Is it possible to optimise the labour and time intensity of diatom analyses for determination of the Polish Diatom Indices (IO, IOJ)?

Diatom-based indices are a recommended approach in the assessment of the ecological status of lakes and rivers, required by the EU Water Framework Directive. To optimise the time-consuming microscopic analyses, we determined the lowest number of diatom valves needed to be identified ('counted') in a sample to arrive at a reliable ecological status assessment based on the Polish multimetric diatom indices (diatom index, IO for rivers, and diatom index for lakes, IOJ). We compared the indices calculated with different valve counts. The dataset consisted of diatom counts in 55 samples collected in the rivers Ina and Drawa (Western Pomerania, NW, Poland) and their lakes. Following the method used by the Polish Chief Inspectorate for Environmental Protection (ChIEP), periphyton samples were collected from macrophytes growing in the rivers and lakes, and diatom slides were prepared. Originally, the ecological status was determined based on identifying 400 valves per slide. In the subsequent simulation, 50, 100, 150, 200, 250, 300, 350 and 400 valves per slide were randomly drawn 20 times for each sample and each simulation, and the indices were calculated again. In 87% of our simulations, the ecological status turned out to be identical, regardless of the number of valves identified. However, the standard deviation of the index values was at its highest (> 0.05) with 50 valves, and decreased systematically (below 0.05) for 100 and more valves. Simulated datasets showed logarithmic trend of changes where the compilation and standard deviation was stable above 200 valves identified. Therefore, we recommend simplified methodology for Polish multimetric diatom indices. Reduction of identified valve quantity in a sample to 200 valves would result in over 95% of compliance with classification obtained when 400 valves were identified.

The Use of a Solid Bismuth Microelectrode for Vanadium Quantification by Adsorptive Stripping Voltammetry in Environmental Water Samples.

This paper presents for the first time the use of an environmentally friendly solid bismuth microelectrode for the voltammetric quantification of V(V) in natural water samples. These studies were designed to replace the film bismuth electrode that had been introduced to eliminate the conventional sensors based on highly toxic mercury. In the proposed procedure, V(V) is preconcentrated at the solid bismuth microelectrode surface via the formation of electroactive complexes with cupferron from a solution of 0.1-mol L-1 acetate buffer, pH = 4.6 at a potential of -0.4 V. The linearity of the calibration graph is in the V(V) concentration range from 8 × 10-10 to 1 × 10-7 mol L-1 with a preconcentration time of 1 min. The limit of detection (calculated as 3 σ) is 2.5 × 10-10 mol L-1 for a preconcentration time of 1 min. It was also demonstrated that significant improvement in analytical parameters was achieved as a result of the activation of the solid electrode surface at a potential of -2.5 V for 2 s. The developed procedure is highly selective for the presence of foreign ions and organic compounds in tested samples. The accuracy of the recommended procedure was checked using SPS-WW1 waste water-certified reference materials of a complex composition, in which the concentration of V(V) determined by the proposed method was 95.1 ± 1.6 ng mL-1. Moreover, in keeping with the outlined procedure, river, tap and rain water samples were analyzed without any pretreatment, and recovery values from 96% to 106% were obtained.

Is There a Link between Balance and Body Mass Composition in Children and Adolescents?

School-age children are particularly prone to disturbances in body composition, because this is a period of intensive growth and a period in which correct habits are shaped, especially in relation to diet. This is why it is so important to diagnose emerging disorders early so as to implement therapeutic or educational activities. The aim of this study was to assess the relationship between the factors of body mass composition and body mass index (BMI), as well as the balance parameters in children and adolescents. The study group consisted of 1137 children aged 7 to 15. There were 559 girls and 578 boys among the subjects. The study used the Tanita 780 body mass composition analyser and the Zebris stabilometric platform. It was found that girls were characterized by a significantly higher content of adipose tissue (24.37% vs. 20.45%), while boys were characterized by a higher content of lean tissue (32.99% vs. 30.43%), muscle tissue (31.23% vs. 28.86%) and water (24.15% vs. 22.28%). Interestingly, the girls had better balance than their peers in all analyzed parameters (COF TTL.-616.72 vs. 661.50; CEArea-73.63 vs. 112.24; COF HD-3.44 vs. 4.23; COF VD-4.52 vs. 5.12). It turned out that among children in adolescence, a higher adipose tissue content and a higher BMI correlated with a smaller surface area (p < 0.05) defined by the center of gravity and smaller deviations of the center of gravity in the horizontal plane (p < 0.05). Sex and adolescence play an important role in differentiating both body composition and body balance. The results of this study allow us to conclude that children with higher BMI values have better balance. Due to the fact that these conclusions are inconsistent with those of other researchers, it will be worth continuing the research (e.g., on a different population group) in order to confirm the results and to draw far-reaching conclusions.

