Evaluation of side effects and compliance to chemotherapy in breast cancer patients at a Nigerian tertiary hospital.

Background: Chemotherapy improves tumour control and survival, but it may be associated with side effects (SEs) which can impair treatment compliance and worsen outcomes. Assessment of patients in routine clinical practice, outside clinical trials, may provide the information on effects of chemotherapy on patients and its impacts on treatment compliance. Aim: To assess the SE and compliance to chemotherapy in breast cancer patients. Methodology: A prospective study involving 120 breast cancer patients receiving chemotherapy was carried out at the oncology clinics of the University College Hospital Ibadan. SEs reported were recorded and graded using Common Toxicity Criteria for Adverse Events version 5. Compliance was defined as a receipt of planned cycles of chemotherapy in the planned doses within the planned duration. The data collected were analysed using the Statistical Package for the Social Sciences software version 25. Results: The patients were all females with a mean age of 51.2 ± 11.8 years. Patients reported between 2 and 13 SE with a median of 8 SE. Forty-two (35.0%) missed at least one course of chemotherapy while 78 (65%) were compliant. The reasons for non-compliance were deranged blood test 17 (14.2%), chemotherapy SE symptoms related 11 (9.1%), financial constraints 10 (8.3%), disease progression 2 (1.7%) and transportation-related 2 (1.7%). Conclusion: Breast cancer patients encounter multiple SEs from chemotherapy which led to non-compliance with the treatment. Early identification and prompt treatment of these SEs will improve compliance with chemotherapy.

Toxicity of insecticidal plant oils on the larval and adult stages of a major malaria vector (Anopheles gambiae Giles 1920).

Despite increasing reports and concerns about the development of resistance to public-health insecticides in malaria vectors, significant progress has been made in the search for alternative strategies to disrupt the disease transmission cycle by targeting insect vectors and thus sustaining vector management. The use of insecticidal plants is a strategy that can be employed and this study investigates the toxicity potential of insecticidal plant oils shortlisted in an ethnobotanical survey on Anopheles gambiae larvae and adult stages. The shortlisted plants parts, the leaves of Hyptis suaveolens, Ocimum gratissimum, Nicotiana tabacum, Ageratum conyzoides, and Citrus sinensis fruit-peel were collected and extracted using a Clevenger apparatus. Larvae and female adults of deltamethrin-susceptible Anopheles gambiae were obtained from an already-established colony at the University of Ilorin's Entomological Research Laboratory. In five replicates, twenty-five third instar stage larvae were used for larvicidal assays and twenty 2-5 days old adults were used for adulticidal assays. After 24 h, An. gambiae exposed to Hy. suaveolens and Ci. sinensis exhibited significantly higher larval toxicity (94.7-100%). The mortality induced by the oils of the four plants peaked at 100% after 48 h. Ni. tabacum (0.50 mg/ml) induced the highest percentage of adult mortality on An. gambiae (100%) when compared to the positive control Deltamethrin (0.05%). The lowest KdT50 was observed with 0.25 mg/ml of Ni. tabacum (20.3 min), and the lowest KdT95 was observed with 0.10 mg/ml of Ag. conyzoides (35.97 min) against adult An. gambiae. The evaluated plant oils demonstrated significant larval and adult mortality rates, lower lethal concentrations, and knockdown times, indicating promising results that can be further developed for malaria vector management.

Lentinus squarrosulus Mont. Mushroom: Molecular Identification, In vitro Anti-Diabetic, Anti-Obesity, and Cytotoxicity Assessment.

Objectives: Mushrooms are fungi with nutritional and health benefits. Lentinus squarrosulus Mont., an edible fungus, has traditional usage and relevance in local therapy for managing metabolic diseases. In that view, this study aimed to evaluate the in vitro anti-obesity, anti-diabetic, and cytotoxic potential of the chloroform/methanol extract (CME) and aqueous extract (AE) of the mushroom. Materials and Methods: L. squarrosulus was identified using molecular biology tools. The CME and AE were obtained sequentially and, then, subjected to α-amylase, α-glucosidase, and lipase inhibitory enzyme assays as well as total phenolic content (TPC) and flavonoid content (TFC) estimations. The cytotoxic potential of extract fractions of L. squarrosulus was assessed using the brine shrimp lethality assay. Results: The molecular identification of the mushroom displayed that the internal transcribed spacer sequence was an equivalent match to that of L. squarrosulus with a high percentage similarity, and thus assigned a unique accession number (KT120043.1). The CME of L. squarrosulus had higher TPC, TFC, and α-glucosidase inhibitory activity than AE. Furthermore, AE of the mushroom showed a higher lipase inhibitory potential with an IC50 value of 22.28 ± 0.65 µg/mL than the CME, while that of the reference, i.e. orlistat was 2.28 ± 0.34 µg/mL. However, these extracts exhibited very low or no α-amylase inhibitory and cytotoxic activity at the tested concentrations. Conclusion: This study reveals that CME of L. squarrosulus, rich in polyphenols and flavonoids, possesses considerable α-glucosidase and lipase inhibitory activities.

