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1.
PLoS Genet ; 9(3): e1003343, 2013.
Article in English | MEDLINE | ID: mdl-23505388

ABSTRACT

Protein arginine methyltransferase 4 (PRMT4)-dependent methylation of arginine residues in histones and other chromatin-associated proteins plays an important role in the regulation of gene expression. However, the exact mechanism of how PRMT4 activates transcription remains elusive. Here, we identify the chromatin remodeller Mi2α as a novel interaction partner of PRMT4. PRMT4 binds Mi2α and its close relative Mi2ß, but not the other components of the repressive Mi2-containing NuRD complex. In the search for the biological role of this interaction, we find that PRMT4 and Mi2α/ß interact with the transcription factor c-Myb and cooperatively coactivate c-Myb target gene expression in haematopoietic cell lines. This coactivation requires the methyltransferase and ATPase activity of PRMT4 and Mi2, respectively. Chromatin immunoprecipitation analysis shows that c-Myb target genes are direct transcriptional targets of PRMT4 and Mi2. Knockdown of PRMT4 or Mi2α/ß in haematopoietic cells of the erythroid lineage results in diminished transcriptional induction of c-Myb target genes, attenuated cell growth and survival, and deregulated differentiation resembling the effects caused by c-Myb depletion. These findings reveal an important and so far unknown connection between PRMT4 and the chromatin remodeller Mi2 in c-Myb signalling.


Subject(s)
Autoantigens , Chromatin/genetics , Mi-2 Nucleosome Remodeling and Deacetylase Complex , Protein-Arginine N-Methyltransferases , Proto-Oncogene Proteins c-myb , Autoantigens/genetics , Autoantigens/metabolism , Bone Marrow Cells , Cell Line , Chromatin Assembly and Disassembly/genetics , Gene Expression Regulation , Histones/genetics , Histones/metabolism , Humans , Methylation , Mi-2 Nucleosome Remodeling and Deacetylase Complex/genetics , Mi-2 Nucleosome Remodeling and Deacetylase Complex/metabolism , Protein-Arginine N-Methyltransferases/genetics , Protein-Arginine N-Methyltransferases/metabolism , Proto-Oncogene Proteins c-myb/genetics , Proto-Oncogene Proteins c-myb/metabolism , Transcriptional Activation
2.
Oral Oncol ; 45(10): e140-9, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19502098

ABSTRACT

The peptidyl-prolyl cis/trans isomerase Pin1 has been implicated in malignant transformation in multiple studies, however, little is known about its potential impact in head and neck cancer. This study evaluates the role of Pin1 in head and neck squamous cell carcinomas (HNSCCs). Pin1 expression and level of phosphorylation was evaluated by Western blot analysis and 2D-gel-electrophoresis. Pin1 was inhibited with juglone (5-hydroxy-1,4-naphthalenedione) or Pin1 specific siRNA and its influence on cell cycle checkpoint distribution was assessed by FACS analysis. Pin1 overexpression was found in HNSCC tissues and cell lines. 2D-gel-electrophoresis data pointed to Pin1 being hypophosphorylated in HNSCC cells which is consistent with overactivation of this rotamase. Inhibition of HNSCC cells with juglone or Pin1 siRNA induced the cell cycle inhibitor p21(WAF1/Cip1) with a concomitant reduction of cells in G2/M and an increased fraction of cells with fragmented DNA. Cell death did not correlate with significant levels of apoptosis in Pin1 depleted HNSCC cells. In summary, the data shows that Pin1 is overexpressed and hypophosphorylated in HNSCC, and that inhibition of Pin1 blocks cell cycle progression and triggers tumor cell death. Pin1 therefore could represent a new target for the development of improved HNSCC targeting drugs.


Subject(s)
Carcinoma, Squamous Cell/metabolism , Head and Neck Neoplasms/metabolism , Neoplasm Proteins/metabolism , Peptidylprolyl Isomerase/metabolism , Adult , Aged , Aged, 80 and over , Alkaline Phosphatase/pharmacology , Apoptosis , Blotting, Western , Case-Control Studies , Cell Cycle/physiology , Cell Proliferation , Cyclin-Dependent Kinase Inhibitor p21/metabolism , Electrophoresis, Gel, Two-Dimensional , Enzyme Inhibitors/pharmacology , Female , Humans , Male , Middle Aged , NIMA-Interacting Peptidylprolyl Isomerase , Naphthoquinones/pharmacology , Peptidylprolyl Isomerase/antagonists & inhibitors , Phosphorylation , RNA, Small Interfering/pharmacology , Up-Regulation
3.
EMBO J ; 26(15): 3686-98, 2007 Aug 08.
Article in English | MEDLINE | ID: mdl-17641685