New Strategies for the Simple and Sensitive Voltammetric Direct Quantification of Se(IV) in Environmental Waters Employing Bismuth Film Modified Glassy Carbon Electrode and Amberlite Resin.

An analytical procedure regarding the determination of selenium(IV) by anodic stripping voltammetry exploiting the in situ plated bismuth film electrode is described. Since organics are commonly present in untreated natural water samples, the use of Amberlite XAD-7 resin turns out to be quite important to avoid problems such as the adsorption of these compounds on the working electrode. The optimum circumstances for the detection of selenium in water using differential pulse voltammetry techniques were found to be as follows: 0.1 mol L-1 acetic acid, 1.9 × 10-5 mol L-1 Bi(III), 0.1 g Amberlite XAD-7 resin, and successive potentials of -1.6 V for 5 s and -0.4 V for 60 s, during which the in situ formation of the bismuth film on glassy carbon and the accumulation of selenium took place. The current of the anodic peak varies linearly with the selenium concentration ranging from 3 × 10-9 mol L-1 to 3 × 10-6 mol L-1 (r = 0.9995), with a detection limit of 8 × 10-10 mol L-1. The proposed procedure was used for Se(IV) determination in certified reference materials and natural water samples, and acceptable results and recoveries were obtained.

Changes in Children's Body Composition and Posture during Puberty Growth.

The main goal of our study was to determine how the age of children, puberty and anthropometric parameters affect the formation of body composition and faulty body posture development in children. The secondary goal was to determine in which body segments abnormalities most often occur and how gender differentiates the occurrence of adverse changes in children's body posture and body composition during puberty. The study group consisted of 464 schoolchildren aged from 6-16. Body posture was assessed with the Zebris system. The composition of the body mass was tested with Tanita MC 780 MA body mass analyzer and the body height was measured using a portable stadiometer PORTSTAND 210. The participants were further divided due to the age of puberty. Tanner division was adopted. The cut-off age for girls is ≥10 years and for boys it is ≥12 years. The analyses applied descriptive statistics, the Pearson correlation, stepwise regression analysis and the t-test. The accepted level of significance was p < 0.05. The pelvic obliquity was lower in older children (beta = -0.15). We also see that age played a significant role in the difference in the height of the right pelvis (beta = -0.28), and the difference in the height of the right shoulder (beta = 0.23). Regression analysis showed that the content of adipose tissue (FAT%) increased with body mass index (BMI) and decreased with increasing weight, age, and height. Moreover, the FAT% was lower in boys than in girls (beta negative equal to -0.39). It turned out that older children (puberty), had greater asymmetry in the right shoulder blade (p < 0.001) and right shoulder (p = 0.003). On the other hand, younger children (who were still before puberty) had greater anomalies in the left trunk inclination (p = 0.048) as well as in the pelvic obliquity (p = 0.008). Girls in puberty were characterized by greater asymmetry on the right side, including the shoulders (p = 0.001), the scapula (p = 0.001) and the pelvis (p < 0.001). In boys, the problem related only to the asymmetry of the shoulder blades (p < 0.001). Girls were characterized by a greater increase in adipose tissue and boys by muscle tissue. Significant differences also appeared in the body posture of the examined children. Greater asymmetry within scapulas and shoulders were seen in children during puberty. Therefore, a growing child should be closely monitored to protect them from the adverse consequences of poor posture or excessive accumulation of adipose tissue in the body.

Occupational burnout among active physiotherapists working in clinical hospitals during the COVID-19 pandemic in south-eastern Poland.

