Ethnoveterinary uses of forage plants for domesticated ruminants in Malakand agency, Pakistan.

Plant ethnoveterinary uses are evident in various studies around the world, but the ethnoveterinary practices of forage species are not widely reported. Traditional knowledge is rapidly disappearing because of urbanization and commercial activities. The purpose of this study was to document plant species used by the local communities in Malakand Agency, Pakistan for foraging and ethnoveterinary purposes. Twenty different localities in the study area were surveyed for documentation of forage species and related traditional ethnoveterinary knowledge used for livestock. Semistructured questionnaires and field walks were used to conduct 67 interviews with local farmers and herdsmen. A total of 91 forage species from 26 families were documented, as well as their ethnoveterinary applications. Poaceae and Fabaceae were dominant families with 45% and 15% of species respectively. Among the forage species documented, 62 were highly palatable, 26 were moderately palatable and 12 were less palatable. The region's major veterinary diseases are flu, ringworms, inflammations, low milk production, constipation, bloat, mastitis, pneumonia and wounds. The 62 forage species were reported for the first time for various veterinary uses. This study revealed that local communities commonly use a diverse range of forage species in conjunction with indigenous knowledge of ethnoveterinary uses. These forage species have the potential to overcome the recent fodder shortage. Such studies will be beneficial to the commercial production of such forage species.

Palyno-anatomical microscopic characterization of selected species of Boraginaceae and Fabaceae.

Palyno-anatomical characterization of some selected species of Boraginaceae and Fabaceae using light microscopy (LM) and scanning electron microscopy (SEM) was conducted. Six species of them belongs to four genera of family Boraginaceae and other six species belongs to four genera of Fabaceae. The current study aimed to investigate palyno-anatomical features for correct identification and taxonomic significance. Pollen grains were acetolyzed and observed both qualitatively and quantitatively through light microscopy and scanning electron microscopy. Statistical data was analyzed through cluster analysis which categorized plants into clusters. Principal component analysis (PCA) was carried out to illustrate morphological variation among selected plant species. Studied plant species showed variation in shape, size, aperture type, exine ornamentation of pollen, size and shape of epidermal cells, types of stomata, guard cells, subsidiary cells and trichome types. Exine thickness was recorded maximum 2.30 ± 0.1 µm in Lablab purpureus and minimum 1.05 ± 0.2 in Gastrocotyle hispida. PCA summarized 73.78% of accumulative variance. Trichome index was observed highest 62% on the adaxial surface of Heliotropium europaeum and lowest 21% on adaxial surface of L. purpureus. These findings reinforce the importance of palyno-anatomical features in the characterization and identification of taxa. It was concluded that description of palyno-anatomical characteristics presented in this study, highly contribute to our knowledge for correct identification of plant species.

Detailed macro and micromorphology of grasses caryopsis using scanning electron and light microscopy.

Micromorphology of 15 grass caryopsis was investigated through scanning electron microscopy for its implications in the identification of grasses. Both macro and micromorphological characters especially caryopsis shape and surface morphology were found significant in the taxonomic diagnosis. Shape morphology of caryopsis varied among the species and lanceolate, fusiform, narrow ellipsoidal, ovate, elliptical, plano-convex, fusiform, linear ovate, broadly elliptical, broadly pllipsoidal, plano-convex, oblique ovate, and subglobose shapes were recorded. Digitaria nodosa (3.31 × 0.7 mm) and Echinochloa crus-galli (3.26 × 1.44 mm) had the largest caryopsis size, while Acrachne racemosa (0.76 × 0.54 mm) and Eragrostis minor (0.62 × 0.38 mm) had the smallest caryopsis size. Macro and micromorphological characters were useful for the diagnosis of genus and species. Utilizing both qualitative and quantitative character taxonomic key at generic and species level were made.

Ethnoveterinary botanical survey of medicinal plants used in Pashto, Punjabi and Saraiki communities of Southwest Pakistan.