ABSTRACT

The peroxisome proliferator-activated receptor-beta (PPARbeta) has been implicated in tumorigenesis, but its precise role remains unclear. Here, we show that the growth of syngeneic Pparb wild-type tumors is impaired in Pparb(-/-) mice, concomitant with a diminished blood flow and an abundance of hyperplastic microvascular structures. Matrigel plugs containing pro-angiogenic growth factors harbor increased numbers of morphologically immature, proliferating endothelial cells in Pparb(-/-) mice, and retroviral transduction of Pparb triggers microvessel maturation. We have identified the Cdkn1c gene encoding the cell cycle inhibitor p57(Kip2) as a PPARbeta target gene and a mediator of the PPARbeta-mediated inhibition of cell proliferation, which provides a possible mechanistic explanation for the observed tumor endothelial hyperplasia and deregulation of tumor angiogenesis in Pparb(-/-) mice. Our data point to an unexpected essential role for PPARbeta in constraining tumor endothelial cell proliferation to allow for the formation of functional tumor microvessels.


Subject(s)
Down-Regulation , Neoplasms, Experimental/blood supply , Neovascularization, Pathologic , PPAR-beta/physiology , Animals , Cell Proliferation , Cyclin-Dependent Kinase Inhibitor p57/metabolism , Endothelium, Vascular/pathology , Magnetic Resonance Imaging , Mice , Mice, Knockout , Neoplasms, Experimental/pathology , Oligonucleotide Array Sequence Analysis , PPAR-beta/genetics , PPAR-beta/metabolism
4.
Proteomics ; 7(8): 1208-16, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17380536

ABSTRACT

The peroxisome proliferator activated receptor-beta (PPARbeta) plays an essential role in lipid metabolism, immune modulation, differentiation and cell proliferation. There is also strong evidence for a function in oncogenesis and tumor vascularization, but the underlying molecular mechanisms remain elusive. In the present study, we have used fibroblasts derived from Pparb wild-type and null mice to determine by 2-DE and PMF analysis the contribution of PPARbeta to the protein profile of fibroblasts. Thirty-one differentially expressed proteins of different functional categories were identified. For at least two proteins a role in tumorigenesis and/or tumor vascularization has previously been reported: while the calcium intracellular channel-4 (CLIC4) was expressed at lower levels in Pparb null cells, expression of the cellular retinol binding protein 1 (CRBP1) was enhanced. Clic4 and Crbp1 gene expression patterns observed in different experimental settings in vitro and in vivo confirmed the proteomics data. Our findings indicate that the expression of a defined set of proteins is altered in fibroblasts and endothelial cells from Pparb null mice, that this is due to aberrant gene regulation, and that the altered expression of these proteins is consistent with the tumor vascularization phenotype of Pparb null mice.


Subject(s)
Fibroblasts/chemistry , Gene Targeting , PPAR delta/genetics , PPAR-beta/genetics , Proteome/analysis , Animals , Cells, Cultured , Chloride Channels/genetics , Chloride Channels/metabolism , Electrophoresis, Gel, Two-Dimensional , Endothelial Cells/cytology , Endothelial Cells/metabolism , Humans , Mice , Mice, Inbred C57BL , Mice, Knockout , Molecular Sequence Data , PPAR delta/metabolism , PPAR-beta/metabolism , Retinol-Binding Proteins/genetics , Retinol-Binding Proteins/metabolism , Retinol-Binding Proteins, Cellular
5.
Adv Exp Med Biol ; 522: 47-57, 2003.
Article in English | MEDLINE | ID: mdl-12674210

ABSTRACT

Our in vitro data indicate that peripheral blood monocytes or monocyte-derived immature dendritic cells under appropriate culture conditions transdifferentiate into endothelial-like cells (ELC), which are characterized by the expression of endothelial markers and the formation of tube-like structures. Dependent on the culture conditions a mixed macrophage/endothelial or an endothelial phenotype could be induced. A similar pattern of development could be seen in CD14+ monocyte-derived ELC and ELC grown from CD34+ precursor cells or from dendritic cells generated from CD34+ cells. These in vitro data suggest that monocytes are precursors of different subgroups of endothelial cells and that the formation of endothelial cells from CD34+ progenitor cells follows a similar pathway possibly via the monocyte and/or the immature dendritic cell.


Subject(s)
Cell Differentiation/physiology , Cell Lineage/physiology , Cells, Cultured/cytology , Dendritic Cells/cytology , Monocytes/cytology , Neovascularization, Physiologic/physiology , Stem Cells/cytology , Animals , Antigens, CD34/immunology , Cells, Cultured/immunology , Cells, Cultured/metabolism , Dendritic Cells/immunology , Dendritic Cells/metabolism , Humans , In Vitro Techniques , Lipopolysaccharide Receptors/immunology , Monocytes/immunology , Monocytes/metabolism , Stem Cells/immunology , Stem Cells/metabolism
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