Mesozooplankton communities related to water masses in the Persian Gulf and the Gulf of Oman.

The spatial and temporal variations of mesozooplankton in relation to environmental parameters were investigated in the Persian Gulf and the Gulf of Oman. For this purpose, the physicochemical parameters were measured using a CTD probe and mesozooplankton were sampled vertically using a closing net aboard the RV Persian Gulf Explorer during two expeditions. Results showed a relationship between mesozooplankton assemblages and water mass distributions. Four mesozooplankton cluster station groups divided the sampled stations into masses, which was also confirmed by the PERMANOVA test. Overall, ninety taxa were identified that were dominated by copepods and characterized by small cyclopoids. Based on the results the mesozooplankton communities of the Persian Gulf and the Gulf of Oman are mostly shaped by the combined effect of temperature, salinity, and dissolved oxygen that significantly influences mesozooplankton occurrence during summer and spring.

Mesozooplankton community in near-hypoxic and hypoxic layers of the Persian Gulf and the Gulf of Oman.

To assess the role of near-hypoxic and hypoxic conditions on the mesozooplankton community, we studied twenty stations in the Persian Gulf and the Gulf of Oman. The physicochemical parameters were measured using a CTD probe and mesozooplankton were sampled vertically using a closing net. Results showed the hypoxic conditions of 0.52 ± 0.11, 1.40 ± 0.48, and 0.84 ± 0.08 in the lower layers in stations T11S3, T0S8B, and T9S5 in the Gulf of Oman and near-hypoxic conditions in station T6S3 (2.99 ± 0.05) in the Persian Gulf. The marginal test revealed that stratification during summer and dissolved oxygen and salinity during spring in the Persian Gulf and temperature, stratification, and dissolved oxygen during spring in the Gulf of Oman significantly explained the variation of mesozooplankton composition in the study areas (p < 0.05). The present study reveals some general patterns of mesozooplankton assemblage in near-hypoxic and hypoxic conditions, but a future detailed investigation will be essential.

Development and characterization of a thermostable GH11/GH10 xylan degrading chimeric enzyme.

Xylanases are categorized into different family groups, two of which are glycoside hydrolases 10 (GH10) and 11 (GH11) families. These well-characterized xylanases demonstrate different modes of action in hydrolysis of xylans. Imitating certain types of microorganisms to produce bifunctional enzymes such as engineered xylanases has gained considerable attention among researchers. In this study, a recombinant chimeric enzyme (X11-10) was designed by fusing two thermostable xylanases through a peptide linker. The recombinant parental enzymes, xylanase 10 from fungus Bispora sp. MEY-1 (X10) and xylanase 11 from bacterium Thermobacillus xylanilyticus (X11), and their chimera were successfully expressed in Pichia pastoris (P. pastoris), purified, and characterized. Being active over a wide pH range, X11-10 chimera showed higher thermal stability, possessed a lower Km, and a higher catalytic efficiency (kcat/Km) in comparison to the parental enzymes. Also, molecular dynamics simulation (MDS) of X11-10 revealed that its active site residues were free to interact with substrate. This novel chimeric xylanase may have potential applications in different industrial processes since it can substitute two separate enzymes and therefore minimize the production costs.

Hypoxia in the Persian Gulf and the Strait of Hormuz.

In this article dissolved oxygen results of research cruises through the Persian Gulf during 2018-2019 are discussed. The results showed that summer to autumn hypoxia occurred mainly at depths ≥ 50 m to the bottom. This seasonal hypoxia started in late summer reaching its greatest severity in mid-autumn with an area of 50,000 km2 in the Persian Gulf. The minimum oxygen measured at the near-bottom layer of the western basin in autumn (25.8 µmol/kg) was lower than any previous measurement in the open waters of the Persian Gulf. In the Strait of Hormuz, the seasonal hypoxia appeared in summer in the near bottom of the most eastern part at the Iranian side. pHT values recorded in hypoxic waters were as low as what is predicted for surface ocean under ocean acidification in 2100. Considering the results, we suggest evaluating the effects of hypoxia and acidification on the Persian Gulf ecosystems.

Concentration of polycyclic aromatic hydrocarbons in zooplanktons of Bushehr coastal waters (north of the Persian Gulf).

