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1.
Molecules ; 23(7)2018 07 10.
Article in English | MEDLINE | ID: mdl-29996498

ABSTRACT

Essential oils (EOs) were obtained by hydrodistillation of various parts of Ferula ovina (Boiss.) Boiss., Ferula iliensis Krasn. ex. Korovin, and Ferula akitschkensis B. Fedtsch. ex Koso-Pol., collected in the flowering/budding and fruiting stages. Eight samples of EOs isolated from F. ovina and four samples from F. akitsckensis were analyzed by gas chromatography⁻mass spectrometry (GC-MS). The major constituents of F. ovina EOs were α-pinene (6.9⁻47.8%), ß-pinene (1.5⁻7.1%), sabinene (0.1⁻20.5%), ß-phellandrene (0⁻6.5%), trans-verbenol (0.9⁻7.4%), eremophilene (3.1⁻12%), and 6Z-2,5,5,10-tetramethyl-undeca-2,6,9-trien-8-one (0⁻13.7%). The major constituents of F. akitsckensis EOs were α-pinene (0⁻46.2%), ß-pinene (0⁻47.9%), sabinene (0⁻28.3%), eremophilene (0⁻10.6), ß-caryophyllene (0⁻7.5%), himachalen-7-ol (0⁻28.2%), and an himachalol derivative (0⁻8.3%). Samples of EOs from F. ovina, F. iliensis, and F. akitsckensis were evaluated for antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) pulse-field gel electrophoresis type USA300 (LAC). EOs from F. ovina exhibited the highest antibacterial activity compared to samples from other Ferula spp., with the most potent EOs being isolated from roots at the flowering and fruiting stages and stems at the fruiting stage (IC50 values of 19.1, 20.9, and 22.9 µg/mL, respectively). Although EOs demonstrated concentration-dependent inhibition of MRSA growth, analysis of the major constituents (α-pinene, ß-pinene, and sabinene) showed that they had low activity, suggesting that other components were likely responsible for the observed bioactivity of the unfractionated EOs. Indeed, correlation of the GC-MS data with antibacterial activity suggested that the putative components responsible for antibacterial activity were, either individually or in combination, eremophilene and trans-verbenol. Overall, these results suggest that the EOs from F. ovina could have potential for use as alternative remedies for the treatment of infectious diseases caused by MRSA.


Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Ferula/chemistry , Methicillin-Resistant Staphylococcus aureus/drug effects , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Linear Models , Microbial Sensitivity Tests
2.
J Leukoc Biol ; 101(6): 1361-1371, 2017 06.
Article in English | MEDLINE | ID: mdl-28258152

ABSTRACT

Essential oil extracts from Ferula iliensis have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were (E)-propenyl sec-butyl disulfide (15.7-39.4%) and (Z)-propenyl sec-butyl disulfide (23.4-45.0%). Ferula essential oils stimulated [Ca2+]i mobilization in human neutrophils and activated ROS production in human neutrophils and murine bone marrow phagocytes. Activation of human neutrophil [Ca2+]i flux by Ferula essential oils was dose-dependently inhibited by capsazepine, a TRPV1 channel antagonist, indicating that TRPV1 channels mediate this response. Furthermore, Ferula essential oils stimulated Ca2+ influx in TRPV1 channel-transfected HEK293 cells and desensitized the capsaicin-induced response in these cells. Additional molecular modeling with known TRPV1 channel agonists suggested that the active component is likely to be (Z)-propenyl sec-butyl disulfide. Our results provide a cellular and molecular basis to explain at least part of the beneficial therapeutic properties of FEOs.


Subject(s)
Ferula/chemistry , Neutrophils/immunology , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Phagocytes/immunology , Animals , Calcium/metabolism , Cells, Cultured , HEK293 Cells , Humans , Mice , Neutrophils/drug effects , Neutrophils/metabolism , Phagocytes/drug effects , Phagocytes/metabolism , TRPV Cation Channels/metabolism
3.
J Agric Food Chem ; 64(38): 7156-70, 2016 Sep 28.
Article in English | MEDLINE | ID: mdl-27586050

ABSTRACT

Essential oils were obtained by hydrodistillation of the umbels+seeds and stems of Ferula akitschkensis (FAEOu/s and FAEOstm, respectively) and analyzed by gas chromatography and gas chromatography-mass spectrometry. Fifty-two compounds were identified in FAEOu/s; the primary components were sabinene, α-pinene, ß-pinene, terpinen-4-ol, eremophilene, and 2-himachalen-7-ol, whereas the primary components of FAEOstm were myristicin and geranylacetone. FAEOu/s, ß-pinene, sabinene, γ-terpinene, geranylacetone, isobornyl acetate, and (E)-2-nonenal stimulated [Ca(2+)]i mobilization in human neutrophils, with the most potent being geranylacetone (EC50 = 7.6 ± 1.9 µM) and isobornyl acetate 6.4 ± 1.7 (EC50 = 7.6 ± 1.9 µM). In addition, treatment of neutrophils with ß-pinene, sabinene, γ-terpinene, geranylacetone, and isobornyl acetate desensitized the cells to N-formyl-Met-Leu-Phe (fMLF)- and interleukin-8 (IL-8)-induced [Ca(2+)]i flux and inhibited fMLF-induced chemotaxis. The effects of ß-pinene, sabinene, γ-terpinene, geranylacetone, and isobornyl acetate on neutrophil [Ca(2+)]i flux were inhibited by transient receptor potential (TRP) channel blockers. Furthermore, the most potent compound, geranylacetone, activated Ca(2+) influx in TRPV1-transfected HEK293 cells. In contrast, myristicin inhibited neutrophil [Ca(2+)]i flux stimulated by fMLF and IL-8 and inhibited capsaicin-induced Ca(2+) influx in TRPV1-transfected HEK293 cells. These findings, as well as pharmacophore modeling of TRP agonists, suggest that geranylacetone is a TRPV1 agonist, whereas myristicin is a TRPV1 antagonist. Thus, at least part of the medicinal properties of Ferula essential oils may be due to modulatory effects on TRP channels.


Subject(s)
Ferula/chemistry , Immunologic Factors/pharmacology , Neutrophils/drug effects , Oils, Volatile/pharmacology , Plant Oils/pharmacology , Aldehydes/pharmacology , Camphanes/pharmacology , Capsaicin/pharmacology , Cell Movement/drug effects , Gas Chromatography-Mass Spectrometry , HEK293 Cells , HL-60 Cells , Humans , Interleukin-8/metabolism , N-Formylmethionine Leucyl-Phenylalanine/analogs & derivatives , N-Formylmethionine Leucyl-Phenylalanine/metabolism , Neutrophils/metabolism , Oils, Volatile/chemistry , Plant Oils/chemistry , Seeds/chemistry , TRPV Cation Channels/metabolism , Terpenes/pharmacology , Transient Receptor Potential Channels/metabolism
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