ABSTRACT
Fatigue is a common and disabling symptom of multiple sclerosis (MS), a classical Th1- and Th17-mediated autoimmune disease. There is no effective pharmacological treatment for fatigue, but some reports point towards beneficial effects of physical activity on management of the fatigue in MS patients. As both MS and fatigue have been associated with dysregulated cytokine network production, the objective of the present study was to evaluate the impact of a physical activity program consisting of a 12-week series of combining Pilates and aerobic exercises on fatigue severity, determined by FSS, and cytokine production, quantified by ELISA, by T cells from MS patients (n=08) with low disability (EDSS≤2). The results showed decrease in FSSs in all patients at the end of physical activity intervention. Regarding the cytokines, a significant reduction of IL-22 release was observed in polyclonally-activated T cells form MS patients post-training follow-up. Interestingly, while the physical activity attenuated the ability of dopamine in up-regulating Th17-related cytokines, it enhanced the anti-inflammatory effects of serotonin, evidenced by high IL-10 production. In summary, all results suggest that programmed physical activity has beneficial effects on management of fatigue in MS patients, and it could be related, at least in part, to its ability in regulating neuroimmune parameters into T cell compartment.
Subject(s)
Cytokines/metabolism , Exercise Therapy/methods , Fatigue/etiology , Multiple Sclerosis , T-Lymphocytes/metabolism , Adult , Disability Evaluation , Dopamine/pharmacology , Exercise , Exercise Movement Techniques/methods , Female , Humans , Male , Middle Aged , Multiple Sclerosis/complications , Multiple Sclerosis/pathology , Multiple Sclerosis/rehabilitation , Serotonin/pharmacology , Statistics as Topic , T-Lymphocytes/drug effectsABSTRACT
Examinou-se a açäo antibacteriana de extratos vegetais a partir de 120 espécies de plantas pertencentes a 28 famílias: Anacardiaceae (1), Araliaceae (1), Boraginaceae (1), Buddlejaceae (1), Burseraceae (1), Compositae (79), Convolvulaceae (1), Crassulaceae (1), Cruciferae (1), Cyperaceae (1), Eriocaulaceae (1), Flacourtiaceae (1), Gramineae (1), Labiatae (3), Lauraceae (3), Leguminosae (4), Liliaceae (1), Magnoliaceae (1), Moraceae (1), Myrtaceae (3), Polygalaceae (1), Rutaceae (3), Saxifragaceae (1), Solanaceae (2), Theaceae (2), Verbenaceae(3), Vochysiaceae (1) e Zingiberaceae (1). Foram confeccionados e avaliados 301 extratos obtidos destes vegetais inteiros ou de parte deles com solventes orgânicos de diferentes polaridades: hexano, hexano-acetato de etila 20//, acetado de etila, etanol e metanol. A avaliaçäo antimicrobiana foi realizada através de adaptaçäo do método de Kirby-Bauer usando discos de papel de filtro contendo soluçöes dos extratos e que foram depositados sobre culturas de Pseudomonas aeruginosa e Staphylococcus aureus, e 81 extratos, preparados a partir de 58 plantas, foram ativos contra S. aureus e 5 extratos, obtidos de 4 plantas, inibiram o desenvolvimento de P. aeruginosa
