Metallic nanoparticle actions on the outer layer structure and properties of Bacillus cereus and Staphylococcus epidermidis.

Although the antimicrobial activity of nanoparticles (NPs) penetrating inside the cell is widely recognised, the toxicity of large NPs (>10 nm) that cannot be translocated across bacterial membranes remains unclear. Therefore, this study was performed to elucidate the direct effects of Ag-NPs, Cu-NPs, ZnO-NPs and TiO2-NPs on relative membrane potential, permeability, hydrophobicity, structural changes within chemical compounds at the molecular level and the distribution of NPs on the surfaces of the bacteria Bacillus cereus and Staphylococcus epidermidis. Overall analysis of the results indicated the different impacts of individual NPs on the measured parameters in both strains depending on their type and concentration. B. cereus proved to be more resistant to the action of NPs than S. epidermidis. Generally, Cu-NPs showed the most substantial toxic effect on both strains; however, Ag-NPs exhibited negligible toxicity. All NPs had a strong affinity for cell surfaces and showed strain-dependent characteristic dispersion. ATR-FTIR analysis explained the distinctive interactions of NPs with bacterial functional groups, leading to macromolecular structural modifications. The results presented provide new and solid evidence for the current understanding of the interactions of metallic NPs with bacterial membranes.

Undesirable consequences of the metallic nanoparticles action on the properties and functioning of Escherichia coli, Bacillus cereus and Staphylococcus epidermidis membranes.

Controversial and inconsistent findings on the toxicity of metallic nanoparticles (NPs) against many bacteria are common in recorded studies; therefore, further advanced experimental work is needed to elucidate the mechanisms underlying nanotoxicity. This study deciphered the direct effects of Ag-NPs, Cu-NPs, ZnO-NPs and TiO2-NPs on membrane permeability, cytoplasmic leakage, ATP level, ATPase activity and fatty acid profiling of Escherichia coli, Bacillus cereus and Staphylococcus epidermidis as model microorganisms. A multifaceted analysis of all collected results indicated the different influences of individual NPs on the measured parameters depending on their type and concentration. Predominantly, membrane permeability was correlated with increased cytoplasmic leakage, reduced total ATP levels and ATPase activity. The established fatty acid profiles were unique and concerned various changes in the percentages of hydroxyl, cyclopropane, branched and unsaturated fatty acids. Decisively, E. coli was more susceptible to changes in measured parameters than B. cereus and S. epidermidis. Also, it was established that ZnO-NPs and Cu-NPs had a major differentiating impact on studied parameters. Additionally, bacterial cell imaging using scanning electron microscopy elucidated different NPs distributions on the cell surface. The presented results are believed to provide novel, valuable and accumulated knowledge in the understanding of NPs action on bacterial membranes.

Impedance Spectroscopy of Pr-Doped BaBi2Nb2O9 Aurivillius Ceramics.

Herein this study, the polycrystalline nature of the Aurivillius type structure is studied; primarily, the main objective is to observe the influence of dopant Pr3+ at the Ba2+-site of BaBi2Nb2O9 (BBN) ceramics. The ceramics under investigation were fabricated via the conventional solid-state reaction method. Scanning electron microscopy (SEM) and energy dispersion spectroscopy (EDS) techniques were used to analyse their morphological structure. It was found that the chemical composition of the ceramic samples corresponds well to the initial stoichiometry of the ceramic powders. An increase in praseodymium content caused a slight decrease in the average size of the ceramic grains. The obtained ceramic materials are described by a tetragonal structure with the space group I4/mmm. The electrical properties of the material have been studied using complex impedance spectroscopy methods in wide temperature and frequency ranges. The analysis of obtained results showed grains and grain boundaries contribute to conductive processes in the material. A possible 'hopping' mechanism for electrical transport processes in the system is evident from the analysis of results based on Joncher law.

The Effect of Pr Doping Contents on the Structural, Microstructure and Dielectric Properties of BaBi2Nb2O9 Aurivillius Ceramics.

