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1.
J Environ Manage ; 212: 424-432, 2018 Apr 15.
Article in English | MEDLINE | ID: mdl-29455150

ABSTRACT

Fluoride has both detrimental and beneficial effects on living beings depending on the concentration and consumption periods. The study presented in this article investigated the feasibility of using neem oil phenolic resin treated lignocellulosic bio-sorbents for fluoride removal from water through fixed bed column study. Results indicated that treated bio-sorbents could remove fluoride both from synthetic and groundwater with variable bed depth, flow rate, fluoride concentration and column diameter. Data obtained from this study indicated that columns with the thickest bed, lowest flow rate, and fluoride concentration showed best column performance. Bio-sorbents used in this study are regenerable and reusable for more than five cycles. The initial materials cost needed to remove one gram of fluoride also found to be lower than the available alternatives. This makes the process more promising candidate to be used for fluoride removal. In addition, the process is also technically advantageous over the available alternatives.


Subject(s)
Formaldehyde , Glycerides , Phenols , Polymers , Terpenes , Water Purification , Adsorption , Fluorides , Water , Water Pollutants, Chemical
2.
ACS Appl Mater Interfaces ; 8(24): 14986-93, 2016 Jun 22.
Article in English | MEDLINE | ID: mdl-27266368

ABSTRACT

Plasmonic characteristics of graphene-silver (GAg) nanocomposite coupled with piezoelectric property of Poly(vinylidene fluoride) (PVDF) have been utilized to realize a new class of self-powered flexible plasmonic nanogenerator (PNG). A few layer graphene has been prepared in a facile and cost-effective method and GAg doped PVDF hybrid nanocomposite (PVGAg) is synthesized in a one-pot method. The PNG exhibits superior piezoelectric energy conversion efficiency (∼15%) under the dark condition. The plasmonic behavior of GAg nanocomposite makes the PNG highly responsive to the visible light illumination that leads to ∼50% change in piezo-voltage and ∼70% change in piezo-current, leading to enhanced energy conversion efficiency up to ∼46.6%. The piezoelectric throughput of PNG (e.g., capacitor charging performance) has been monitored during the detection of the different wavelengths of visible light illumination and showed maximum selectivity to the green light. The simultaneous mechanical energy harvesting and visible-light detection capabilities of the PNG are attractive for futuristic self-powered optoelectronic smart sensors and devices.

3.
Indian J Microbiol ; 55(3): 258-68, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26063935

ABSTRACT

ABSTRACT: In the present study, artificial neural network (ANN) modelling coupled with particle swarm optimization (PSO) algorithm was used to optimize the process variables for enhanced low density polyethylene (LDPE) degradation by Curvularia lunata SG1. In the non-linear ANN model, temperature, pH, contact time and agitation were used as input variables and polyethylene bio-degradation as the output variable. Further, on application of PSO to the ANN model, the optimum values of the process parameters were as follows: pH = 7.6, temperature = 37.97 °C, agitation rate = 190.48 rpm and incubation time = 261.95 days. A comparison between the model results and experimental data gave a high correlation coefficient ([Formula: see text]). Significant enhancement of LDPE bio-degradation using C. lunata SG1by about 48 % was achieved under optimum conditions. Thus, the novelty of the work lies in the application of combination of ANN-PSO as optimization strategy to enhance the bio-degradation of LDPE.

