ABSTRACT
The aim of the article was to study the effect of electromagnetic radiation on toxicity of Vipera lebetina obtusa venom. It was found that mice intoxicated with snake venom, with moderate to high exposure to electromagnetic radiation and mice intoxicated with venom, which had not been exposed to the radiation showed the same symptoms of intoxication and death. At the same time, the longevity of mice intoxicated with venom exposed to electromagnetic radiation was higher. The longevity of mice in control group was 25+/-5 min. The longevity of mice intoxicated with exposed to electromagnetic radiation snake venom was from 29 to 60 min. The research showed that the longevity of mice intoxicated with snake venom rose with the level of electromagnetic radiation intensity the snake was exposed to. Accordingly, snake venom, with exposure to high intensity electromagnetic radiation is less toxic.
Subject(s)
Radiation , Snake Venoms/toxicity , Snakes , Animals , Injections, Intraperitoneal , Mice , Snake Venoms/administration & dosageABSTRACT
The aim of the investigation was to work out the atom-absorption spectrometry method for definition of elementary structure of snake venom. The investigation was conducted on venom vipers, caught in the Gobustan district. Determination of heavy metals in venom vipers, was conducted by means of atom-absorption spectrometry (Perkin-Elmer AAS-300). The concentration of the following metals in samples of Vipera Lebetina obtusa venom was defined: Cd (0,012%), (1,234%), Fe (0,487%), Cr (0,171%), Zn (0,78%). The obtained data by means of proposed method of definition of heavy metals in samples of snake venom can be applied to standardize the snake venom, to establish the authenticity in a forensic medical examination, in analyses of medication on the basis of snake venom.
Subject(s)
Metals, Heavy/analysis , Spectrophotometry, Atomic/methods , Viper Venoms/chemistry , Animals , Viper Venoms/standards , Viperidae/metabolismABSTRACT
In article comparative literary and experimental data about degree of a level of scrutiny of snake venoms are presented. Obtained data shows that increased pollution of industrial regions results in higher levels of heavy metals in snake venom. Change of physical and chemical parametres, and also pharmacological activity and toxicity of zootoxins under influence biotics, abiotics factors and including heavy metals and radiation is noted.
Subject(s)
Environment , Environmental Exposure/adverse effects , Snake Venoms/chemistry , AnimalsABSTRACT
Irradiation of double-stranded DNA solutions containing 8-methoxypsoralene /8-MOP/ by near ultraviolet light /365 nm/ resulted in the formation of fluorescent photoaddiucts 8-MOP-DNA. The fluorescence of these products in the DNA molecule is considerably quenched due to the intercalation between the bases. After hydrolysis of DNA the quenching was eliminated. The maximal fluorescence of the photoadducts was observed near 460 nm; the maximal excitation was near 328 nm.
Subject(s)
DNA/radiation effects , Methoxsalen/radiation effects , Ultraviolet Rays , Animals , Cattle , Chickens/metabolism , DNA/isolation & purification , Erythrocytes/analysis , Fishes/metabolism , Male , Photochemistry , Species Specificity , Spermatozoa/analysis , Thymus Gland/analysisABSTRACT
Irradiation of the rabbit and human skin by near UV light (wave lengths greater than 320 nm) in the presence of 8-methoxypsoralen produced erythema, pigmentation and some changes in the mechanical and electric properties. All the photobiological effects mentioned were inhibited by alpha-tocopherol if it was applied before irradiation. When applied after exposure to irradiation alpha-tocopherol appeared to be ineffective.
