6â%) and C12â:â0 (6.3â%). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Q-9 was the major quinone in strain MA-69T. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain MA-69T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas tarimensis sp. nov. is proposed. The type strain is MA-69T (=CCTCC AB 2013065T=KCTC 42447T).