4.
3 Biotech ; 5(4): 455-463, 2015 Aug.
Article in English | MEDLINE | ID: mdl-28324548

ABSTRACT

Lactic acid, a biologically derived compound, exists ubiquitously in nature. Its existence ranges from human being to microorganisms. Having paramount industrial significance, lactic acid should be highly pure, devoid of any contaminants. Hence, development of minimum steps of platform technologies to purify it needs urgent attention. The article proposed a novel and simple process for separation of lactic acid from a potential substrate Zizyphus oenophlia, based on ion exchange chromatography. The process herein involves two steps of purification; firstly a weak anion exchange resin was used to separate lactic acid from other anions present in the broth. This was followed by use of strong cation exchanger which washes out the target molecule (lactic acid) while trapped other cations present in the solution. The selected ion exchangers were Amberlite IRA 96 and Amberlite IR 120. Amberlite IRA 96 retained the lactic acid from the broth while washing away other anions. Maximum binding capacity of the resin was found to 210.46 mg lactic acid/g bead. After the simple two-step purification process, the purity of lactic acid improves up to 99.17 % with a recovery yield of 98.9 %. Upon characterization, formation of only levo rotatory form of lactic acid confirms its easy metabolism by the human system, thus triggering its application towards biomaterial sector.

5.
Mol Carcinog ; 54(12): 1636-55, 2015 Dec.
Article in English | MEDLINE | ID: mdl-25358602

ABSTRACT

Reactive oxygen species (ROS), the key mediators of cellular oxidative stress and redox dysregulation involved in cancer initiation and progression, have recently emerged as promising targets for anticancer drug discovery. Continuous free radical assault upsets homeostasis in cellular redox system and regulates the associated signaling pathways to mediate stress-induced cell death. This study investigates the dose-specific pro-oxidative behavior of a bacterial fucose polysaccharide, which attenuated proliferation of different cancer cells. In the fermentation process, Bacillus megaterium RB-05 [GenBank Accession Number HM371417] was found to biosynthesize a polysaccharide with low-fucose content (4.9%), which conferred the maximum anti-proliferative activity (750 µg/mL) against human lung cancer epithelial cells (A549) during preliminary screening. Structural elucidation and morphological characterization of the duly purified polysaccharide was done using HPLC, GC-MS, (1)H/(13)C NMR, and microscopy. The polysaccharide exhibited concentration- and time-dependent anti-proliferative effects against A549 cells by inducing intracellular ROS level and regulating the mitochondrial membrane-permeability following the apoptotic pathway. This process encompasses activation of caspase-8/9/3/7, increase in the ratio of Bax/Bcl2 ratio, translocation of Bcl2-associated X protein (Bax) and cytochrome c, decrease in expression of anti-apoptotic members of Bcl2 family, and phosphorylation of mitogen activated protein kinases (MAPKs). Apoptosis was attenuated upon pretreatment with specific caspase-inhibitors. Simultaneously, during apoptosis, the ROS-mediated stress as well as activated MAPKs triggered nuclear translocation of transcription factors like nuclear factor (erythroid-derived)-like 2 (Nrf2) and promoted further transcription of downstream cytoprotective genes, which somehow perturbed the chemotherapeutic efficacy of the polysaccharide, although using CuPP, a chemical inhibitor of HO-1, apoptosis increased significantly (P < 0.05).


Subject(s)
Apoptosis/drug effects , Intracellular Signaling Peptides and Proteins/metabolism , Lung Neoplasms/metabolism , Mitochondria/drug effects , Mitogen-Activated Protein Kinase Kinases/metabolism , NF-E2-Related Factor 2/metabolism , Polysaccharides, Bacterial/pharmacology , Apoptosis Regulatory Proteins/metabolism , Carcinoma/metabolism , Cell Line, Tumor , Epithelial Cells/metabolism , Fucose/pharmacology , HeLa Cells , Homeostasis/drug effects , Humans , Kelch-Like ECH-Associated Protein 1 , MCF-7 Cells , Mitochondria/metabolism , Reactive Oxygen Species/metabolism , Signal Transduction/drug effects
6.
PLoS One ; 9(11): e113663, 2014.
Article in English | MEDLINE | ID: mdl-25412177

