ABSTRACT
The fructofuranosidases (EC 3.2.1.26) of Aspergillus niger St-0018 and A. foetidus St-0194 were used to produce fructooligosaccharides (FOS) under periodic and continuous conditions. The incorporation of cells into calcium alginate gel gave the most efficient immobilized biocatalysts. The feasibility of transforming residual sucrose into palatinose and trehalulose using isomaltulose synthase (EC 5.4.99.11) was demonstrated.
Subject(s)
Isomaltose/analogs & derivatives , Oligosaccharides/biosynthesis , Sucrose/metabolism , Trehalose/metabolism , beta-Fructofuranosidase/biosynthesis , Aspergillus/enzymology , Catalysis , Disaccharides/biosynthesis , Gammaproteobacteria/enzymology , Intramolecular Transferases/metabolism , Isomaltose/biosynthesisABSTRACT
Cyclodextrin glucanotransferases (CGTase, EC 2.4.1.19) produced by mesophilic, thermophilic, and halophilic bacilli, as well as maltase (EC 3.2.1.20) produced by various strains of Saccharomyces cerevisiae have been applied for transglycosylation of L-ascorbic acid using starch, maltodextrin, gamma-cyclodextrin, and maltose as donors of glucosyl residue. The CGTases produced by thermophilic strains are the most efficient. The degree of transglucosylation is more than 60%.
