ABSTRACT
Se investigo si la inmunizacion con celulas del linfoma murino L5178Y inactivadas por radiacion induciria una reaccion inmunitaria de rechazo hacia las celulas intactas de ese linfoma. La dosis de radiacion necesaria para inactivar las celulas del linfoma fue de 6,000 rads, suministradas por un emisor de 60 Co. Se inmunizaron 26 ratones BALB/C con cuatro inyecciones por via intraperitoneal una cada ocho dias de 5x10 (6) celulas L5178Y radiadas. Diez dias despues se les aplico por la misma via una dosis de prueba de 5x10 (7) celulas L5178Y intactas. Los testigos fueron otros 20 ratones no inmunizados que recibieron el mismo inoculo de celulas L5178Y. Los ratones se sacrificaron ocho dias despues, y se evaluo el crecimiento por el numero de celulas L5178Y producidas. Los ratones testigos desarrollaron un promedio de 10.05+/-0,02 x 10 (8) celulas tumorales. Los ratones inmunizados con las mismas celulas radiadas desarrollaron 25.20+/-0.03 x 10 (8)celulas L5178Y (p <0.001). Estos resultados mostraron que las celulas del linfoma murino L5178Y inactivadas por radiacion no indujeron una reaccion de rechazo hacia las mismas celulas viables, sino que la inmunizacion con ellas causo un incremento del desarrollo tumoral en los ratones inmunizados
Subject(s)
Animals , Mice , Immunization , Leukemia L5178ABSTRACT
It has been reported that a great part of the deleterious effect caused by ionizing radiations is due to oxidative phenomena. The purpose of this work was to investigate if some antioxidants commonly used in the food industry might have a radioprotecting effect. N propyl-galate (NPG), hydroxyanisol-butylated (HAB) and hydroxytoluene-butylated (HTB) were utilized. Forty BALB/C mice that received 600 rads of gamma irradiation from a 60Co emitter died 17.12 +/- 7.82 days after. Thirteen mice that were injected 5 mg of NPG 24 hours and 30 minutes before radiation survived for more than 90 days. This same effect was observed in 13 mice that were also injected with 10 mg NPC and in other 13 that received 10 mg of HAB in the same way. Doses of 5 mg HAB or 5 and 10 mg HTB did not have a radioprotecting effect. When the radiation dose was increased to 800 rads, the radioprotecting effect was absent with any of the NPG, HAB or HTB doses. In protected mice, preservation of higher numbers of hematopoietic cells were observed in the bone marrow together with slight reduction of chromosomal fractures. There results show that antioxidants used in the food industry have a radioprotecting effect that had not been investigated.
Subject(s)
Anisoles/pharmacology , Antioxidants/pharmacology , Butylated Hydroxyanisole/pharmacology , Food Preservatives/pharmacology , Gallic Acid/analogs & derivatives , Propyl Gallate/pharmacology , Radiation-Protective Agents/pharmacology , Animals , Bone Marrow/radiation effects , Butylated Hydroxytoluene/pharmacology , Chromosomes/radiation effects , Gamma Rays , Mice , Mice, Inbred BALB C , Radiation Injuries, Experimental/prevention & controlABSTRACT
Radiosensitivity of L5178/ murine lymphoma was studied in vivo by means of Co60 pump radiations. The dosage necessary to inactivate these cells was from 5,000 to 6,000 rads applied in an only dose. Doses under 2,000 to 4,000 rads only achieved partial inactivation, proportional to dosage applied. 100 to 1600 rad doses did not produce adverse effects on these cells. Lethal dose for carrier mice was from 800 to 1200 rads applied in one dose only or a total of over 600 rads applied in 100 rad daily sessions. High radioresistance of lymphoma did not allow destruction of all neoplastic cells in vivo for, before this occurred carrier mice died.