ABSTRACT

Continuous free radical assault upsets cellular homeostasis and dysregulates associated signaling pathways to promote stress-induced cell death. In spite of the continuous development and implementation of effective therapeutic strategies, limitations in treatments for stress-induced toxicities remain. The purpose of the present study was to determine the potential therapeutic efficacy of bacterial fucose polysaccharides against hydrogen peroxide (H2O2)-induced stress in human lung fibroblast (WI38) cells and to understand the associated molecular mechanisms. In two different fermentation processes, Bacillus megaterium RB-05 biosynthesized two non-identical fucose polysaccharides; of these, the polysaccharide having a high-fucose content (∼ 42%) conferred the maximum free radical scavenging efficiency in vitro. Structural characterizations of the purified polysaccharides were performed using HPLC, GC-MS, and (1)H/(13)C/2D-COSY NMR. H2O2 (300 µM) insult to WI38 cells showed anti-proliferative effects by inducing intracellular reactive oxygen species (ROS) and by disrupting mitochondrial membrane permeability, followed by apoptosis. The polysaccharide (250 µg/mL) attenuated the cell death process by directly scavenging intracellular ROS rather than activating endogenous antioxidant enzymes. This process encompasses inhibition of caspase-9/3/7, a decrease in the ratio of Bax/Bcl2, relocalization of translocated Bax and cytochrome c, upregulation of anti-apoptotic members of the Bcl2 family and a decrease in the phosphorylation of MAPKs (mitogen activated protein kinases). Furthermore, cellular homeostasis was re-established via stabilization of MAPK-mediated Nrf2/Keap1 signaling and transcription of downstream cytoprotective genes. This molecular study uniquely introduces a fucose-rich bacterial polysaccharide as a potential inhibitor of H2O2-induced stress and toxicities.


Subject(s)
Apoptosis/drug effects , Free Radical Scavengers/pharmacology , Hydrogen Peroxide/toxicity , Polysaccharides, Bacterial/pharmacology , Reactive Oxygen Species/metabolism , Signal Transduction/drug effects , Bacillus megaterium/metabolism , Carbohydrate Sequence , Caspases/metabolism , Cell Line , Cell Membrane Permeability/drug effects , Cell Proliferation/drug effects , Cytochromes c/metabolism , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/metabolism , Free Radical Scavengers/analysis , Free Radical Scavengers/chemistry , Fucose/chemistry , Fucose/metabolism , Humans , Intracellular Signaling Peptides and Proteins/metabolism , Kelch-Like ECH-Associated Protein 1 , Lung/cytology , Mitochondria/metabolism , Mitogen-Activated Protein Kinases/metabolism , Molecular Sequence Data , NF-E2-Related Factor 2/metabolism , Polysaccharides, Bacterial/analysis , Polysaccharides, Bacterial/chemistry
7.
Colloids Surf B Biointerfaces ; 116: 169-75, 2014 Apr 01.
Article in English | MEDLINE | ID: mdl-24463149

ABSTRACT

The present work focuses on the design of a drug delivery system for systemic, controlled release of the poorly soluble breast cancer drug, letrozole. The drug delivery system was prepared in two steps: a low density polyethylene (LDPE) substrate surface was grafted with maleic anhydride (MA) via solution grafting technique. Next, the grafted substrate was used to anchor a hydrophilic polymeric drug release system consisting of poly (vinyl alcohol) (PVA). The PVA anchored MA grafted LDPE (PVA/MA-g-LDPE) drug release system was used for the controlled release of letrozole. This system was characterized using ATR-FTIR spectrophotometry, surface profilometry, and scanning electron microscopy. Biocompatibility studies were also carried out. In vitro release studies of letrozole from the system were performed in distilled water and phosphate buffer saline (PBS) at 37°C. Release of ∼90% letrozole from hydrophilic PVA matrix was observed within a period of 35 days. A high correlation coefficient (R(2)=0.99) was seen between the release of letrozole in distilled water and PBS. Cytotoxicity studies using MTT colorimetric assay suggested that all samples were biocompatible. It is concluded that the letrozole delivery system appears to overcome the limitations associated with letrozole by providing enhanced drug dissolution rate, controlled release and improved bioavailability of the incorporated drug and, therefore, seems to have extended therapeutic effects.