BACKGROUND: Occupational burnout, which is more and more commonly encountered among medical professionals and investigated by researchers worldwide, may in particular affect health care workers during the COVID-19 pandemic. OBJECTIVES: The aim of the study was to assess the risk of occupational burnout among physiotherapists working actively in clinical hospitals in south-eastern Poland during the COVID-19 pandemic. METHODS: The level of burnout among the studied physiotherapists was assessed using the Polish version of the Maslach Burnout Inventory by Maslach (MBI). The study was conducted from 20 March to 3 May, 2020 among physiotherapists working professionally during the COVID-19 pandemic in the south east of Poland, during which time health services related to therapeutic rehabilitation were suspended. The study was conducted among 1,540 physiotherapists with a license to practice who worked in clinical departments. Considering the inclusion and exclusion criteria, 106 physiotherapists were qualified for the study. RESULTS: The current findings show that during the COVID-19 pandemic physiotherapists present high burnout rates in all three dimensions: EE (Mean 32.31; CI 29.47-35.15); DP (Mean 16.25; CI 14.48-18.03); PA (Mean 26.25; CI 24.41-28.10). As for gender-related effects, higher burnout rates were observed in the male workers, compared to the females, in all three domains: EE (Men: Mean 34.70; CI 29.90-39.50 -Women: Mean 31.03; CI 27.45-34.60); DP (Men: Mean 18.78; CI 15.98-21.59 -Women: Mean 14.90; CI 12.64-17.16) and PA (Men: Mean 24.54; CI 21.32-27.76 -Women: Mean 27.17; CI 24.90-29.44). The highest burnout rates, presented by the physiotherapists working in the profession for more than 20 years, were identified in the domain of EE (Mean: 35.30; CI 30.51-40.10) and in those with 10-15 years of experience, in the domains of DP (Mean: 18.31; CI 14.89-21.73) and PA (Mean: 23.97; CI 20.13-27.81). The highest rate of occupational burnout, reflected by the scores in all three domains (EE, DP, PA), was identified in Department I -Intensive Care and Anaesthesiology Department: EE - (Mean: 40.89, CI 35.27-46.52); DP - (Mean: 21.39, CI 17.90-24.88); and PA - (Mean: 23.07, CI 20.04-26.10), compared to the other departments. The subjects who rarely participated in courses or training programs showed the highest burnout rates (EE- Mean: 33.55, CI 29.33-37.77; DP- Mean: 16.71, CI 13.99-19.43; PA- Mean: 25.45, CI 22.47-28.43). CONCLUSIONS: Occupational burnout during the COVID-19 pandemic is noticeable among physiotherapists working in clinical departments. The current findings show high burnout rates in all three domains: emotional exhaustion (EE), depersonalization (DP) and personal accomplishment (PA). A comparative analysis of these findings with reference to related studies published before the pandemic shows that the burnout rates among physiotherapists may have significantly increased during the COVID-19 pandemic. However, given the scarcity of scientific evidence related to this specific problem in Poland and worldwide, it is necessary to continue research in occupational burnout affecting physiotherapists, particularly during the second wave of the pandemic, in order to gain a better understanding of the possible effects of social isolation and greater personal work-related health risks on the mental health of these medical professionals.

Role of body mass category in the development of faulty postures in school-age children from a rural area in south-eastern Poland: a cross-sectional study.

OBJECTIVE: The aim of the study was to assess the relationship between body mass index (BMI) and the incidence of abnormalities in selected parameters measured in the trunk area. DESIGN: Cross-sectional studies. SETTING: The research was conducted in a primary school in the Trzebownisko Municipality, a rural area in south-eastern Poland. PARTICIPANTS: A group of 464 children, ranging in age from 6 to 16 years (234 boys and 230 girls), was recruited to participate in the study. OUTCOME MEASURES: The examination of their body postures was conducted with the use of the Zebris system. Body mass was determined using a body mass analyser Tanita MC-780 MA. BMI was calculated based on the acquired data. RESULTS: It was noticed that the children with overweight and obesity tended to have an incorrect position of the shoulders and pelvis in comparison to children with normal body weight. It was found that greater body mass (higher BMI) coincided with a larger distance of the scapulae from the frontal plane (p=0.009). CONCLUSIONS: Increase in children's BMI produces adverse effects in the position of the shoulder blades, reflected by their greater distance from the frontal plane. Increase in BMI is not significantly related to the position of the shoulder joints or pelvis; however, the subjects with overweight or obesity presented a greater difference in the position of the shoulder joints and pelvis.

