ABSTRACT
The hypothesis that failure of histamine (Hi)-mediated inter and intracellular cell-cell communication may be involved in the control of cellular growth has been tested in leukaemia-bearing mice and fibrosarcoma-bearing rats. In all examined tissues of mice bearing leukaemia L1210 cells and rats bearing methylcholanthrene fibrosarcoma histamine content was higher than in controls. Tissues of fibrosarcoma-bearing rats more intensively metabolized 14C-Hi and oxidative pathway was predominant. Histamine metabolic activity as well as histamine catabolic pathways do not differ in leukaemic mice as compared with the controls.
Subject(s)
Histamine/metabolism , Neoplasms, Experimental/metabolism , Animals , Fibrosarcoma/metabolism , Histamine/analysis , Leukemia L1210/metabolism , Mice , Mice, Inbred Strains , Rats , Rats, Inbred StrainsABSTRACT
The effect of an industrial agent--Fasavin E4, on isolated rat mast cells has been investigated. It was shown that Fasavin E4 released histamine in concentration dependent fashion. The reaction was neither calcium nor energy dependent. It is concluded that histamine release induced by Fasavin E4 does not conform to secretion and possibly occurred due to membrane damage by cytolytic process.
Subject(s)
Histamine Release/drug effects , Polyethylene Glycols/pharmacology , Animals , Calcium/physiology , In Vitro Techniques , Mast Cells/metabolism , Rats , Rats, Inbred Strains , Textile IndustryABSTRACT
The inflammatory effect of some compounds (polyglycolamines and polyglycolalcohols condensated with various number of ethylene oxide groups) used in textile industry were investigated as to their action on skin vascular permeability in rats and histamine release from rat peritoneal mast cells. The intradermal injection of tested agents caused the inflammatory skin reactions of various intensity depending on time, dose and chemical structure of agent used. It has been also shown that these properties of tested compounds were correlated with their histamine releasing activity and seemed to be dependent on their chemical structure, namely, the number of ethylene oxide groups in molecule. It is suggested that the histamine release induced by tested compounds is of cytotoxic nature since it depends neither on metabolic energy nor on extracellular calcium.
Subject(s)
Histamine Release/drug effects , Polyethylene Glycols/toxicity , Textile Industry , Amino Alcohols/toxicity , Animals , Capillary Permeability , Female , Inflammation/chemically induced , Male , Mast Cells/drug effects , Rats , Skin/drug effectsABSTRACT
The action of various salts of acetylcholine on isolated mast cells from Wistar and F1 hybrids of Wistar and August rats was investigated. None of the acetylcholine salts within the concentration range 10(-12) M to 10(-2) M was able to release histamine either from Wistar or from hybrid mast cells. Compound 48/80, used as a control, was active in both cases. The results obtained are in opposition to some recent reports. The possible reasons for these contradictions are discussed.
Subject(s)
Acetylcholine/pharmacology , Histamine Release/drug effects , Mast Cells/drug effects , Animals , Bromides/pharmacology , Chlorides/pharmacology , Female , Iodides/pharmacology , Male , Mast Cells/metabolism , RatsABSTRACT
The effect of various fluorides as to their ability to activate mast cells to subsequent secretory action of calcium have been investigated. The mechanism of action of both potassium and lithium fluoride conformed to a secretory process by being dependent on calcium and cellular metabolic energy. On the other hand, the action of ammonium fluoride seemed to be cytotoxic. Among fluorides tested, potassium fluoride was shown to be the most potent agent. The potency of mast cell activatory action of fluorides was parallel to their dissociation constants.
Subject(s)
Fluorides/pharmacology , Mast Cells/drug effects , Animals , Calcium/pharmacology , Female , Histamine Release/drug effects , Male , Mast Cells/metabolism , RatsSubject(s)
Histamine Release/drug effects , Lidocaine/pharmacology , Zinc/pharmacology , Animals , Female , Hemocyanins , Kinetics , RatsABSTRACT
The effect of complexes of lidocaine with zinc, copper and cobalt on histamine release from peritoneal rat mast cells induced by various secretagogues was investigated. The complexes of lidocaine with metal ions inhibited histamine release induced by compound 48/80 and ionophoreous antibiotics: A23187 and X537A. The ionic complex of lidocaine with zinc was found to be the most potent against 48/80--induced histamine release and exerted the significant inhibition in the concentration of 10(-5) M. The action of coordination complex of lidocaine with zinc was somewhat weaker. The significant inhibitory effect on 48/80-induced histamine release of the copper and cobalt lidocaine complexes was observed in concentration of 10(-4) M. Histamine release by ionophores A23187 and X537A was also affected by zinc lidocaine complexes. However, coordination complex had more potent action on histamine release than the ionic one.