Subject(s)
Breast Neoplasms/drug therapy , Drug Delivery Systems , Maleic Anhydrides/chemistry , Nitriles/pharmacology , Polyethylene/chemistry , Polyvinyl Alcohol/chemistry , Triazoles/pharmacology , Animals , Breast Neoplasms/pathology , Cell Adhesion/drug effects , Cell Proliferation/drug effects , Cell Survival/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Female , Humans , Letrozole , Mice , Molecular Structure , NIH 3T3 Cells , Nitriles/administration & dosage , Nitriles/chemistry , Structure-Activity Relationship , Triazoles/administration & dosage , Triazoles/chemistry
8.
Bioresour Technol ; 133: 627-9, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23465537

ABSTRACT

The objective of the present investigation deals with optimization of L-lactic acid production from Zizyphus oenophlia, a potential low cost substrate. Central composite design (CCD) based on response surface methodology (RSM) was employed as a statistical tool to investigate the effect of substrate: media ratio, temperature, pH and inoculum volume on lactic acid production. Under optimum condition, the experimental yield was 95.09%, which matched well with the predictive yield of 95.45%. HPLC and circular dichroism (CD) analysis of the fermented product was carried out to confirm the presence of lactic acid in its L (+) seriospecificity respectively. For application of lactic acid in biotechnological sector polymerization studies were carried out. The PLA upon recovery through direct polycondensation reaction resulted in the yield of 85%.


Subject(s)
Biotechnology/methods , Lactic Acid/biosynthesis , Ziziphus/metabolism , Analysis of Variance , Catalysis , Chromatography, High Pressure Liquid , Fruit/metabolism , Lactic Acid/chemistry , Lactobacillus/metabolism , Molecular Weight , Polyesters , Polymers , Stereoisomerism , Viscosity
9.
Carbohydr Polym ; 93(2): 597-603, 2013 Apr 02.
Article in English | MEDLINE | ID: mdl-23499101

ABSTRACT

A bio-catalyzed process has been developed for treating jute fibers to enhance their tensile strength and resistance against biodegradation. Lipolytic bacteria were used in the process to transesterify jute fibers by replacing hydrophilic hydroxyl groups within cellulose chains with hydrophobic fatty acyl chains. Transesterification of some of the hydroxyl groups within the fiber was confirmed with FTIR, UV-vis spectroscopy, (13)C solid state NMR, gas chromatography and analytical determination of ester content. Biomodified fibers exhibited remarkably smaller affinity to water and moisture and retained 62% of their initial tensile strengths after being exposed to a composting environment over 21 days. The corresponding figure for untreated fibers was only 30%. Efficacy of the process reported herein in terms of tensile strength and biodegradation resistance enhancement of fibers achieved after treatment appears to be comparable with similar chemical processes and better than the enzyme-catalyzed alternatives.


Subject(s)
Malvaceae/chemistry , Tensile Strength , Textile Industry/methods , Bacillus megaterium/enzymology , Bacterial Proteins/chemistry , Biodegradation, Environmental , Cellulase/chemistry , Enzyme Activation , Esterification , Esters/chemistry , Hydrophobic and Hydrophilic Interactions , Lipase/chemistry , Lipolysis , Magnetic Resonance Spectroscopy , Spectroscopy, Fourier Transform Infrared , Water/chemistry
10.
Carbohydr Polym ; 92(2): 2118-27, 2013 Feb 15.
Article in English | MEDLINE | ID: mdl-23399266