In this study, polycyclic aromatic hydrocarbon contents in zooplanktons of coastal waters of Bushehr Province at the north of the Persian Gulf were evaluated. Zooplankton samples were collected from 18 stations in both summer and winter (2017), and their polycyclic aromatic hydrocarbon contents were measured by gas chromatography-mass spectrometry after ultrasound-assisted extraction. Results revealed that among 16 compounds that were analyzed, only 7 compounds were recognizable at both seasons. Pyrene was the most common individual compound of polycyclic aromatic hydrocarbons, whereas acenaphthene was the least individual compound found at both seasons. Additionally, the highest content of the measured compounds in both summer and winter was 16.4-75.9 ng/g and 17.3-93.7 ng/g, respectively. On the basis of the obtained results, the total concentration of polycyclic aromatic hydrocarbons was significantly higher in winter than in summer (P < 0.05).

Trace metals in zooplankton from the northern Persian Gulf.

This work is the first study on the spatial and temporal distribution of metals in zooplankton collected along the Persian Gulf. The rank order distribution of metals in terms of their overall average concentration in the zooplankton was observed to be Fe > Zn > Cu > Cd > Ni > Cr > Pb > Co > V. No clear pattern and no statistical differences were detected among the areas and the sampling stations. The highest concentrations of Cu, Zn, and Pb were observed in summer while the highest concentration levels of Fe and Ni were observed in winter. The highest mean zooplankton metal concentration level was found to be Fe with 10.42 µg/g (in winter) and the lowest levels were for V (in spring and summer) and Co (in winter), both of which had a level of 0.01 µg/g. The results can be used to establish base-line data for the trace metal content of zooplankton in the northern region of the Persian Gulf.

Data on diversity and abundance of zooplanktons along the northern part of the Persian Gulf, Iran.

In this data article, we aimed to evaluate and compare the biological diversity and relative abundance of zooplankton communities in 3 different areas along the northern part of the Persian Gulf in 3 different seasons. Data showed that Centropages spp and Fish larvae were the highest and lowest species among the groups identified in summer in Lavare Saheli and Nakhle Taghi with relative abundances of 87% and 2.7% respectively. In winter, Cyphonautes larvae and Corycaeus spp. were the highest and lowest species in Kangan and Lavare Saheli with relative abundances of 57.1% and 1.88%, respectively. Also Decapoda larvae spp. and Gastropoda larvae were the highest and lowest species in spring in Kangan with relative abundances of 62.5% and 4.7% respectively. Data may serve as benchmarks for other groups working in the field of pollution control, aquatic ecosystem, and toxicology.

Potential of the green alga Chlorella vulgaris for biodegradation of crude oil hydrocarbons.

Oil production and/or transportation can cause severe environmental pollution and disrupt the populations of living organisms. In the present study, biodegradation of petroleum hydrocarbons is investigated using Chlorella vulgaris as a green algal species. The microalga was treated by 10 and 20g/l crude oil/water concentrations at two experimental durations (7 and 14days). Based on the results obtained, C. vulgaris owned not only considerable resistance against the pollutants but also high ability in remediation of crude oil hydrocarbons (~94% of the light and ~88% of heavy compounds in 14days). Intriguingly, dry weight of C. vulgaris increased by the rising crude oil concentration indicating the positive effect of crude oil on the growth of the algal species. This biodegradation process is remarkably a continuous progression over a period of time.

Biodiesel production in crude oil contaminated environment using Chlorella vulgaris.

Biodiesel is a valuable alternative to fossil fuels and many countries choose biodiesel as an unconventional energy source. A large number of investigations have been done on microalgae as a source of oil production. In recent years, wastewater pollutions have caused many ecological problems, and therefore, wastewater phycoremediation has attracted the international attention. This paper studied the cultivation of Chlorella vulgaris in a crude oil polluted environment for biodiesel production. Intended concentrations were 10 and 20gperliter (crude oil/water) at two times. The results showed that the growth of C. vulgaris was improved in wastewater and the maximum amount of dry mass and oil was produced at the highest concentration of crude oil (0.41g and 0.15g/l, respectively). In addition, dry mass and oil yield of the microalga were significantly enhanced by increasing the experiment duration.

Antifouling paint booster biocides (Irgarol 1051 and diuron) in marinas and ports of Bushehr, Persian Gulf.

