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1.
Oncogene ; 34(41): 5277-87, 2015 Oct 08.
Article in English | MEDLINE | ID: mdl-25659577

ABSTRACT

A truncation mutant of the epidermal growth factor receptor, EGFRvIII, is commonly expressed in glioma, an incurable brain cancer. EGFRvIII is tumorigenic, in part, through its transactivation of other receptor tyrosine kinases (RTKs). Preventing the effects of this transactivation could form part of an effective therapy for glioma; however, the mechanism by which the transactivation occurs is unknown. Focusing on the RTK MET, we show that MET transactivation in U87MG human glioma cells in vitro is proportional to EGFRvIII activity and involves MET heterodimerization associated with a focal adhesion kinase (FAK) scaffold. The transactivation of certain other RTKs was, however, independent of FAK. Simultaneously targeting EGFRvIII (with panitumumab) and the transactivated RTKs themselves (with motesanib) in an intracranial mouse model of glioma resulted in significantly greater survival than with either agent alone, indicating that cotargeting these RTKs has potent antitumor efficacy and providing a strategy for treating EGFRvIII-expressing gliomas, which are usually refractory to treatment.


Subject(s)
Brain Neoplasms/metabolism , ErbB Receptors/physiology , Glioma/metabolism , Transcriptional Activation , Analgesics/pharmacology , Animals , Antibodies, Monoclonal/pharmacology , Apoptosis , Brain Neoplasms/drug therapy , Brain Neoplasms/genetics , Cell Line, Tumor , ErbB Receptors/antagonists & inhibitors , Female , Focal Adhesion Kinase 1/metabolism , Glioma/drug therapy , Glioma/genetics , Indoles/pharmacology , Mice, Inbred BALB C , Niacinamide/analogs & derivatives , Niacinamide/pharmacology , Oligonucleotides , Panitumumab , Protein Kinase Inhibitors/pharmacology , Proto-Oncogene Proteins c-met/genetics , Proto-Oncogene Proteins c-met/metabolism , Receptor Protein-Tyrosine Kinases/antagonists & inhibitors , Receptor Protein-Tyrosine Kinases/metabolism , Signal Transduction , Xenograft Model Antitumor Assays
2.
Oncogene ; 34(13): 1658-66, 2015 Mar 26.
Article in English | MEDLINE | ID: mdl-24747966

ABSTRACT

A feature of many gliomas is the amplification of the epidermal growth factor receptor (EGFR), resulting in its overexpression. Missense mutations or deletions within the extracellular domain are associated with this amplification and can lead to constitutive activation of the receptor, with the Domain I/II deletion, EGFRvIII, being the most common. These changes have also been associated with increased sensitivity to EGFR inhibition using small molecule inhibitors. We have expressed, in human glioma cells, EGFR containing four glioma-specific EGFR missense mutations within Domain IV (C620Y, C624F, C628Y and C636Y) to analyze their biological properties and sensitivity to EGFR inhibition. One of these mutants, C620Y, exhibited an enhanced basal phosphorylation, which was partially dependent on an EGFR-ligand autocrine loop. All Domain IV mutants responded equally as well as wildtype EGFR (wtEGFR) to ligand stimulation. Biochemical analysis revealed that a pre-formed, disulfide-bonded dimer associated with these mutations was underglycosylated, inactive and cytoplasmically retained. Ligand stimulation resulted in the formation of a tyrosine-phosphorylated, disulfide-bonded dimer for all Domain IV mutants but not for wtEGFR. Following treatment with the next-generation, irreversible pan-ErbB inhibitor dacomitinib, the C620Y, C624F and EGFRvIII mutants were inactivated, covalently dimerized and were retained in the cytoplasm, resulting in cell-surface receptor loss and, for C620Y and C624F, decreased binding of EGF. Dacomitinib treatment significantly reduced the in vivo growth of human glioma xenografts bearing C620Y, but not wtEGFR. Collectively, these data indicate that the unique biochemical traits of Domain IV EGFR cysteine mutants can be exploited for enhanced sensitivity to EGFR small molecule inhibitors, with potential clinical applications.


Subject(s)
ErbB Receptors/genetics , Glioma/drug therapy , Mutation , Protein Multimerization , Quinazolinones/therapeutic use , Animals , Cell Line, Tumor , Cysteine , ErbB Receptors/antagonists & inhibitors , ErbB Receptors/chemistry , Female , Glioma/genetics , Glioma/pathology , Humans , Ligands , Mice , Mice, Inbred BALB C , Phosphorylation , Protein Structure, Tertiary , Quinazolinones/pharmacology
3.
J Thromb Haemost ; 7(10): 1688-95, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19656282