ABSTRACT

The present work is an endeavor to prepare lignocellulosic biomass based adsorbent, suitable for removal of organic and inorganic pollutants from industrial effluents. Lignocellulosic Corchorus olitorius fibre (jute fibre) surface was grafted with naturally available polyphenol, tannin, preceded by the epoxy-activation of fibre surface with epichlorohydrin under mild condition in an aqueous suspension. The reaction parameters for the modification, viz., concentration of epichlorohydrin and tannin, time, and temperature were optimized. The successful occurrence of surface modification of jute fibre (JF) was characterized and estimated from weight gain percent, elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction, scanning electron and atomic force microscopy, and thermogravimetric analysis. An extensive analysis of deconvoluted FTIR spectra using the Voigt model was utilized to ensure the surface grafting. The microbiological susceptibility study revealed high persistency of JF towards biodegradation after efficient grafting with tannin.


Subject(s)
Corchorus/chemistry , Green Chemistry Technology , Lignin/chemistry , Tannins/chemistry , Adsorption , Epichlorohydrin/chemistry , Microbiology , Molecular Weight , Surface Properties , Temperature , Time Factors , Vegetables , Water Pollutants, Chemical/chemistry , Water Pollutants, Chemical/isolation & purification
11.
J Biomed Mater Res A ; 101(9): 2526-37, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23359556

ABSTRACT

Tripolyphosphate (TPP) crosslinked chitosan (CH)-based fibrous matrices have potential as bioactive scaffolds for bone tissue engineering. This study describes mechanical, biomineralization, and in vitro bone cell growth and differentiation properties of CH-TPP (chitosan-tripolyphosphate) fibrous scaffolds and compared with that of uncrosslinked CH one. The hydrated CH-TPP scaffolds were viscoelastic in nature and their compressive strength was ~2.9 MPa, which is greater than recent polymer experimental bone scaffolds. This improvement in mechanical properties of CH-TPP scaffold may be beneficial toward cancellous bone graft application. Furthermore, CH-TPP fibers supported in vitro biomineralization with phosphate as nucleation site; however, no significant difference in biomineralization morphology was observed with uncrosslinked CH fibers. Interestingly, a significant improvement in cellular responses (>33% increase in cell number based on DNA quantification) was observed when osteoblast like cells were cultured on the CH-TPP scaffolds than that of CH scaffolds without phosphate group. Enhanced osteoblastic differentiation of MG63 cells on CH-TPP scaffolds was also evidenced. Altogether, the results show that the CH-TPP fibrous scaffolds are encouraging for bone tissue engineering.


Subject(s)
Chitosan/analogs & derivatives , Osteoblasts/cytology , Tissue Scaffolds/chemistry , Alkaline Phosphatase/metabolism , Biocompatible Materials/chemistry , Cell Differentiation , Cell Line , Cell Proliferation , Cell Survival , Chitosan/chemistry , Compressive Strength , Cross-Linking Reagents , Humans , Materials Testing , Microscopy, Electron, Scanning , Osteoblasts/metabolism , Polyphosphates/chemistry , Proteins/metabolism , Tissue Engineering
12.
J Biomed Mater Res A ; 100(4): 1068-79, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22318919

ABSTRACT

Collagen, a major component of native extracellular matrix, has diverse biomedical applications. However, its application is limited due to lack of cost-effective production and risk of disease transmission from bovine sources currently utilized. This study describes fabrication and characterization of nano/micro fibrous scaffolds utilizing collagen extracted from fresh water fish origin. This is the first time collagen extracted from fresh water fish origin was studied for their biocompatibility and immunogenicity. The nano/micro fibrous collagen scaffolds were fabricated through self-assembly owing to its amphiphilic nature and were subsequently cross-linked. In vitro degradation study revealed higher stability of the cross-linked scaffolds with only ~50% reduction of mass in 30 days, while the uncross-linked one degraded completely in 4 days. Further, minimal inflammatory response was observed when collagen solution was injected in mice with or without adjuvant, without significant dilution of sera. The fish collagen scaffolds exhibited considerable cell viability and were comparable with that of bovine collagen. SEM and fluorescence microscopic analysis revealed significant proliferation rate of cells on the scaffolds and within 5 days the cells were fully confluent. These findings indicated that fish collagen scaffolds derived from fresh water origin were highly biocompatible in nature.


