ABSTRACT
We report here studies on the antistress protective actions of three peptides of the glyproline family: Pro-Gly-Pro, Pro-Gly, and Gly-Pro. Stress (10 min forced swimming) evoked typical changes in the behavioral activity of rats in the elevated cross maze and hole board tests, providing evidence of a significant increase in anxiety and a decrease in the level of orientational-investigative activity. Prior (15 min before stress) i.p. administration of Pro-Gly-Pro and Gly-Pro at a dose of 3.7 microM/kg significantly decreased the stress-induced behavioral abnormalities. This demonstrates the possibility that peptides Pro-Gly-Pro and Gly-Pro may affect CNS structures involved in forming the body's responses to stress-inducing factors. Peptide Pro-Gly, at an equimolar dose, had no marked protective effect and only slightly decreased the stress-induced abnormalities in the behavior of rats.
Subject(s)
Behavior, Animal/drug effects , Behavioral Symptoms/drug therapy , Behavioral Symptoms/etiology , Dipeptides/therapeutic use , Neuroprotective Agents/therapeutic use , Stress, Physiological/complications , Animals , Dipeptides/chemistry , Exploratory Behavior/drug effects , Male , Maze Learning/drug effects , RatsABSTRACT
Protective and antistress effects of three glyprolines--PGP, PG and GP, were studied. Stress influences produced typical changes of behavioural activity of rats in the elevated pluz-maze and the hole-board tests. These changes suggest a significant enhancement of anxiety and a drop of the level of orientation-investigative activity. A preliminary (15 minutes before stress agent) intraperitoneal administration of PGP or GP in doses of 3.7 microm/kg significantly decreased stress disturbances of behaviour. Analysis of these data shows to the possibility of PGP and GP influences on the CNS structures, which take part in the organism reciprocal reactions to stress factor. The peptide GP at equimolar dose didn't possess pronounced protective properties and just slightly decreased stress disturbance of behavioural activity of rats.
Subject(s)
Behavior, Animal/drug effects , Dipeptides/administration & dosage , Maze Learning/drug effects , Stress, Physiological/physiopathology , Animals , Injections, Intraperitoneal , Male , RatsABSTRACT
On the experimental model of denervation-reinnervation syndrome of mouse shank muscles neurotrophic effects of jashaltinsky medicinal mud were studied. In experimental mice therapeutic mud has been applied on the small back, tail and rib areas of the body 10 days after nerve crushing. On day 11 after nerve crushing a stable reproduction of a compound nerve action potential with constant amplitude was revealed in repetitive (50 Hz) nerve stimulation.
Subject(s)
Mud Therapy , Muscle Denervation , Muscle, Skeletal/innervation , Nerve Regeneration/drug effects , Peroneal Nerve/physiology , Peroneal Neuropathies/therapy , Animals , Disease Models, Animal , Male , Mice , Muscle, Skeletal/drug effects , Peroneal Nerve/drug effects , SyndromeABSTRACT
The Republic of Kalmykia possesses a great deal of therapeutical peloids awaiting biomedical investigations. Mud formation in Kalmykia is characterized by an intensive accumulation of sulfide salt. Therapeutical peloids form in the Manych Lake region. An experimental murine model of acetate-induced gastric ulcer was used to evaluate the therapeutical efficacy of Manych mud. There was a statistically significant difference in the rate of ulcer healing between the study and control groups.
Subject(s)
Mud Therapy , Peptic Ulcer/therapy , Acetic Acid , Animals , Male , Peptic Ulcer/chemically induced , Peptic Ulcer/pathology , RatsABSTRACT
The infusion of a small volume of blood with temperature higher ("warm") or below ("cold") the temperature of atrial blood into the right atrium induced "warm" tachycardia and "cold" bradycardia, resp., in anesthetized and in alert cats. Infusion of the blood with temperature equal to that of atrial blood induced no changes of the heart rate. Infusion of the same volume of "cold" or "warm" blood after adreno- and cholinoreceptor-blocking agents was accompanied by a less severe tachycardia or bradycardia. There seem to be two mechanisms in the initiation of the above effects: the neural and the myogenic ones.
Subject(s)
Bradycardia/physiopathology , Cold Temperature , Hot Temperature , Tachycardia/physiopathology , Adrenergic alpha-Antagonists/pharmacology , Adrenergic beta-Antagonists/pharmacology , Animals , Blood Physiological Phenomena , Body Temperature , Bradycardia/etiology , Cats , Heart Rate/drug effects , Receptors, Adrenergic/drug effects , Receptors, Adrenergic/physiology , Receptors, Cholinergic/drug effects , Receptors, Cholinergic/physiology , Tachycardia/etiology , Vagus Nerve/physiology , VeinsABSTRACT
A review of literary and own experimental data is given. More adequate method in the study of the hypothalamic heart regulation is the investigation of the hypothalamic influences on the cardiac reflexes. The necessity of the experiments on the unanesthetized unrestrained animals is underlined. The dual modulating hypothalamic influences existing already in fishes having been revealed on the unanesthetized animals. The character of the modulating influence is determinated to a certain extent by the cardiac reflex intensity: small responses are increased, larger ones are reduced. The extension of system regions involved into the reflex response pattern at the increase of the degree of temperature effect of venous blood on heart has been discovered in unanesthetized unrestrained cats.
Subject(s)
Heart/physiology , Hypothalamus/physiology , Reflex/physiology , Animals , Autonomic Nervous System/physiology , Blood Physiological Phenomena , Body Temperature , Cardiovascular Physiological Phenomena , Cats , Electric Stimulation , Fishes , Hemodynamics , Neural Inhibition , Reflex, Abnormal/physiopathology , Veins , Wakefulness/physiologyABSTRACT
The heart rate changes in response to the imitation of venous input to the heart by infusion of an additional blood volume to the right atria have been studied on anesthetized by chloralose cats. It has been discovered that patterns of the heart rate changes are determined by the infused blood temperature if it is equal to the blood temperature in heart, tachycardia appears, if it is below this temperature, bradycardia develops. These data explain, from our point of view, the contradictory results obtaining by different authors in experiments with the imitation of the increased venous input (Bainbridge reflex).