ABSTRACT

BACKGROUND: Coagulation is a highly regulated process where the ability to prevent blood loss after injury is balanced against the maintenance of blood fluidity. Thrombin is at the center of this balancing act. It is the critical enzyme for producing and stabilizing a clot, but when complexed with thrombomodulin (TM) it is converted to a powerful anticoagulant. Another cofactor that may play a role in determining thrombin function is the monovalent cation Na(+). Its apparent affinity suggests that half of the thrombin generated is in a Na(+)-free 'slow' state and half is in a Na(+)-coordinated 'fast' state. While slow thrombin is a poor procoagulant enzyme, when complexed to TM it is an effective anticoagulant. METHODS: To better understand this molecular transformation we solved a 2.4 A structure of thrombin complexed with EGF domains 4-6 of TM in the absence of Na(+) and other cofactors or inhibitors. RESULTS: We find that TM binds as previously observed, and that the thrombin component resembles structures of the fast form. The Na(+) binding loop is observed in a conformation identical to the Na(+)-bound form, with conserved water molecules compensating for the missing ion. Using the fluorescent probe p-aminobenzamidine we show that activation of slow thrombin by TM principally involves the opening of the primary specificity pocket. CONCLUSIONS: These data show that TM binding alters the conformation of thrombin in a similar manner as Na(+) coordination, resulting in an ordering of the Na(+) binding loop and an opening of the adjacent S1 pocket. We conclude that other, more subtle subsite changes are unlikely to influence thrombin specificity toward macromolecular substrates.


Subject(s)
Thrombin/metabolism , Thrombomodulin/metabolism , Allosteric Regulation , Amino Acid Substitution , Binding Sites , Catalytic Domain , Crystallography, X-Ray , Humans , Hydrophobic and Hydrophilic Interactions , Models, Molecular , Mutation, Missense , Protein Binding , Protein Conformation , Protein Interaction Mapping , Protein Structure, Tertiary , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Sodium/metabolism , Spectrometry, Fluorescence , Thrombin/chemistry , Thrombin/genetics , Thrombomodulin/chemistry
4.
Reprod Domest Anim ; 43 Suppl 2: 186-92, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18638122

ABSTRACT

Using recombinant gonadotropins for assisted reproduction of domestic species is still in its infancy. Yet, the purity, potency and pathogen-free nature of recombinant gonadotropins make them attractive alternatives to tissue-derived gonadotropic agents. In this study, the authors summarize the work to date using recombinant gonadotropins to enhance the - fertility of domestic animals and they discussed their recent studies examining the biopotency of single chain analogues of human gonadotropins. In these studies, single chain analogues of follicle stimulating hormone (Fc alpha), chorionic gonadotropin (CG beta alpha) or a gonadotropin construct with dual activity (FcCG beta alpha) were administered to sheep pre-treated with antisera directed against GnRH. Ovulation was induced 3 days after analogue administration using hCG (1000 IU, iv). Although Fc alpha or CG beta alpha alone induced only modest oestradiol production during the pre-hCG period, serum concentrations of oestradiol were markedly increased (p < 0.05) 3 days after administration of FcCG beta alpha or the Fc alpha + CG beta alpha combination. Final ovarian weight was significantly increased (p < 0.05) in animals receiving Fc alpha, Fc alpha + CG beta alpha or FcCG beta alpha. Collectively, these observations demonstrate that the single chain analogues of the human gonadotropins are active in sheep.


Subject(s)
Gonadotropin-Releasing Hormone/therapeutic use , Gonadotropins/physiology , Gonadotropins/therapeutic use , Ovulation Induction/veterinary , Sheep/physiology , Animals , Animals, Domestic , Female , Gonadotropin-Releasing Hormone/analogs & derivatives , Gonadotropin-Releasing Hormone/antagonists & inhibitors , Ovarian Follicle/physiology , Ovulation/physiology , Ovulation Induction/methods , Recombinant Proteins/therapeutic use , Reproductive Techniques, Assisted/veterinary
5.
J Anim Sci ; 86(5): 1124-31, 2008 May.
Article in English | MEDLINE | ID: mdl-18272858

ABSTRACT

Three experiments were designed to test the effect of dietary restriction on clearance of 17beta-estradiol (E(2)) in sheep. A preliminary experiment examined the effect of a 4-d fast on the rate of E(2) clearance in wethers. The second experiment tested the hypothesis that either long-term restriction (7 wk) or a 5-d fast would increase steroid-binding capacity of serum by increasing the concentration of sex hormone-binding globulin (SHBG) in the blood of ovariectomized ewes. In Exp. 3, we hypothesized that nutrition-dependent regulation of E(2) clearance by the liver would result in divergence in biliary extraction of E(2) in fed and fasted wethers receiving comparable levels of exogenous E(2). A marked difference in E(2) clearance between fed and fasted wethers was noted in the preliminary study. Relative to ad libitumfed wethers, a 4-d fast decreased E(2) clearance by 52%. Serum concentrations of SHBG were increased in long-term energy-restricted and fasted ewes, relative to the concentration in maintenancefed ewes (P = 0.015). Furthermore, a 5-d fast nearly doubled serum steroid-binding capacity in wethers. The E(2) concentration in bile was 2 times greater in fasted than in fed wethers. This fasting-dependent increase in biliary E(2) may be reflective of the increased serum E(2) in fasted animals, because each 1 pg/mL increase in serum E(2) increased bile E(2) by 0.86 +/- 0.12 pg/mL, independent of nutrition (P = 0.002). Our results demonstrate that the rate of clearance of E(2) is decreased during nutritional restriction. Additionally, these data indicate that altered SHBG expression, enterohepatic recirculation, or both are involved in the decreased E(2) clearance during dietary restriction.