Medicinal plants are highly used in the ethnoveterinary practice as considerable livestock resources in remote areas. The aim of the present study is to explore the ethnoveterinary medicinal practices in three different communities and discuss the cross-cultural consensus on the usage of medicinal plants for the treatment of animals. The field survey was conducted by the animal healers of the area during the different seasons of plant growth. A total of 83 informants were interviewed through Semi-structured interview involving experts of traditional knowledge in 21 localities of the three regions (Zhob, D. I. Khan and Mianwali) were conducted. Findings of the study were quantitatively analyzed through the informant consensus factors to identify the homogeneity information provided by the informants. Furthermore, cross-culture consensuses were analyzed and recorded data were represented in a tabulated and Venn diagrams. In particularly, 59 species of plants were documented in the comparative analysis. Among them, 32 plant species were recorded in Pashto community, while Punjabi and Sarakai communities exhibited nine and four plant species, respectively. Whereas cross-cultural analysis showed 14 medicinal plants that were commonly utilized by three different ethnic communities, that indicated low interregional consensus in regard to ethnoveterinary practices of medicinal plants. The current study showed that different communities and ethnic groups sharing some traditional knowledge and cross-culturally approaches have been reported from traditional uses of plants against livestock's diseases. Therefore, current findings are the opportunities to scrutinize the plants for the discovery of new drug sources for humans and animals.

Antimicrobial and antioxidant chlorinated azaphilones from mangrove Diaporthe perseae sp. isolated from the stem of Chinese mangrove Pongamia pinnata.

Many plants remain unexplored for their endophytic fungi that may possess potentially important phytochemicals. Consequently, we have focused to discover new natural products from endophytic fungus Diaporthe perseae sp. isolated from the stem of the Chinese mangrove Pongamia pinnata (L.) Pierre plant that led to the isolation of one new chlorinated isochromophilone G (1) along with six known azaphilones (2-7). The structures of the isolated compounds were elucidated by UV, NMR and Mass spectroscopic analysis. All the isolated compounds were screened for their antimicrobial and anti-oxidant activities.

Utilization of foliar cuticle morphology for the identification of weedy grasses.

For the robust identification of weeds of taxonomically complex families like Poaceae, scanning electron microscopy (SEM) and light microscopy (LM) are of significant implications. In this study, SEM and LM methods were used by investigating 12 Pakistani weedy grass species as an example for foliar epidermal micromorphological characters for its role in the identification of grasses species. Qualitative and quantitative foliar micromorphological variations in stomatal type, number, guard cells, subsidiary cells, silica bodies and trichomes were studied. Significant variation was observed in both qualitative and quantitative features and based on this diversity in epidermal characters, a key was drawn to delimitate the species. Variations in stomata, silica bodies (SiO2 ·nH2 O), macrohairs, microhairs, hooks, papillae, prickles, and other epidermal structures were used in establishing the taxonomic key. The data were also statistically evaluated by determining its mean, standard deviation, variance, the coefficient of variance, and standard error. Foliar epidermal characters such as silica bodies shape and trichomes were taxonomically more useful than stomatal types, subsidiary, and guard cells. SEM and LM micromorphological characters were of substantial importance in the delimitation of closely related species.

Micro-morphological, environmental and phytochemical investigation of herbal medicines by using LM and SEM: A quality control tool.

The use of quality control tool for authentication of Jadwar (Delphinium denudatum Wall. ex Hook.f. & Thomson), a folk herbal drug used for the treatment of different ailments, was studied. People face problems of adulteration for this drug at global, regional, national and local levels. Two different plant species are commercially marketed in the Indo-Pak Subcontinent under the same trade name of Jadwar. One is D. denudatum Wall. ex Hook.f. & Thomson and the other is Aconitum heterophyllum Wall. ex Royle. To focus on this problem, a marketable available drug sample of Jadwar was authenticated by using basic microscopy tools (LM) and advanced chemo-taxonomic markers. Authentication, quality and standardization of this drug was achieved using morphology, organoleptography, UV and IR analyses, scanning electron microscopy of pollen and anatomical investigations. The techniques used for authentication marked the clear difference between the studied plants. Microscopic studies, chemotaxonomic investigation and other techniques used in this project provided the basis for the authentication of this species.