The Influence of Body Mass Composition on the Postural Characterization of School-Age Children and Adolescents.

INTRODUCTION: In recent years a significant increase in the percentage of children with postural problems has been observed. It is necessary to focus on preventing the phenomenon and on analysis of existing postural defects. AIM: The aim of this work was to assess the potential relationship between body mass composition and body posture in school-age children. MATERIAL AND METHODS: 464 school-age children ranging from 6 to 16 years (234 boys and 230 girls) were qualified for the study. Body mass composition was assessed using the analyzer Tanita MC 780 MA. Assessment of body posture was performed using Zebris system. All the results were analyzed with statistical methods. The accepted level of significance was p<0.05. RESULTS: Analyses of the disparities between the girls and the boys showed statistically significant differences in all of the body mass components and in obliquity of the pelvis/shoulder. The boys were characterized by higher contents of muscle tissue (p<0.001), bone tissue (p<0.001), fatless tissue (p<0.001), and body water (p<0.001) as well as a greater obliquity angle (p=0.018). The girls, on the other hand, were found with higher content of fatty tissue (p<0.001). The children with lower content of muscle tissue (p=0.030), fatless tissue (p=0.030), water (p=0.030), and higher content of fatty tissue measured in kg (p=0.027) were characterized by greater pelvic obliquity. CONCLUSION: The current study shows evidence that sex, as a strongly differentiating factor, determines body mass composition and the occurrence of suboptimal postures only in the pelvic area. Body mass composition differentiates body posture of the study group. The content of fatty tissue influences the occurrence of suboptimal postures in the scapula and pelvic area in the frontal plane. The content of muscle tissue is associated with faulty postures in the scapula and pelvic area in the frontal plane.

Bismuth film electrode and chloranilic acid as a new alternative for simple, fast and sensitive Ge(iv) quantification by adsorptive stripping voltammetry.

An analytical procedure regarding the voltammetric determination of germanium(iv) by adsorptive stripping voltammetry (AdSV) exploiting the in situ plated bismuth film electrode (BiFE) is described. The use of mercury free electrode as a working electrode is the first time proposed in AdSV germanium determination. The method is based on adsorptive accumulation of the Ge(iv)-chloranilic acid complex at a BiFE by a nonelectrochemical process followed by the cathodic stripping step. Experimental variables, including bismuth and chloranilic acid concentrations, deposition potential and time were carefully optimized. Under optimized conditions the cathodic stripping peak current was directly proportional to the concentration of Ge(iv) in the range from 3 × 10-9 to 1.5 × 10-7 mol L-1 with the correlation coefficient 0.997. Because the AdSV technique could be invalidated due to real samples matrix the influence of foreign ions, surface active substances, and humic substances on the Ge(iv) signal was precisely examined. The satisfying minimization of potential matrix interferences was also suggested. Analytical results of natural water samples analysis showed that the proposed procedure of trace germanium(iv) determination is suitable for direct environmental water analysis.

A Simple, Fast, and Inexpensive Simultaneous Determination of Trace Bismuth(III) and Lead(II) in Water Samples by Adsorptive Stripping Voltammetry.

A simple, fast, and inexpensive voltammetric method for the simultaneous determination of trace bismuth(III) and lead(II) using (Hg(Ag)FE) as a working electrode was optimized. For adsorptive stripping voltammetric determination of Bi(III) and Pb(II) in a single scan, the cupferron was applied as a complexing agent. Experimental conditions under which these elements can be simultaneously detected include 0.1 mol L-1 acetate buffer (pH = 4.6), 1 × 10-4 mol L-1 cupferron, accumulation potential -0.05 V, and accumulation time 30 s. The experiments were performed without deaeration of the solutions. The calibration graph was linear from 2 × 10-9 mol L-1 to 1 × 10-7 mol L-1 for the simultaneous presence of bismuth and lead. The detection limits for preconcentration time of 30 s were 6.7 × 10-10 mol L-1 and 8.8 × 10-10 mol L-1 for bismuth and lead, respectively. The application of this procedure was tested by analyzing certified reference material (SPS-WW1 Wastewater) and Lake Zemborzyce water (eastern areas of Poland).