Subject(s)
Animal Nutritional Physiological Phenomena , Diet, Reducing/veterinary , Estradiol/pharmacokinetics , Sex Hormone-Binding Globulin/metabolism , Sheep/metabolism , Animals , Fasting/blood , Fasting/physiology , Female , Liver/metabolism , Male , Orchiectomy , Ovariectomy/veterinary , Random Allocation , Sheep/blood
6.
Gen Comp Endocrinol ; 147(3): 384-90, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16630617

ABSTRACT

Interrenal function and the magnitude of the stress response were assessed in green sturgeon (Acipenser medirostris) passively immunized with antisera directed against adrenocorticotropic hormone (ACTH). The nucleotide sequence encoding ACTH was determined using reverse transcriptase polymerase chain reaction (RT-PCR). We identified two isoforms of ACTH that differ at a single site (position 26) in the 39 AA peptide. Both forms of green sturgeon ACTH (gsACTH1-39) display 100% homology with both sequences of white sturgeon ACTH (wsACTH1-39). The N-terminal portion of gsACTH also shares absolute identity with the comparable portion of human ACTH (hACTH). However, we identified considerable sequence divergence in the C-terminal domain between gsACTH and hACTH. Species-specific anti-ACTH sera were generated by vaccinating sheep against the C-terminal portion of gsACTH (gsACTH26-39). The peptide was covalently linked to a carrier protein (keyhole-limpet-hemocyanin [KLH]) to further enhance its immunogenicity. The anti-gsACTH sera recognized gsACTH1-39 and the immunogenic peptide (gsACTH26-39), but did not interact with hACTH1-39. To assess the impact of the antisera, fish were passively immunized with anti-gsACTH26-39 sera or anti-KLH sera and challenged with a hACTH1-39 injection on day 1 followed by a 1-min air emersion stressor on day 2. The magnitude and duration of the secretory response induced by hACTH did not differ (P > .05) between groups. Conversely, the magnitude of cortisol secretion induced by air emersion was significantly attenuated (P < .05) in fish passively immunized against gsACTH26-39. Collectively, these data demonstrate that the targeted antisera used in this study can discriminate between mammalian and green sturgeon ACTH and moderate the in vivo response to a stressor.


Subject(s)
Adrenocorticotropic Hormone/immunology , Fishes/physiology , Immune Sera/pharmacology , Kidney/physiology , Stress, Physiological/metabolism , Adrenocorticotropic Hormone/genetics , Amino Acid Sequence , Animals , Antibody Specificity , Fishes/immunology , Humans , Hydrocortisone/blood , Molecular Sequence Data , Pro-Opiomelanocortin/genetics , Protein Structure, Tertiary , Species Specificity
7.
Physiol Biochem Zool ; 78(4): 599-609, 2005.
Article in English | MEDLINE | ID: mdl-15957114

ABSTRACT

Metabolic scope for activity (MSA) and critical swimming velocity (U(crit)) were measured in green sturgeon exposed to two stressors daily for 28 consecutive days. The results were compared with unstressed fish in an effort to measure the "cost" of chronic stress. Chronic stress was simulated by exposing fish to a randomized order of acute stressors: a 5-min chasing stressor, a 10-min water depth reduction stressor, or a 5-min confinement stressor. The acute cortisol response to each stressor was initially determined, and the maintenance of that response was verified in 7-d intervals during the chronic stress regime. Exposure to the chronic stress regime resulted in a 25% reduction of MSA caused by significantly increased maintenance metabolic rate (0.27+/-0.01 vs. 0.19+/-0.02 mg O(2) h(-1) g(-1), chronic and control fish, respectively) but did not affect the U(crit) of sturgeon. In addition, a 50% reduction in liver glycogen levels and a twofold increase of resting plasma glucose levels were measured in chronically stressed fish. We conclude that our chronic stress regime resulted in a significant maintenance cost to green sturgeon, possibly because of their inability to habituate to the stressors, but did not decrease their swimming performance.


Subject(s)
Energy Metabolism/physiology , Fishes/physiology , Stress, Physiological/physiopathology , Swimming/physiology , Analysis of Variance , Animals , Blood Glucose , California , Glycogen/metabolism , Hydrocortisone/blood , Liver/metabolism , Time Factors
8.
J Thromb Haemost ; 1(5): 1024-7, 2003 May.
Article in English | MEDLINE | ID: mdl-12871372

ABSTRACT

Thrombotic disorders can lead to uncontrolled thrombin generation and clot formation within the circulatory system leading to vascular thrombosis. Direct inhibitors of thrombin have been developed and tested in clinical trials for the treatment of a variety of these thrombotic disorders. The bleeding complications observed during these trials have raised questions about their clinical use. The development of a computer-based model of coagulation using the kinetic rates of individual reactions and concentrations of the constituents involved in each reaction within blood has made it possible to study coagulation pathologies in silico. We present an extension of our initial model of coagulation to include several specific thrombin inhibitors. Using this model we have studied the effect of a variety of inhibitors on thrombin generation and compared these results with the clinically observed data. The data suggest that numerical models will be useful in predicting the effectiveness of inhibitors of coagulation.