Subject(s)
Biocompatible Materials , Collagen/chemistry , Fishes , Animals , Female , Fresh Water , Male , Mice , Mice, Inbred BALB C , Microscopy, Electron, Scanning , Microscopy, Fluorescence , Spectroscopy, Fourier Transform Infrared
13.
J Mater Sci Mater Med ; 23(4): 1085-96, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22311077

ABSTRACT

The fibrous scaffolds are promising for tissue engineering applications because of their close structural resemblance with native extracellular matrix. Additionally, the chemical composition of scaffold is also an important consideration as they have significant influences on modulating cell attachment, morphology and function. In this study, chitosan-tripolyphosphate (TPP) non-woven fibrous scaffolds were prepared through wetspinning process. Interestingly, at physiological pH these scaffolds release phosphate ions, which have significant influences on cellular function. For the first time, cell viability in presence of varying concentration of sodium TPP solution was analyzed and correlated with the phosphate release from the scaffolds during 30 days incubation period. In vitro degradation of the chitosan-TPP scaffolds was higher than chitosan scaffolds, which may be due to decrease in crystallinity as a result of instantaneous ionic cross-linking during fiber formation. The scaffolds with highly interconnected porous structure present a remarkable cytocompatibility for cell growing, and show a great potential for tissue engineering applications.


Subject(s)
Chitosan , Polyphosphates , Tissue Engineering , 3T3 Cells , Animals , Biocompatible Materials , Cell Proliferation , Mice , Microscopy, Electron, Scanning , Microscopy, Fluorescence , Spectroscopy, Fourier Transform Infrared , Thermogravimetry , X-Ray Diffraction
14.
Bioresour Technol ; 107: 222-8, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22209134

ABSTRACT

Chemically modified jute fibres are potentially useful as natural reinforcement in composite materials. Jute fibres were treated with 0.25%-1.0% sodium hydroxide (NaOH) solution for 0.5-48 h. The hydrophilicity, surface morphology, crystallinity index, thermal and mechanical characteristics of untreated and alkali treated fibres were studied.The two-parameter Weibull distribution model was applied to deal with the variation in mechanical properties of the natural fibres. Alkali treatment enhanced the tensile strength and elongation at break by 82% and 45%, respectively but decreased the hydrophilicity by 50.5% and the diameter of the fibres by 37%.


Subject(s)
Alkalies/chemistry , Lignin/chemistry , Models, Statistical , Calorimetry, Differential Scanning , Crystallization , Spectroscopy, Fourier Transform Infrared , Surface Properties , Thermogravimetry , X-Ray Diffraction
15.
J Biomater Sci Polym Ed ; 23(15): 1923-38, 2012.
Article in English | MEDLINE | ID: mdl-21967759

ABSTRACT

Scaffolds comprising a nano- and micro-fibrous architecture are promising for tissue engineering, where nanofibers act as connecting network among microfibers and provide a 3D structural environment and mechanical stability for facilitating cell attachment, proliferation and migration. In this study, a novel structure was developed with polymeric micro and nano combined fibrous architecture, which aims to mimic the native extracellular matrix for tissue regeneration. Chitosan-tripolyphosphate (TPP) microfibers were prepared by wet spinning method, where collagen solution was allowed to self-assemble into nano/microfibers and subsequently freeze-dried for obtaining this combined architecture. To ensure prolonged mechanical stability, the scaffold was cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). This polymeric nano/micro combined scaffold revealed remarkable cellular activity and cytocompatibility towards both fibroblasts and osteoblast like cells and supported improved attachment and proliferation of cells than that of bare chitosan-TPP scaffolds owing to the presence of a bioactive molecule, collagen, in the intermingled form with chitosan-TPP microfiber.