Aurivillius BaBi2Nb2O9 and Ba1-xPrxBi2Nb2O9 ceramics were successfully synthesized by a simple solid state reaction method. Ceramics were prepared from reactants: Nb2O5, Bi2O3, BaCO3 and Pr2O3. The microstructure, structure, chemical composition, and dielectric properties of the obtained materials were examined. Dielectric properties were investigated in a wide range of temperatures (T = 20-500 °C) and frequencies (f = 0.1 kHz-1 MHz). The obtained ceramic materials belong to the group of layered perovskites, crystallizing in a tetragonal structure with the space group I4/mmm. Modification of the barium niobate compound with praseodymium ions influenced its dielectric properties and introducing a small concentration of the dopant ion causes a slight increase in the value of electric permittivity and shifts its maximum towards higher temperatures.

Fe-Doped Barium Lanthanum Titanate as a Competitor to Other Lead-Free Piezoelectric Ceramics.

Multiferroic solid solutions of Ba1-xLaxTi1-x/4O3 and iron (BLFT) were synthesized using the conventional mixed oxide method. The dependence of the piezoelectric coefficients on Fe content in BLFT ceramics was determined by the quasi-static and resonance method. The results indicate that 0.3 mol% addition of Fe3+ ions to the ceramic structure increased the value of the piezoelectric parameter d33 to the maximum of 159 pC/N. This puts BLFT ceramics among other good-quality and lead-free piezoelectric ceramics. A major enhancement of dielectric properties related to the manipulation of Fe content in the barium lanthanum titanate (BLT) ceramics system is reported as well.

Dielectric and Electric Properties of Ba0.996La0.004Ti0.999O3 Ceramics Doped with Europium and Hafnium Ions.

Lanthanum-modified BaTiO3 electroceramic materials have superior dielectric and piezoelectric properties. Ba0.996La0.004Ti0.999O3 (BLT4) seems to be a serious candidate for ultracondensator applications. This manuscript describes the results of hafnium and europium modification of BLT 4 ceramics. The pure and doped ceramic materials were synthesized by the conventional mixed oxide method. The microstructure of obtained samples was examined by scanning electron microscope. The investigations reveal strong correlations between the presence of admixture and the grain size, which was especially visible in the case of the hafnium dopant. The frequency and temperature dielectric characteristics measurements revealed a decrease in electric permittivity. Moreover, the impedance spectroscopy investigations showed severe changes in grains and grain-boundary resistivity, which was connected with changes in electric conductivity.

Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst.

Xanthan gum (XAN) is a widely used polysaccharide in various industries. Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen. The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%. A negative impact of entrapment was noted for strain Planococcus sp. S5, which showed a survival rate in the 93-51% range. To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA). XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules. Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive. This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora. Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.

Dielectric and Electrical Properties of BLT Ceramics Modified by Fe Ions.

The solid solution of the perovskite type structure Ba0.996La0.004Ti1-yFeyO3 (BLTF) for varying iron content (y = 0.1-0.4 mol.%) was obtained as a result of a solid state reaction using the conventional method. At room temperature (Tr < TC), the as-received ceramics reveals a single-phase, tetragonal structure and a P4mm space group. An increase in the iron content causes a slight decrease in the volume of the elementary cell. In addition, this admixture significantly reduces the maximum permittivity value (εm) and the shift of the phase transition temperature (TC) towards lower temperatures. The BLTF solid solution shows a classical phase transition and low values of dielectric loss tangent (tgδ), both at room temperature and in the phase transition area. The Curie-Weiss temperature (T0) and Curie constant (C) were also determined on the basis of the dielectric measurements results. The analysis of temperature changes in DC conductivity revealed presence of the positive temperature coefficient of resistivity (PTCR) effect in the phase transition area.

Impact of an Engineered Copper-Titanium Dioxide Nanocomposite and Parent Substrates on the Bacteria Viability, Antioxidant Enzymes and Fatty Acid Profiling.