Subject(s)
Anticoagulants/pharmacology , Computer Simulation , Glycine/analogs & derivatives , Hirudins/analogs & derivatives , Thrombin/antagonists & inhibitors , Arginine/analogs & derivatives , Azetidines , Benzylamines , Blood Coagulation/drug effects , Dose-Response Relationship, Drug , Glycine/pharmacology , Hirudins/pharmacology , Humans , Kinetics , Models, Cardiovascular , Oligopeptides/pharmacology , Peptide Fragments/pharmacology , Pipecolic Acids/pharmacology , Recombinant Proteins/pharmacology , Sulfonamides
9.
Comp Biochem Physiol A Mol Integr Physiol ; 135(2): 291-302, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12781829

ABSTRACT

The effects of time of day and water temperature on the acute physiological stress response were investigated in young-of-the-year green sturgeon (Acipenser medirostris). The response to a 1-min air-emersion stressor was assessed during the day (08.00 h) and at night (20.00 h), as well as after acclimation to either 11 degrees C or 19 degrees C. Blood samples were collected prior to stress and at several times after exposure to the stressor, and plasma concentrations of cortisol, lactate, and glucose were determined. The magnitudes of cortisol (19.1 ng ml(-1) vs. 4.9 ng ml(-1)) and lactate (190.6 mg l(-1) vs. 166.7 mg l(-1)) were significantly higher in fish stressed at night when compared with the day. There were no significant differences in glucose levels between time periods. Although, acclimation temperature did not affect peak cortisol concentrations (56.7 and 50.3 ng ml(-1) at 11 degrees C and 19 degrees C, respectively), the duration of the response was significantly extended at 11 degrees C. Post-stressor lactate increases were similar between temperature groups, but at 11 degrees C post-stressor glucose levels were significantly increased through 6 h, suggesting stressor-induced glycogenolysis and gluconeogenesis or decreased glucose utilization. These data demonstrate that the physiological stress response in green sturgeon is modified by both time of day and temperature.


Subject(s)
Fishes/physiology , Oxidative Stress/physiology , Temperature , Animals , Blood Glucose/analysis , Circadian Rhythm , Environment Design , Fishes/blood , Gluconeogenesis/physiology , Glycogen Storage Disease/metabolism , Hydrocortisone/blood , Lactates/blood , Water
10.
J Anim Sci ; 81(2): 411-5, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12643484

ABSTRACT

The purpose of this research was to compare the frequency of aggressive behavior's in beef bulls actively immunized against gonadotropin-releasing hormone relative to contemporary nonimmunized control bulls and surgically castrated steers. Eight males were assigned to each ofthese treatments in each of 4 yr. Immunized males were treated with a GnRH-keyhole-limpet hemocyanin (KLH) conjugate at approximately 4 mo of age. A secondary (booster) immunization was administered at 12 mo. Steers were castrated at 4 mo of age. Animals in each treatment in each year were housed as a single group prior to testing. At approximately 16 mo of age, each group of eight animals was placed in a 10- x 16-m enclosure for 20 min on five occasions at 2 to 3 d intervals. An observer recorded butts initiated by each animal as well as participation in bouts of sparring. Relative to control bulls, immunocastration reduced the frequency of butts initiated (P < 0.05) and participation in sparring bouts (P < 0.05) to levels typically observed in steers (P > 0.05). These observations indicate that active immunization against GnRH reduces the incidence of aggressive behavior in male beef cattle and are consistent with our postulate that immunoneutralization of GnRH is an effective alternative to surgical castration in the management of beef cattle.


Subject(s)
Aggression , Behavior, Animal , Cattle/physiology , Gonadotropin-Releasing Hormone/immunology , Orchiectomy/veterinary , Animals , Cattle/growth & development , Cattle/immunology , Immunity, Active/immunology , Immunization, Secondary , Male , Random Allocation , Testosterone/blood , Vaccination/veterinary , Weight Gain
11.
Anim Reprod Sci ; 71(3-4): 203-15, 2002 Jun 17.
Article in English | MEDLINE | ID: mdl-12047929

ABSTRACT

The effect of immunoneutralization of gonadotropin-releasing hormone (GnRH) on serum concentrations of testosterone and sexual behavior was evaluated in sexually mature male sheep. In Experiment 1, GnRH1 rams (n=16) were passively immunized against GnRH (300 ml antiserum), control rams were either passively immunized against keyhole limpet hemocyanin (KLH, n=15) or surgically castrated (Wethers1, n=4). Sexual performance of the rams was assessed weekly for 3 weeks before and 6 weeks after immunization, using ovarihystertomized ewes actively immunized against GnRH. Experiment 2 evaluated the effects of repeated immunization. Rams were immunized with two aliquots (400 and 300 ml, respectively) of anti-GnRH sera (GnRH, n=5) or normal sheep serum (NSS, n=4), 2 weeks apart. Surgically castrated animals were used as a second control group (Wethers2). Administration of anti-GnRH sera, but neither anti-KLH nor NSS sera, resulted in marked reduction (P<0.05) in serum concentrations of testosterone. Sexual behavior was not consistently affected by administration of one aliquot of anti-GnRH sera, however repeated immunizations resulted in more persistent reduction in serum concentrations of testosterone and more consistent suppression of sexual behavior.