Salacia pallescens Oliv. (Celastraceae) Scavenges Free Radicals and Inhibits Pro-inflammatory Mediators in Lipopolysaccharide-activated RAW Cells 264.7 Macrophages.

OBJECTIVES: Salacia pallescens has folkloric anti-inflammatory claims, with little scientific investigation. Hence, the antioxidant and anti-inflammatory effects along with phytochemical components of the plant were investigated. MATERIALS AND METHODS: The antioxidant property of S. pallescens leaf (SPL) methanol extract was evaluated using 1,1-diphenyl-2-picrylhydrazyl and nitric oxide inhibition assays. The anti-inflammatory property of SPL in lipopolysaccharide-stimulated RAW 264.7 macrophages was determined. The cytotoxicity of SPL was assessed in brine shrimp lethality assay (BSL) and against RAW 264.7 cells in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide based assay. Gas chromatography-mass spectrometry was employed to identify SPL phytochemical compounds. RESULTS: SPL significantly scavenged free radical generated in the antioxidant assays and inhibited nitrite production in stimulated RAW 264.7 cells. Similarly, there was a 9-fold reduction in interleukin-6 produced in RAW 264.7 cells when exposed to the highest concentration of SPL. In addition, 50% lethal concentration of SPL was 455.58±82.35 µg/mL while cyclophosphamide gave 16.3±0.15 µg/mL in BSL test. Moreover, cell viability was not affected by SPL. Sixteen compounds were identified from SPL where thymol (29.79%), 3-carene (15.97%), and p-cymene (12.19%) are the most abundant. CONCLUSION: Methanol extract of SPL showed antioxidant and anti-inflammatory activities by free radicals and cytokines inhibition. The activity observed may be related to the polyphenolic compounds in the plant.

Phytochemical Constituents, Antioxidant Activity, and Toxicity Assessment of the Seed of Spondias mombin L. (Anacardiaceae).

OBJECTIVES: Increased generation of free radicals exceeding the antioxidant capacity of the host is deleterious. Thus new, potent, and safe antioxidants will be a valuable addition to the limited antioxidant arsenals available. Therefore, the antioxidant activity, cytotoxicity potential, and phytochemical constituents of the methanol extract of Spondias mombin seed (MESSM) were investigated. MATERIALS AND METHODS: 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and hydrogen peroxide (H2O2) were the antioxidant assays used. The cytotoxicity of MESSM was evaluated against a rhabdomyosarcoma (RD) cell line in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide based assay. The phytochemical constituents of MESSM were identified using gas chromatography-mass spectrometry. RESULTS: MESSM produced better antioxidant activity in the DPPH (IC50=58.64±1.49 µg/mL) and H2O2 (IC50=44.03±5.57 µg/mL) assays than in the NO (IC50=494.55±12.68 µg/mL, p<0.0001) assay. Moreover, MESSM was nontoxic (CC50=139.6±0.54 µg/mL) in comparison to cyclophosphamide (CC50=0.97±0.03 µg/mL) against the RD cell line. The major compounds in MESSM were dodecanoic acid (22.48%), tetradecanoic acid (17.95%), n-hexadecanoic acid (15.35%), capsaicin (12.11%), and dihydrocapsaicin (5.23%). CONCLUSION: The seed extract of Spondias mombin contains nontoxic antioxidant compounds that could be explored in the pharmaceutical and cosmetics industries for the development of antioxidant agents.

Lopinavir/ritonavir enhanced the antimalarial activity of amodiaquine and artesunate in a mouse model of Plasmodium berghei.

Treatment of malaria and HIV in co-infected patients remains a challenge due to the limited information on interaction between drugs used for the treatment of the two infections. Thus, this study evaluated the interaction between lopinavir/ritonavir (LR) and artesunate (AS), amodiaquine (AQ) or a fixed dose of AS/AQ in a mouse model of chloroquine-resistant Plasmodium berghei. Combination of LR with graded doses of AS or AQ resulted in a significant reduced ED50. In addition, parasites cleared completely from day 3 till day 21 post-infection in animals infected, treated with AS/AQ alone or AS/AQ with LR and all the animals survived till day 21 post-infection. In contrast, survival on day 21 in animals treated with AQ alone or AQ with LR was 20 and 60%, respectively. It appears that the protease inhibitor LR enhanced the antimalarial drugs AS and AQ.