ABSTRACT
The ultra and histochemical structures of the oncosphere of Hymenolepis nana were studied. The oncosphere is surrounded by several protective layers. The outer envelope appears to be cellular in origin and consist of an electron dense outer zone and an inner granular zone, mucoprotein in nature. The inner envelope and embryophore are a continuous morphological unit of a protein structure. Within the oncosphere, apart from hooks, there are two germ cells, two somatic cells and a large penetration gland. The oncospheral hooks consist of three layers and elicit a positive reaction for keratin. The penetration glands fill the posterior region of the embryo and contain granules of varying density and shape.
Subject(s)
Hymenolepis/ultrastructure , Animals , Histocytochemistry , Microscopy, ElectronABSTRACT
Only on the basis of nuclear size, spermatogonia can be easily identified by their relatively large prominent nuclei. Further meiotic division occur resulting in primary spermatocytes. The nuclei become smaller, placed peripherally forming the spermatids. A membrane-bound clefts develop in the cytoplasm in the region of spermatid nuclei and that such clefts eventually enclose the elongated nuclei to become the plasma membrane of the sperm. There is a common general pattern of spermatogenesis in cestodes which follows the pattern typical of the platyhelminthes.
Subject(s)
Hymenolepis/physiology , Spermatogenesis/physiology , Spermatozoa/ultrastructure , Animals , Cell Nucleus/ultrastructure , Male , Microscopy, ElectronABSTRACT
Scanning electron microscopy of the surface of Hymenolepis nana indicated that dense populations of microtriches occur on scolex proper, suckers and strobila, with an average density of 20/micron2. The excellent preservation of microtriches proves the efficacy of the critical point drying method for preparing cestodes for study of SEM. The cytological structure of the tegument of H. nana corresponds in general to that of other tapeworms.
Subject(s)
Hymenolepiasis/parasitology , Hymenolepis/ultrastructure , Animals , Male , Mice , Microscopy, Electron , Microscopy, Electron, ScanningABSTRACT
The sensitivity of the technique of immunoelectrodiffusion has been enhanced by combining it with an enzyme method. The precipitated immuno-complexes are treated with antibodies conjugated with an enzyme specific for each class of immuno-globulins. This association realizes the ELIEDA and enable the class of immunoglobulins involved in the reactions to be determined. The technique allows the follow up and the qualitative evaluation of the bilharzial antibody in natural and experimental affections.