Subject(s)
Antibodies/blood , Gonadotropin-Releasing Hormone/immunology , Immunization, Passive , Sexual Behavior, Animal , Sheep/physiology , Testosterone/blood , Animals , Female , Gonadotropin-Releasing Hormone/physiology , Kinetics , Male , Orchiectomy/veterinary , Ovariectomy/veterinary
12.
Protein Expr Purif ; 23(1): 142-50, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11570856

ABSTRACT

Attachment of a hexa-His tag is a common strategy in recombinant protein production. The use of such a tag greatly simplifies the purification of the protein from the complex mixture of other proteins in the media or cell extract. We describe the production of two recombinant nonglycosylated human serum transferrins (hTF-NG), containing a factor Xa cleavage site and a hexa-His tag at their carboxyl-terminal ends. One of the constructs comprises the entire coding region for hTF (residues 1-679), while the other lacks the final three carboxyl-terminal amino acids. After insertion of the His-tagged hTFs into the pNUT vector, transfection into baby hamster kidney (BHK) cells, and selection with methotrexate, the secreted recombinant proteins were isolated from the tissue culture medium. Average maximum expression levels of the His-tagged hTFs were about 40 mg/L compared to an average maximum of 50 mg/L for hTF-NG. The first step of purification involved an anion exchange column. The second step utilized a Poros metal chelate column preloaded with copper from which the His-tagged sample was eluted with a linear imidazole gradient. The His-tagged hTFs were characterized and compared to both recombinant hTF-NG and glycosylated hTF from human serum. The identity of each of the His-tagged hTFs constructs was verified by electrospray mass spectroscopy. In summary, the His-tagged hTF constructs simplify the purification of these metal-binding proteins with minimal effects on many of their physical properties. The His-tagged hTFs share many features common to hTF, including reversible iron binding, reactivity with a monoclonal antibody, and presence as a monomer in solution.


Subject(s)
Cloning, Molecular/methods , Histidine , Transferrin/biosynthesis , Affinity Labels , Animals , Antibodies, Monoclonal/metabolism , Cell Line , Chromatography , Cricetinae , Glycosylation , Humans , Iron/metabolism , Protein Binding , Recombinant Proteins/biosynthesis , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Spectrum Analysis , Transfection , Transferrin/isolation & purification , Transferrin/metabolism
13.
Biochemistry ; 40(30): 8930-9, 2001 Jul 31.
Article in English | MEDLINE | ID: mdl-11467954

ABSTRACT

Murine and human epidermal growth factor receptors (EGFRs) bind human EGF (hEGF), mouse EGF (mEGF), and human transforming growth factor alpha (hTGF-alpha) with high affinity despite the significant differences in the amino acid sequences of the ligands and the receptors. In contrast, the chicken EGFR can discriminate between mEGF (and hEGF) and hTGF-alpha and binds the EGFs with approximately 100-fold lower affinity. The regions responsible for this poor binding are known to be Arg(45) in hEGF and the L2 domain in the chicken EGFR. In this study we have produced a truncated form of the hEGFR ectodomain comprising residues 1-501 (sEGFR501), which, unlike the full-length hEGFR ectodomain (residues 1-621, sEGFR621), binds hEGF and hTGF-alpha with high affinity (K(D) = 13-21 and 35-40 nM, respectively). sEGFR501 was a competitive inhibitor of EGF-stimulated mitogenesis, being almost 10-fold more effective than the full-length EGFR ectodomain and three times more potent than the neutralizing anti-EGFR monoclonal antibody Mab528. Analytical ultracentrifugation showed that the primary EGF binding sites on sEGFR501 were saturated at an equimolar ratio of ligand and receptor, leading to the formation of a 2:2 EGF:sEGFR501 dimer complex. We have used sEGFR501 to generate three mutants with single position substitutions at Glu(367), Gly(441), or Glu(472) to Lys, the residue found in the corresponding positions in the chicken EGFR. All three mutants bound hTGF-alpha and were recognized by Mab528. However, mutant Gly(441)Lys showed markedly reduced binding to hEGF, implicating Gly(441), in the L2 domain, as part of the binding site that recognizes Arg(45) of hEGF.


Subject(s)
Epidermal Growth Factor/metabolism , ErbB Receptors/genetics , ErbB Receptors/metabolism , Peptide Fragments/genetics , Peptide Fragments/metabolism , Sequence Deletion , Animals , Binding, Competitive/genetics , Biosensing Techniques , CHO Cells , Cell Line , Chickens , Cricetinae , Dimerization , Epidermal Growth Factor/antagonists & inhibitors , ErbB Receptors/biosynthesis , ErbB Receptors/isolation & purification , Growth Inhibitors/genetics , Growth Inhibitors/metabolism , Humans , Ligands , Mice , Mutagenesis, Site-Directed , Peptide Fragments/biosynthesis , Peptide Fragments/isolation & purification , Plasmids/biosynthesis , Plasmids/metabolism , Protein Binding/genetics , Protein Structure, Tertiary/genetics , Transfection , Transforming Growth Factor alpha/metabolism
14.
Mol Pathol ; 54(3): 125-32, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11376122

