Ozone treatment of recurrent aphthous stomatitis: a double blinded study.

This study aimed to evaluate the use of ozone to treat recurrent aphthous stomatitis (RAS). Consecutive sixty-nine participants with RAS were recruited into this non-randomized double blind, controlled cohort observational study (test group). A control group of 69 RAS patients who matched test group with age and gender was recruited. RAS lesions in test group were exposed to ozone in air for 60 seconds while controls received only air. Ulcer size and pain were recorded for each participant at baseline and daily for 15 days. Ulcer duration was determined by recording the time taken for ulcers to disappear. The main outcome measures were pain due to the ulcer, ulcer size and ulcer duration. 138 RAS participants (69 participants and 69 controls) were analyzed. Ulcer size was reduced starting from the second day in test group and from the fourth day in controls (p ≤ 0.004). Pain levels were reduced starting from the first day in the test group and from the third day in controls (p ≤ 0.001). Ulcer duration, ulcer size after day 2 and pain levels were more reduced in the test group. In conclusion, application of ozone on RAS lesions for 60 seconds reduced pain levels and enhanced ulcers' healing by reducing ulcers' size and duration.

Improved tooth bleaching combining ozone and hydrogen peroxide--A blinded study.

OBJECTIVES: To evaluate the efficacy of tooth bleaching using ozone after hydrogen peroxide (H2O2) in comparison to the use of H2O2 alone. METHODS: 70 extracted teeth were randomly distributed into two groups. Teeth surfaces in group 1 (n=35) were treated using 38% H2O2 and then were exposed to ozone for 60s and this ozonated peroxide mixture was left on the teeth for 20 min. Meanwhile, teeth in group 2 (n=35) were treated with H2O2 38% for 20 min. The L* a* b* and Vita Classic shade values of teeth were evaluated in both groups at base line, after application of H2O2 and ozone in group 1, and after application of H2O2 and then again after another application of ozone in group 2. The statistically significant changes were set at P ≤ 0.05. RESULTS: Baseline L* a* b* and Vita shade values were comparable between groups (P>0.05). Teeth obtained lighter shades following bleaching with both H2O2 and ozone or with H2O2 alone (P ≤ 0.05). Further bleaching with ozone for teeth already bleached with H2O2 alone showed further improvement of the shades of teeth (P<0.001). Teeth treated with H2O2 and ozone had more shade improvements than those only treated with H2O2 (P<0.001). Also, L* values were increased while b* values were decreased (teeth obtained lighter shades) following bleaching in both groups (P ≤ 0.05). More changes were obtained when both ozone and H2O2 were used (P ≤ 0.05). CONCLUSIONS: Bleaching with 38% H2O2 and ozone resulted in teeth with lighter shades than bleaching with 38% H2O2 alone.

Comparison of dental bleaching effects of ozone and hydrogen peroxide: An ex vivo study.

PURPOSE: To compare the efficacy of using ozone versus hydrogen peroxide (H2O2) for tooth bleaching. METHODS: 90 extracted teeth were allocated into two groups. Teeth in Group 1 (n= 45) were exposed to ozone for 60 seconds; ozone was produced by healOzone x4 machine (healOzone x4), and was applied to teeth through special disposable silicone cups. The silicone cups allowed total seal and avoided gas escape as the machine is devised to work only if the cup guarantees perfect seal and thus secure the safety of the machine for human use. Teeth in Group 2 (n= 45) were treated with 38% H2O2 for 20 minutes. The shade of teeth was recorded for both groups at base line, after application of ozone in Group 1, and after application of H2O2 in Group 2. The reading for L* a* b* values and Vita Classic shades were recorded using the Colorimeter Konica-Minolta CR-400. The statistically significant differences were set at P≤ 0.05. RESULTS: The teeth in both groups acquired lighter shades after bleaching (P< 0.001). In addition, baseline L* shade values were increased and b* shade values were decreased (means lighter shades) after bleaching in both groups (P< 0.01). However, baseline a* shade values were not significantly changed after bleaching with ozone in Group 1 (P= 0.682). In contrast, a* shade values were significantly decreased after bleaching with H2O2 in Group 2 (P= 0.005). Furthermore, final shades achieved after bleaching were not significantly different between groups (P> 0.05). In conclusion, application of ozone for 60 seconds or application of 38% of H2O2 for 20 minutes resulted in teeth with lighter shades. Bleaching by application of ozone for 60 seconds would result in similar outcomes to bleaching with 38% H2O2. CLINICAL SIGNIFICANCE: This is the first study to compare bleaching effects of ozone and hydrogen peroxide on natural human teeth. It might be suitable to consider ozone for dental bleaching since comparing to H2O2 it could potentially reduce the time, side effects and cost of treatment. Also, application of ozone is better controlled and more convenient and measurable as it is delivered by a machine that can manage the concentration, volume, delivery site and timing of delivered ozone.