Due to the systematic increase in the production of nanomaterials (NMs) and their applications in many areas of life, issues associated with their toxicity are inevitable. In particular, the performance of heterogeneous NMs, such as nanocomposites (NCs), is unpredictable as they may inherit the properties of their individual components. Therefore, the purpose of this work was to assess the biological activity of newly synthesized Cu/TiO2-NC and the parent nanoparticle substrates Cu-NPs and TiO2-NPs on the bacterial viability, antioxidant potential and fatty acid composition of the reference Escherichia coli and Bacillus subtilis strains. Based on the toxicological parameters, it was found that B. subtilis was more sensitive to NMs than E. coli. Furthermore, Cu/TiO2-NC and Cu-NPs had an opposite effect on both strains, while TiO2-NPs had a comparable mode of action. Simultaneously, the tested strains exhibited varied responses of the antioxidant enzymes after exposure to the NMs, with Cu-NPs having the strongest impact on their activity. The most considerable alternations in the fatty acid profiles were found after the bacteria were exposed to Cu/TiO2-NC and Cu-NPs. Microscopic images indicated distinct interactions of the NMs with the bacterial outer layers, especially in regard to B. subtilis. Cu/TiO2-NC generally proved to have less distinctive antimicrobial properties on B. subtilis than E. coli compared to its parent components. Presumably, the biocidal effects of the tested NMs can be attributed to the induction of oxidative stress, the release of metal ions and specific electrochemical interactions with the bacterial cells.

Correction: Wodecka-Dus, B. et al., Chemical and Physical Properties of the BLT4 Ultra Capacitor-A Suitable Material for Ultracapacitors Materials 2020, 13, 659.

The authors wish to make the following corrections to this paper [...].

Enhanced Degradation of Naproxen by Immobilization of Bacillus thuringiensis B1(2015b) on Loofah Sponge.

The naproxen-degrading bacterium Bacillus thuringiensis B1(2015b) was immobilised onto loofah sponge and introduced into lab-scale trickling filters. The trickling filters constructed for this study additionally contained stabilised microflora from a functioning wastewater treatment plant to assess the behavior of introduced immobilized biocatalyst in a fully functioning bioremediation system. The immobilised cells degraded naproxen (1 mg/L) faster in the presence of autochthonous microflora than in a monoculture trickling filter. There was also abundant colonization of the loofah sponges by the microorganisms from the system. Analysis of the influence of an acute, short-term naproxen exposure on the indigenous community revealed a significant drop in its diversity and qualitative composition. Bioaugmentation was also not neutral to the microflora. Introducing a new microorganism and increasing the removal of the pollutant caused changes in the microbial community structure and species composition. The incorporation of the immobilised B1(2015b) was successful and the introduced strain colonized the basic carrier in the trickling filter after the complete biodegradation of the naproxen. As a result, the bioremediation system could potentially be used to biodegrade naproxen in the future.

Dielectric and Impedance Studies of (Ba,Ca)TiO3 Ceramics Obtained from Mechanically Synthesized Powders.

Mechanochemical synthesis offers unique possibility of perovskite phase formation at ambient conditions that is very attractive (simplifies production, allows strict stoichiometry control and brings economic benefits). In this work the mechanochemical synthesis has been used for preparation ofBa1-xCaxTiO3 (0.2 ≤ x ≤ 0.3) powders from simple oxides. The 20 h milled powders have been uniaxially pressed and sintered in order to get the ceramic samples. The sample morphologies have been observed by scanning electron microscopy. Dielectric and impedance studies have been performed on ceramics. The obtained results indicate that the two mechanism of doping occurred. The first one is observed for the lower calcium concentration (below 0.3) and consists of the introduction of calcium ion into the A site of the perovskite structure. The second one is observed for the higher calcium concentration (equal 0.3). In this case the calcium ions partially occupies the B site in the perovskite structure. Both cases have different influence on the final properties of the ceramics because they induce different defects.