ABSTRACT

Ever since the discovery of insulin and its role in the regulation of glucose metabolism, there has been great interest in the molecule itself, the insulin-like growth factors (IGFs), and their receptors (IR and IGF-R). These receptors form a subfamily of tyrosine kinase receptors which are large, transmembrane proteins consisting of several structural domains. Their ectodomains have a similar arrangement of two homologous domains (L1 and L2) separated by a Cys rich region. The C-terminal half of their ectodomains consists of three fibronectin type 3 repeats, and an insert domain that contains the alpha-beta cleavage site. This review summarises the key developments in the understanding of the structure of this family of receptors and their relation to other multidomain proteins. Data presented will include multiple sequence analyses, single molecule electron microscope images of the IGF-1R, insulin receptor (IR), and IR-Fab complexes, and the three dimensional structure of the first three domains of the IGF-1R determined to 2.6 A resolution by x ray crystallography. The L domains each adopt a compact shape consisting of a single stranded, right handed beta-helix. The Cys rich region is composed of eight disulphide bonded modules, seven of which form a rod shaped domain with modules associated in an unusual manner.


Subject(s)
Receptor, IGF Type 1/chemistry , Crystallography, X-Ray , Dimerization , Disulfides/chemistry , Humans , Ligands , Microscopy, Electron , Receptor, Insulin/chemistry , Sequence Analysis, Protein
15.
Liver ; 21(2): 149-58, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11318985

ABSTRACT

BACKGROUND/AIMS: Growth hormone (GH), when overexpressed in male and female GH-transgenic mice, is known to induce liver tumours within 1 year. This study aimed to gain a clearer understanding of the interaction between GH and tumour cells in vivo. METHODS/RESULTS: The carcinogen diethylnitrosomine (DEN) was administered to neo-natal transgenic and non-transgenic mice maintained in a "hepatocarcinogenesis resistant" genetic background (C57BL/6J). Macroscopic, microscopic and liver weight/body weight ratio analyses revealed that carcinogen-induced hepatocarcinogenesis was dramatically accelerated in young GH-transgenic mice compared to non-transgenic counterparts. Image analysis of microscopic hepatocellular neoplasms showed rapidly increasing tumour burdens, and neoplastic foci size over time in young adult GH-transgenic mice. The magnitude of enhanced tumour growth was equivalent in both male and female transgenic mice, whereas much lower and sexually dimorphic tumour growth rates (males>females) were observed in non-transgenic mice treated with DEN. BrdU labelling experiments demonstrated that rapid tumour growth in carcinogen-treated GH-transgenic mice was due to the promotion of cell proliferation in emerging lesions. Tumour cell proliferation in young GH-transgenic mice was 2.6- and 4-fold higher, respectively, than that observed in similar age male and female non-transgenic mice. Interestingly, both GH-transgenic and non-transgenic mice displayed progressively slower tumour growth rates in older animals. CONCLUSION: Overall, GH synergistically promotes carcinogen-induced hepatocarcinogenesis in both sexes of GH-transgenic mice by stimulating tumour cell proliferation.


Subject(s)
Focal Nodular Hyperplasia/metabolism , Growth Hormone/biosynthesis , Liver Neoplasms, Experimental/metabolism , Precancerous Conditions/metabolism , Animals , Animals, Newborn , Apoptosis , Bromodeoxyuridine/analysis , Bromodeoxyuridine/metabolism , Cell Division/drug effects , Diethylnitrosamine/toxicity , Female , Focal Nodular Hyperplasia/chemically induced , Focal Nodular Hyperplasia/genetics , Focal Nodular Hyperplasia/pathology , Growth Hormone/genetics , Image Processing, Computer-Assisted , Immunoenzyme Techniques , In Situ Nick-End Labeling , Liver/chemistry , Liver/drug effects , Liver/pathology , Liver Neoplasms, Experimental/chemically induced , Liver Neoplasms, Experimental/genetics , Liver Neoplasms, Experimental/pathology , Male , Mice , Mice, Inbred C57BL , Mice, Transgenic , Organ Size/drug effects , Precancerous Conditions/chemically induced , Precancerous Conditions/genetics , Precancerous Conditions/pathology , Time Factors
16.
J Anim Sci ; 78(9): 2237-43, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10985393

