Subject(s)
Pituitary Diseases/immunology , Rhinitis, Allergic, Seasonal/immunology , Allergens , Dust , Humans , Immunoglobulins/analysis , Immunologic Deficiency Syndromes/complications , Mucus/immunology , Nasal Mucosa/metabolism , Pituitary Diseases/etiology , Respiratory Hypersensitivity/complications , Rhinitis/immunology , Silicon DioxideABSTRACT
By counterimmunoelectrophoresis in agarose gel the presence of alpha 2-PGA in the sera of 28 untreated patients with different localization of cancer was demonstrated. In 17 (60.7%) a precipitation line between the well with patient serum and that with monospecific anti-alpha 2-PGA serum, diluted 1 : 7 was obtained. At 1 : 7 dilution, with the sera from 28 healthy persons precipitation lines were not obtained. The determination of alpha 2-PAG can be made quickly and contributes to cancer diagnosis in men.
Subject(s)
Neoplasms/blood , Pregnancy Proteins/analysis , Pregnancy-Associated Plasma Protein-A/analysis , Aged , Counterimmunoelectrophoresis , Humans , Male , Middle AgedSubject(s)
Antibodies , Aqueous Humor/immunology , Uveitis/immunology , Antibodies/analysis , Humans , Immunoelectrophoresis , Uveitis/diagnosisABSTRACT
Rheumatoid factors were present in 33.2%, crioglobulins in 47.6%, and anticomplementary assay in 34.2% of cases with chronic active hepatitis. No statistically significant loss of total complement was observed, but its components especially C1 and C3, exhibited lowered titers in 48.6% and respectively 45.7% of 160 cases. The origin, significance and pathogenicity of these factors are discussed in connection with the presence and possible role of the immunologic complexes to the development of chronic liver injury.
Subject(s)
Antigen-Antibody Complex , Hepatitis/immunology , Chronic Disease , Complement Inactivator Proteins , Complement System Proteins/analysis , Cryoglobulins/analysis , Humans , Rheumatoid Factor/analysisABSTRACT
A method is described for increasing the sensitivity of conventional counterimmunoelectrophoresis (CIEP), i.e. double sequential counterimmunoelectrophoresis (DCIEP). Our method consists of a second electrophoretic migration made after the classical CIEP, where instead of HB antibody (from polytransfused patients), the anodic wells are filled with an antihuman-gammaglobulin antiserum. DCIEP is significantly more sensitive than CIEP (P less than 0.01), the number of positive sera in chronic lever diseases being 28.7% compared with 17.5%.
Subject(s)
Hepatitis B Antigens/analysis , Chronic Disease , Counterimmunoelectrophoresis/methods , Humans , Liver Diseases/diagnosis , Liver Diseases/immunology , MicrochemistryABSTRACT
Double sequential counterimmunoelectrophoresis (DCIEP) (an improved method that we have developed) and Ausria II-125I (an indirect solid-phase radioimmunoassay) were compared for HBAg detection in chronic liver diseases. 26.8% of the patients were positive using DCIEP and 41.8% were positive with Ausria II125I. The conventional counterimmunoelectrophoresis (CIEP) is less sensitive than DCIEP, the level of positive results being 18.8% (P less than 0.001)9