Subject(s)
Chitosan , Collagen , Polyphosphates , Tissue Scaffolds , 3T3 Cells , Animals , Cell Adhesion , Cell Line, Tumor , Cell Proliferation , Cell Survival , Chitosan/chemistry , Collagen/chemistry , Ethyldimethylaminopropyl Carbodiimide/chemistry , Fibroblasts/physiology , Humans , Materials Testing , Mice , Nanofibers/chemistry , Osteoblasts/physiology , Polyphosphates/chemistry , Porosity , Solutions , Succinimides/chemistry , Tissue Engineering/methods , Tissue Scaffolds/chemistry
16.
Carbohydr Res ; 346(16): 2582-8, 2011 Nov 29.
Article in English | MEDLINE | ID: mdl-21962591

ABSTRACT

Incorporation of phosphate groups into a material may be of particular interest as they act as templates for hydroxyapatite growth through complexation with Ca(2+) and thus improve the osteoconduction property. The phosphate groups can be incorporated into chitosan through ionotropic gelation with tripolyphosphate (TPP). Interestingly, the ion pairs formed through negatively charged phosphate groups with protonated amine functionality of chitosan in ionotropic gelation are expected to provide chitosan with an amphoteric character, which may facilitate protein adhesion following enhanced attachment of anchorage dependant cells than chitosan, which shows poor cell adhesion properties. In this study, chitosan-tripolyphosphate (TPP) fibers with varying phosphate contents were prepared through wet spinning in STPP baths of different pH. Gelation kinetics and gel strength of chitosan with STPP solutions of three different pH were evaluated and compared with that of NaOH solution for evaluation of their influence on nature of gelation. The solution pH of STPP baths was found to have significant control on the extent of ionic cross-linking and physico-chemical properties of the fibers. Moreover, this kinetically driven ionotropic gelation of chitosan by TPP results in low degree of crystallinity of chitosan-TPP fibers and consequently their lower thermal stability than chitosan fibers.


Subject(s)
Chitosan/analogs & derivatives , Chitosan/chemistry , Cross-Linking Reagents/chemistry , Polyphosphates/chemistry , Elastic Modulus , Gels , Hydrogen-Ion Concentration , Kinetics , Microscopy, Electron, Scanning , Phosphorylation , Powder Diffraction , Spectrometry, X-Ray Emission , Spectroscopy, Fourier Transform Infrared , Thermogravimetry , Viscosity , X-Ray Diffraction
17.
Bioresour Technol ; 102(11): 6629-32, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21507629

ABSTRACT

Bacillus megaterium RB-05 was grown on glucose and on "tossa-daisee" (Corchorus olitorius)-derived jute, and production and composition of extracellular polysaccharide (EPS) were monitored. An EPS yield of 0.065 ± 0.013 and of 0.297 g ± 0.054 g(-1) substrate after 72 h was obtained for glucose and jute, respectively. EPS production in the presence of jute paralleled bacterial cellulase activity. High performance liquid chromatography (HPLC), matrix assisted LASER desorption/ionization-time of flight (MALDI-ToF) mass spectroscopy, and fourier transform infrared (FT-IR) spectroscopy demonstrated that the EPS synthesized in jute culture (JC) differed from that synthesized in glucose mineral salts medium (GMSM). While fucose was only a minor constituent (4.9 wt.%) of EPS from GMSM, it a major component (41.9 wt.%) of EPS synthesized in JC. This study establishes jute as an effective fermentation substrate for EPS production by a cellulase-producing bacterium.