ABSTRACT

The effect of active immunization against GnRH on production, carcass, and behavioral traits was examined in ram lambs fed to a uniform slaughter weight. Lambs (initial BW = 32.6+/-1 kg) were stratified by BW and assigned at random to one of four treatment groups (n = 12 lambs/group). Lambs were untreated, castrated, or actively immunized against GnRH using a GnRH-keyhole limpet hemocyanin conjugate (1 mg) emulsified with either Freund's complete adjuvant (FCA) or another oil-based adjuvant (ISA). Animals were housed individually and slaughtered at 58 kg BW. Immunoneutralization of GnRH reduced (P < .05) testes weight and the concentration of testosterone in serum at slaughter. Suppression of testicular size and function was most clearly evident in animals immunized using FCA. Final anti-GnRH titer was also highest in lambs immunized using FCA. Several measures of sexual behavior (frequency of mounts and ejaculations) were also reduced (P < .05) in animals immunized using FCA. The duration of the feeding period was greater (P < .05) for castrated lambs than for untreated lambs, and intermediate feeding periods were required for FCA and ISA lambs. Average daily gain was greater (P < .05) in untreated than in castrated, FCA, or ISA lambs. Similarly, feed efficiency for untreated lambs was greater (P < .05) than for castrated, FCA, or ISA lambs, but feed efficiency did not differ among castrated, FCA, or ISA lambs. Longissimus muscle area, lean and bone maturity, overall quality, muscling score, flank streaking, and color of fat did not differ among treatments. Intact, FCA, and ISA lambs had more (P < .05) desirable yield grades, less (P < .05) backfat, and less (P < .05) marbling than castrated lambs. In summary, immunization against GnRH decreased testicular weight and reduced (P < .05) feedlot performance and sexual behavior to levels comparable to those of castrated males. Partitioning of nutrients for growth and deposition of fat, however, seems to differ among immunologically castrated and physically castrated lambs. This difference in nutrient partitioning may be due to residual testicular activity in immunized lambs.


Subject(s)
Gonadotropin-Releasing Hormone/immunology , Sexual Behavior, Animal/physiology , Sheep/growth & development , Testis/physiology , Vaccination/veterinary , Animal Feed , Animal Husbandry , Animals , Body Weight , Male , Testis/growth & development
17.
Anim Reprod Sci ; 63(3-4): 167-75, 2000 Nov 01.
Article in English | MEDLINE | ID: mdl-10989227

ABSTRACT

Stress-like levels of cortisol suppress follicular growth and development and block or delay the preovulatory surge of LH when cortisol is continuously administered during the late luteal and early follicular phases of the ovine oestrous cycle. We postulated that cortisol infusion of shorter duration would have a similar effect. To test this hypothesis the oestrous cycles of mature ewes were synchronized using progestin-treated vaginal pessaries. Ewes were randomly assigned to one of four treatment groups. Animals received cortisol (0.1mg/kg/h; n=8) or vehicle alone (n=8) beginning 5 days before, and continuing for 5 days after, pessary removal (PR). Additional groups received cortisol only during the 5 days period before (n=7), or the 5 days period after (n=8), PR. Continuous delivery of cortisol established stable serum concentrations of cortisol of 72.0+/-2.5ng/ml within 6h of initiation of infusion. Serum concentrations of oestradiol increased progressively during the period after PR in control animals receiving vehicle alone and the preovulatory surge of LH was evident in all control animals (eight of eight) 55.5+/-5.0h after PR. In contrast, follicular development and the preovulatory surge of LH were evident during the period of cortisol infusion in only one of eight animals receiving stress-like levels of cortisol over the entire 10-day infusion period. Similarly, neither follicular development nor surge-like secretion of LH were evident during the infusion period in animals (zero of eight) receiving cortisol during the 5-day period after PR. This cortisol-dependent suppression of ovarian activity in sheep receiving stress-like levels of cortisol during the 5 days after PR was temporary and follicular development, the ovulatory surge of LH, and subsequent luteal function were evident in six of eight ewes after cessation of cortisol delivery. Similarly, follicular development and the preovulatory surge of LH were noted within 5 days after PR in four of seven ewes receiving cortisol only during the 5-day period prior to PR. Collectively, these data indicate that stress-like levels of cortisol reduce fertility of sheep by suppressing follicular development and the preovulatory surge of LH. Additionally, cortisol delivery during the follicular phase has a more profound suppressive effect on follicular development than cortisol administration during the luteal phase.


Subject(s)
Hydrocortisone , Luteinizing Hormone/blood , Ovarian Follicle/growth & development , Sheep Diseases/physiopathology , Stress, Physiological/veterinary , Animals , Female , Ovarian Follicle/drug effects , Ovulation/drug effects , Progesterone/blood , Pulsatile Flow , Random Allocation , Sheep , Stress, Physiological/physiopathology
18.
Cell Mol Life Sci ; 57(7): 1050-93, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10961344

ABSTRACT

The type 1 insulin-like growth factor receptor (IGF-1R), a transmembrane tyrosine kinase, is widely expressed across many cell types in foetal and postnatal tissues. Activation of the receptor following binding of the secreted growth factor ligands IGF-1 and IGF-2 elicits a repertoire of cellular responses including proliferation, and the protection of cells from programmed cell death or apoptosis. As a result, signalling through the IGF-1R is the principal pathway responsible for somatic growth in foetal mammals, whereas somatic growth in postnatal animals is achieved through the synergistic interaction of growth hormone and the IGFs. Forced overexpression of the IGF-1R results in the malignant transformation of cultured cells: conversely, downregulation of IGF-1R levels can reverse the transformed phenotype of tumour cells, and may render them sensitive to apoptosis in vivo. Elevated levels of IGF-IR are observed in a variety of human tumour types, whereas epidemiological studies implicate the IGF-1 axis as a predisposing factor in the pathogenesis of human breast and prostate cancer. The IGF-1R has thus emerged as a therapeutic target for the development of antitumour agents. Recent progress towards the elucidation of the three-dimensional structure of the extracellular domain of the IGF-1R represents an opportunity for the rational assembly of small molecule antagonists of receptor function for clinical use.