In the present study, antifouling paint booster biocides, Irgarol 1051 and diuron were measured in ports and marinas of Bushehr, Iran. Results showed that in seawater samples taken from ports and marinas, Irgarol was found at the range of less than LOD to 63.4ngL(-1) and diuron was found to be at the range of less than LOD to 29.1ngL(-1) (in Jalali marina). 3,4-dichloroaniline (3,4-DCA), as a degradation product of diuron, was also analyzed and its maximum concentration was 390ngL(-1). Results for analysis of Irgarol 1051 in sediments showed a maximum concentration of 35.4ngg(-1) dry weight in Bandargah marina. A comparison between the results of this study and those of other published works showed that Irgarol and diuron pollutions in ports and marinas of Bushehr located in the Persian Gulf were less than the average of reports from other parts of the world.

Source identification of polycyclic aromatic hydrocarbons (PAHs) in sediment samples from the northern part of the Persian Gulf, Iran.

Samples of surface sediments from the Iranian coast of the Persian Gulf were examined to determine the levels and sources of 15 polycyclic aromatic hydrocarbons (PAHs). Samples were collected from 30 sampling sites and analyzed for PAHs by gas chromatography-mass spectrometry (GC-MS). Total concentrations of PAHs ranged from 93 to 4,077 ng g(-1) dry weight. The PAH composition from 30 sampling sites was dominated by four-ring PAH compounds. Molecular indices based on the ratios of PAH concentrations were used to differentiate PAHs from pyrolitic to petrogenic and mixed origins. The results suggested that the main sources of PAHs in sediments from the studied region were mixed pyrolitic and petrogenic. Furthermore, benthic organisms in most of the investigated areas were not at ecotoxicological risk, according to the results from the effects range low (ERL)/effects range median (ERM) techniques suggested by the US Sediment Quality Guidelines (SQGs).

The role of urinary TIMP1 and MMP9 levels in predicting vesicoureteral reflux in neonates with antenatal hydronephrosis.

BACKGROUND: Antenatal hydronephrosis (AH) is commonly found on evaluation of pregnant women and 20-30 % of neonates have vesicoureteral reflux (VUR). In order to diagnose VUR, we required invasive testing and exposure of the neonate to radiation. The concentrations of a matrix metalloproteinase, MMP9, and its inhibitor TIMP1, were analyzed in hydronephrotic newborns with VUR and were compared to those without reflux. METHODS: The neonates with a history of prenatal hydronephrosis were enrolled in two groups based on imaging study results, the neonates with VUR and without VUR. Neonates with a normal prenatal history and postnatal ultrasound were placed in a third group. We measured the random urinary levels of MMP9, TIMP1, and creatinine, their cut-off values and the MMP9/Cr and MMP9/TIMP1/Cr ratio was calculated, and an ROC curve was drawn. RESULTS: Sixty-nine neonates were enrolled in three groups; 27 patients (20 male, seven female) with AH and VUR were in group 1, 23 neonates (19 male, four female) without VUR were placed in group 2, and 19 (15 male, four female) acted as controls in group 3. The differences between the three groups and the normal and total hydronephrotic groups were statistically significant for MMP9, the MMP9/Cr, MMP9/TIMP1, and MMP9/TIMP1/Cr ratios. The urinary TIMP1 and TIMP1/Cr ratios were not significantly different between the groups. A cut-off value of MMP9 was measured as 358.5 ng/ml (sensitivity [sens] 74 %, specificity [spec] 78 %) and was used to compare groups 1 and 2. For groups 2 and 3, this cut-off was 181.00 pg/ml (sens 91 %, spec 89 %). The cut-off values measured for the MMP9/TIMP1 ratio were 30.32 (sens 70 %, spec 61 %) and 9.85 (sens 96 %, spec 89 %) to compare groups 1 and 2, and 2 and 3, respectively. We found no valuable cut-offs for the TIMP1 and TIMP1/Cr values. There was no difference between neonates with mild, moderate, and severe VUR according to urinary biomarker concentrations. CONCLUSIONS: Evaluation of urinary levels of MMP9, or the MMP9/Cr, MMP9/TIMP1, or MMP9/TIMP1/Cr ratios may help us to differentiate the newborns with hydronephrosis and VUR from those without reflux.