Subject(s)
Bacillus megaterium/metabolism , Corchorus/metabolism , Extracellular Space/metabolism , Polysaccharides, Bacterial/biosynthesis , Bacillus megaterium/enzymology , Bacillus megaterium/growth & development , Cellulase/metabolism , Monosaccharides/analysis
18.
Int J Biol Macromol ; 48(4): 705-12, 2011 May 01.
Article in English | MEDLINE | ID: mdl-21382404

ABSTRACT

Bacillus pumilus UW-02, an isolate from agricultural soil irrigated with waste water was found to produce a carbohydrate polymer in the form of extracellular polysaccharide (EPS) in glucose mineral salts medium (GMSM). The recovery rates of EPS by ion-exchange and gel filtration chromatography were around 63% and 90%, respectively. As evident from HPLC and FT-IR analyses, the EPS was found to be a heteropolymer consisting glucose, mannose, xylose, arabinose, and N-acetyl glucosamine as monomer units. Different oligosaccharide combinations namely hexose(4), hexose(6) pentose(1) and hexose(10) pentose(1) are obtained after partial hydrolysis of EPS using MALDI-ToF-MS. Electron micrographs portrayed the intense affinity of the EPS molecules for each other, thereby justifying its viscosifying and thickening properties. The EPS with an average molecular weight of 218 kDa and thermal stability up to 180 °C showed pseudoplastic rheology and significant emulsifying activities.


Subject(s)
Bacillus/chemistry , Emulsifying Agents/chemistry , Polysaccharides, Bacterial/chemistry , Soil Microbiology , Chromatography, High Pressure Liquid , Emulsifying Agents/isolation & purification , Microscopy, Electron , Polysaccharides, Bacterial/isolation & purification , Rheology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Spectroscopy, Fourier Transform Infrared , Viscosity
19.
Bioresour Technol ; 101(10): 3737-42, 2010 May.
Article in English | MEDLINE | ID: mdl-20116238

ABSTRACT

Collagen is the most abundant protein found in animal body and widely used for biomedical and pharmaceutical applications. However, its applicability is severely limited due to high cost. Fish processing waste, which otherwise cause serious environmental pollution, is a promising cost effective collagen source. In the present study, collagen was isolated from scales of Labeo rohita (Rohu) and Catla catla (Catla). It is first time that these species are used as sources of collagen. Thermo-gravimetric analysis (TGA) revealed maximum demineralization achieved after 48h of EDTA treatment of intact scale. The isolated protein was confirmed as collagen by different physico-chemical techniques like FTIR, SDS-PAGE, and CD. Further amino acid analysis corroborates isolation of type I collagen. A major characteristic of obtained collagen was found to have denaturation temperature (T(d)) of 36.5 degrees C, which is promising as an advantage for biomedical application due to closeness in T(d) to mammalian collagen.


Subject(s)
Collagen/isolation & purification , Fishes , Animals , Circular Dichroism , Collagen/chemistry , Electrophoresis, Polyacrylamide Gel , Hot Temperature , Spectroscopy, Fourier Transform Infrared
20.
Bioresour Technol ; 101(9): 3182-7, 2010 May.
Article in English | MEDLINE | ID: mdl-20074944

ABSTRACT

The physico-chemical properties of jute fibers treated with alkali (NaOH) solution have been investigated in this study. The treatments were applied under ambient and elevated temperatures and high pressure steaming conditions. To the knowledge of these authors the influence of alkali-steam treatment on the uniaxial tensile strength of natural ligno-cellulosic fibers, such as jute, has not been investigated earlier. The results from this investigation indicate that a 30 min dipping of the fibers in 0.5% alkali solution followed by 30 min alkali-steam treatment leads to an increase in the tensile strength of up to 65%. The increase appears to be due to fiber separation and removal of non-cellulosic materials, which, in turn, resulted in an increased crystallinity.


Subject(s)
Alkalies/pharmacology , Corchorus/chemistry , Corchorus/drug effects , Lignin/chemistry , Materials Testing/methods , Steam , Tensile Strength/drug effects , Crystallization , Materials Testing/economics , Microscopy, Electron, Scanning , Spectroscopy, Fourier Transform Infrared , Surface Properties/drug effects , Temperature , X-Ray Diffraction
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