Subject(s)
Receptor, IGF Type 1/chemistry , Receptor, IGF Type 1/metabolism , Amino Acid Sequence , Animals , Antibodies, Monoclonal/immunology , Apoptosis , Cell Transformation, Neoplastic , Evolution, Molecular , Humans , Insulin/chemistry , Insulin/genetics , Insulin/metabolism , Molecular Sequence Data , Neoplasms/therapy , Protein Conformation , Receptor, IGF Type 1/genetics , Receptor, IGF Type 1/immunology , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Signal Transduction
19.
Anim Reprod Sci ; 59(3-4): 167-78, 2000 May 31.
Article in English | MEDLINE | ID: mdl-10837977

ABSTRACT

The effect of stress-like concentrations of cortisol on oestradiol-induced change in LH secretion and GnRH receptor expression was evaluated in orchidectomized sheep (wethers). Twenty-four wethers were assigned at random to one of the four treatment groups in a 2x2 factorial design (n=6 wethers/group). Wethers received cortisol (90 microg/kg/h; groups 2 and 4) or a comparable volume of cortisol delivery vehicle (groups 1 and 3) by continuous infusion for 48 h. During the final 24 h of infusion, wethers received oestradiol (6 ng/kg/h; groups 3 and 4) or oestradiol delivery vehicle (groups 1 and 2). The pattern of LH secretion was assessed during a 3-h period of intensive blood collection beginning 21 h after initiation of oestradiol infusion. Although neither cortisol nor oestradiol alone affected (P>0.05) mean serum concentration of LH or LH pulse frequency, serum LH and the frequency of secretory episodes of LH were significantly reduced (P<0.05) in wethers receiving cortisol and oestradiol in combination. Anterior pituitary tissue was collected at the end of the infusion period. Oestradiol increased (P<0.05) tissue concentrations of GnRH receptor and GnRH receptor mRNA. Although cortisol alone did not affect (P>0.05) basal concentrations of receptor or receptor mRNA, the magnitude of oestradiol-induced increase in GnRH receptor and GnRH receptor mRNA was significantly reduced in wethers receiving cortisol and oestradiol concurrently. Conversely, steady-state concentrations of mRNA encoding the LHbeta and FSHbeta subunits were increased (P<0.05) in wethers receiving cortisol. These observations demonstrate that stress-like concentrations of cortisol act in concert with oestradiol to suppress LH secretion. In addition, cortisol blocks oestradiol-dependent increase in pituitary tissue concentrations of GnRH receptor and GnRH receptor mRNA.


Subject(s)
Estradiol/pharmacology , Hydrocortisone/blood , Orchiectomy , Sheep/blood , Stress, Physiological , Animals , Estradiol/blood , Feedback , Follicle Stimulating Hormone/blood , Follicle Stimulating Hormone/metabolism , Hydrocortisone/pharmacology , Luteinizing Hormone/blood , Luteinizing Hormone/metabolism , Male , Pituitary Gland, Anterior/drug effects , Pituitary Gland, Anterior/metabolism , RNA, Messenger/metabolism , Receptors, LHRH/genetics , Receptors, LHRH/metabolism
20.
FEBS Lett ; 469(1): 57-60, 2000 Mar 03.
Article in English | MEDLINE | ID: mdl-10708756

ABSTRACT

The insulin receptor (IR) and the insulin-like growth factor-I receptor (IGF-1R) show differential binding of insulin and IGFs. The specificity determinants for IGF-1 binding are known to be located in the cysteine-rich (Cys-rich) region between residues 223 and 274 of human IGF-1R, which includes a loop that protrudes into the putative ligand binding site. In this report we have replaced residues 260-277 of human IR with residues 253-266 of the human IGF-1R to produce an IR-based, cysteine loop exchange chimaera, termed hIR-Cys loop exchange (CLX), in which all 14 amino acid residues in the exchanged loop differ from wild-type insulin receptor. This loop exchange had a detrimental effect on the efficiency of pro-receptor processing and on the binding of the mouse monoclonal antibody 83-7. However, this antibody, which binds hIR but not hIGF-1R, was still capable of immunoprecipitating the mature chimaeric receptor, indicating that the conformational epitope recognised by this antibody is not primarily determined by the loop region exchanged. The loop exchange did not significantly affect the ability of insulin to displace bound radiolabelled insulin, but increased the capacity of IGF-1 to competitively displace labelled insulin by at least 10 fold.


Subject(s)
Receptor, IGF Type 1/genetics , Receptor, Insulin/genetics , Recombinant Fusion Proteins/metabolism , Amino Acid Sequence , Antibodies, Monoclonal/immunology , Binding, Competitive , Epitopes , Humans , Insulin/metabolism , Iodine Radioisotopes , Molecular Sequence Data , Receptor, IGF Type 1/metabolism , Receptor, Insulin/metabolism , Recombinant Fusion Proteins/immunology
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