ABSTRACT
The African Catfish (Clarias gariepinus) are important source of protein for local consumers in developing countries in Africa and have also been reported to have enormous commercial potential. Several works have been done on plethora of general histological, biochemical and hormone changes which accompany puberty in African Catfish. Other studies have touched the effects of ecotoxins on the histological and reproductive parameters of the mature African Catfish. This study is an attempt to use immunohistochemical and basic histology to elucidate the baseline information on the general structural differences between the testes of immature and post-pubertal catfish with respect to some intermediate filaments arrangement within the testicular tissue. Ten (10) each of mature male catfish (4-5 months old) and immature male catfish (3 months old) were used in the study. The fish were subjected to cold shock and decapitated before the testes were harvested from both groups. These tissues were fixed in Bouins fluid for 24 hours and subsequently transferred into 70% Ethanol. Testicular tissues from both groups were processed for paraffin embedding for routine staining. Another set of tissues were fixed in Neutral Buffered Formalin for testicular immunostaining techniques for expression of Vimentin, Desmin, Cytokeratin and Smooth Muscle Actin. There is an increase in seminiferous luminal area in the mature catfish testis with the presence of mature spermatozoa in the lumen when compared with immature catfish testis which has small size of lumen with absence of mature spermatozoa. Testicular interstitium thickness remain relatively unchanged. SMA was markedly expressed in the cytoplasm of interstitial Leydig cells in the immature catfish testis whereas it was weak in its expression in the mature catfish. However, SMA was not expressed in the connective tissue proper in the testicular interstitium. Cytokeratin expression was also marked in the testicular capsule of immature catfish but was weak to absent in the mature catfish, however, both mature and immature catfish had moderate cytokeratin expression in their seminiferous tubule basement membrane. Desmin was strongly expressed in cytoplasm of immature germinal cells in the immature catfish testis but was moderate in its expression in the mature catfish testis. Vimentin expression was marked in the cytoplasm of immature germinal cells in both immature and mature catfish testis but weak in its expression in the Sertoli cell cytoplasm of both groups. This study infers that ultra-structural and protein changes can be related to age changes alone apart from the contribution of seasonality and external interference by ecotoxins. The age-related changes seen in this study could set ``baseline information. The extent of contribution of season and other external factors will be better understood. Though the age-related difference might be peculiar to the species of current interest, the differences elucidated are a sound background for relational studies, especially on the effect of ecologic toxins on immature testis, as separate from the mature testis.
Subject(s)
Aging/pathology , Catfishes/anatomy & histology , Intermediate Filaments/ultrastructure , Testis/anatomy & histology , Animals , MaleABSTRACT
Radiography is a practical screening tool to detect subtle skeletal abnormalities in the adult catfish. Systematic identification of skeletal abnormalities in vertebrates provides an insight into the pathogenesis of skeletal disorders. In the present study; we conducted a mutagenesis screen for skeletal dysplasias in adult catfish, using photography and radiography to assess abnormalities in skeletal anatomy and bone morphology of the head. Two of the fish had skeletal protuberances on the head, while the third had a skeletal depression on the head with associated jaw deformity. However, there was no spinal involvement or other skeletal abnormalities. This study suggests a need for closer monitoring of cultured fish species; including dietary and nutritional requirements, breeding and exposure to pollution and pollutants.
La radiografía es una herramienta práctica de la investigación que fue utilizada para detectar sutiles alteraciones esqueléticas en el pez gato adulto. La identificación sistemática de las anomalías esqueléticas de los vertebrados da una idea de la patogénesis de los trastornos esqueléticos. En el presente estudio, se realizó una pantalla de mutagénesis de displasias esqueléticas en el pez gato adulto, utilizando la fotografía y la radiografía para evaluar las anomalías en la anatomía del esqueleto y la morfología ósea de la cabeza. Dos de los peces tenían protuberancias óseas en la cabeza, mientras que el tercero tenía una depresión ósea en la cabeza asociada a deformidades de la mandíbula. Sin embargo, no hubo participación de la columna vertebral u otras anomalías esqueléticas. Este estudio sugiere la necesidad de una vigilancia más estrecha de las especies de peces cultivados, incluyendo dietética, nutrición, reproducción y exposición a la contaminación y los contaminantes.
Subject(s)
Animals , Catfishes , Bone and Bones/abnormalities , Bone and Bones , Skull/abnormalities , Skull , Photography , RadiographyABSTRACT
The correlation between chest girth and weight of sheep and goats have been studied as a predictive guide in homestead farms where equipment to definitively quantify the weight of animals are unavailable and inaccessible. The livestock markets in the South Western part of Nigeria are still very underdeveloped and consist of peasant farmers taking their few animals to more developed areas to offer them for better prices to the consumer or middle men who are in the trade to attain the best profit. Twenty each of sheep and goats were randomly selected in herds from three different commercial locations and evaluated using weight and chest girth measurements as indices. Mean weight for the 3 groups of sheep was between 19.8 and 31.15kg, while for the 3 groups of goats was between 19.6 and 16.35kg. There was correlation (0.39-0.80) between sheep chest girth and weight which does not differ from those already established in literature and apredictive index of W= (18.9 +/- 0.36) CG where in more than 80 percent of the population are accounted for and in goats, correlation of 0.40-0.76 and a predictive index of W= (18.51 +/-0.31) CG where more than 76 percent of the population falls in. It was therefore concluded that chest girth is a useful tool in predicting weight of commercial sheep and goat.
La correlación entre la circunferencia de pecho y el peso de las ovejas y cabras ha sido estudiada como una guía de predicción en granjas, donde el equipo para cuantificar el peso de los animales no está disponible o es de difícil acceso. Los mercados de ganado en la parte sur-occidental de Nigeria, siguen siendo aún muy subdesarroliados y constan de campesinos que tienen pocos animales, a diferencia de zonas más desarrolladas, para ofrecer los mejores precios para el consumidor quienes están los comercializan para alcanzar el mejor beneficio. Veinte ovejas y veinte cabras fueron seleccionadas al azar entre los rebaños de tres diferentes localidades comerciales, y fueron evaluadas utilizando el peso y la circunferencia de pecho como los índices de mediciones. La media de peso de los 3 grupos de ovejas fue entre 19,8 y 31,15kg, respectivamente, mientras que para los 3 grupos de cabras fue entre 19,6 y 16,35kg. Hubo correlación (0,39-0,80) entre la circunferencia de pecho de las ovejas y el peso, que no difieren de las ya establecidas en la bibliografía y un índice predictivo de W = (18,9 +/- 0,36) CG en donde más del 80 por ciento de la población se contabilizan y en el ganado caprino, la correlación de 0,40-0,76 y un índice predictivo deW = (18,51 +/- 0,31)CG donde se encuentra más del 76 por ciento de la población. Por lo tanto, se llegó ala conclusión que la circunferencia de pecho es una herramienta útil en la predicción del peso vivo de las ovejas y cabras comerciales.
