ABSTRACT
We did a prospective study of two groups of patients having elective cholecystectomy through a right subcostal incision to evaluate the effectiveness of transcutaneous electrical nerve stimulation (TENS). Of 64 patients, 30 received TENS postoperatively and 34 did not. Narcotic use in total dose or on a weight basis was not statistically different between the two groups, nor was there any difference in the occurrence of pulmonary or gastrointestinal complications. The use of TENS after this particular operation did not appear to lessen narcotic use or pain-related complications.
Subject(s)
Cholecystectomy/adverse effects , Electric Stimulation Therapy , Pain, Postoperative/therapy , Transcutaneous Electric Nerve Stimulation , Evaluation Studies as Topic , Female , Humans , Male , Middle Aged , Narcotics/therapeutic use , Pain, Postoperative/etiology , Prospective Studies , Random AllocationABSTRACT
Four monolayer mammalian cell lines were cured of Mycoplasma hyorhinis infections by cloning in microtiter dishes in the presence of tetracycline and kanamycin. During cloning, cultures were refed with fresh antibiotic containing medium every 2 or 3 d for 14 day and were then cultured without effective antibiotics for at least 21 d. From the four lines we recovered 29 clones, none of which were infected after treatment as judged by the lack of extranuclear fluorescence after staining with the fluorochrome Hoechst 33258, and by normal autoradiographic labeling of the cells by tritiated nucleosides. One clone from each line was tested further by attempted culture of mycoplasmas and was also judged to be uninfected. Infection has not reappeared in any of the clones after extensive culture in the absence of the effective antibiotics.
