Changes in environmental and engineered conditions alter the plasma membrane lipidome of fractured shale bacteria.

IMPORTANCE: Microorganisms inadvertently introduced into the shale reservoir during fracturing face multiple stressors including brine-level salinities and starvation. However, some anaerobic halotolerant bacteria adapt and persist for long periods of time. They produce hydrogen sulfide, which sours the reservoir and corrodes engineering infrastructure. In addition, they form biofilms on rock matrices, which decrease shale permeability and clog fracture networks. These reduce well productivity and increase extraction costs. Under stress, microbes remodel their plasma membrane to optimize its roles in protection and mediating cellular processes such as signaling, transport, and energy metabolism. Hence, by observing changes in the membrane lipidome of model shale bacteria, Halanaerobium congolense WG10, and mixed consortia enriched from produced fluids under varying subsurface conditions and growth modes, we provide insight that advances our knowledge of the fractured shale biosystem. We also offer data-driven recommendations for improving biocontrol efficacy and the efficiency of energy recovery from unconventional formations.

Environmental impact of e-waste management in Indian microscale informal sectors.

Inappropriate e-waste processing in the informal sector is a serious issue in developing countries. Field investigations in microscale informal recycling sites have been performed to study the impact of hazardous metal(loid)s (released from e-waste dismantling) on the environment (water and soil). Eight hazardous metal(loid)s (Pb, Cd, Cu, Zn, As, Hg, Ni, and Cr) were primarily found in the monitored water and soil samples (Sangrampur, West Bengal) because of widespread informal e-waste handling and primitive processing. Elevated concentrations of Cd, Pb, As, Cu, and Cr were observed in pond water samples (0.04, 1.62, 0.03, 1.40, 1.74 mg/L respectively). These ponds, which are regularly used for e-waste handling/dismantling, are usually flooded during the monsoon season mixing with further larger water resources - posing a serious threat to public health. Enriched levels of Pb, Cd, Cu, and Zn were detected in collected soil samples, both top surface soil (Pb up to 2042.27 ± 206.80, Cd up to 25.90 ± 9.53, Cu up to 6967.30 ± 711.70, and Zn up to 657.10 ± 67.05 mg/Kg) and deeper subsurface soil (Pb, 419.70 ± 44.70; Cd, 18.34 ± 3.81; Cu, 3928.60 ± 356.40; and Zn, 134.40 ± 33.40 mg/Kg), compared to the levels of As, Hg, Ni, and Cr. Seasonal variation of soil metal(loid) content indicated that higher levels of most of the metal(loids) were detected in the pre-monsoon (Nov-May) season, possibly due to the monsoonal dilution effect, except for Pb and Cd. The results highlighted that the composition and the handling of e-waste were important factors affecting the metal(loid) concentrations. E-waste policy and legislation have great influence on the handling and disposal procedures. An improved e-waste management practice has been proposed to encourage eco-friendly and safe e-waste disposal. It is recommended that regulatory agencies and manufacturers should create a road map to convince the informal sector to develop a systematic approach towards a more standardized formal e-waste management practices at the microscale field level.

Salinity and hydraulic retention time induce membrane phospholipid acyl chain remodeling in Halanaerobium congolense WG10 and mixed cultures from hydraulically fractured shale wells.

Bacteria remodel their plasma membrane lipidome to maintain key biophysical attributes in response to ecological disturbances. For Halanaerobium and other anaerobic halotolerant taxa that persist in hydraulically fractured deep subsurface shale reservoirs, salinity, and hydraulic retention time (HRT) are important perturbants of cell membrane structure, yet their effects remain poorly understood. Membrane-linked activities underlie in situ microbial growth kinetics and physiologies which drive biogeochemical reactions in engineered subsurface systems. Hence, we used gas chromatography-mass spectrometry (GC-MS) to investigate the effects of salinity and HRT on the phospholipid fatty acid composition of H. congolense WG10 and mixed enrichment cultures from hydraulically fractured shale wells. We also coupled acyl chain remodeling to membrane mechanics by measuring bilayer elasticity using atomic force microscopy (AFM). For these experiments, cultures were grown in a chemostat vessel operated in continuous flow mode under strict anoxia and constant stirring. Our findings show that salinity and HRT induce significant changes in membrane fatty acid chemistry of H. congolense WG10 in distinct and complementary ways. Notably, under nonoptimal salt concentrations (7% and 20% NaCl), H. congolense WG10 elevates the portion of polyunsaturated fatty acids (PUFAs) in its membrane, and this results in an apparent increase in fluidity (homeoviscous adaptation principle) and thickness. Double bond index (DBI) and mean chain length (MCL) were used as proxies for membrane fluidity and thickness, respectively. These results provide new insight into our understanding of how environmental and engineered factors might disrupt the physical and biogeochemical equilibria of fractured shale by inducing physiologically relevant changes in the membrane fatty acid chemistry of persistent microbial taxa. GRAPHICAL ABSTRACTSalinity significantly alters membrane bilayer fluidity and thickness in Halanaerobium congolense WG10.

Assessment of toxic metals in groundwater and saliva in an arsenic affected area of West Bengal, India: A pilot scale study.

Communities in many parts of the world are unintentionally exposed to arsenic (As) and other toxic metals through ingestion of local drinking water and foods. The concentrations of individual toxic metals often exceed their guidelines in drinking water but the health risks associated with such multiple-metal exposures have yet to receive much attention. This study examines the co-occurrence of toxic metals in groundwater samples collected from As-rich areas of Nadia district, West Bengal, India. Arsenic in groundwater (range: 12-1064 µg L(-1); mean ± S.D: 329±294 µg L(-1)) was the most important contaminant with concentrations well above the WHO guideline of 10 µg L(-1). Another important toxic metal in the study area was manganese (Mn) with average concentration of 202±153 µg L(-1), range of 18-604 µg L(-1). The average concentrations (µg L(-1)) of other elements in groundwater were: Cr (5.6±5.9), Mo (3.5±2.1), Ni (8.3±8.7), Pb (2.9±1.3), Ba (119±43), Zn (56±40), Se (0.60±0.33), U (0.50±0.74). Saliva collected from the male participants of the area had mean concentrations of 6.3±7.0 µg As L(-1) (0.70-29 µg L(-1)), 5.4±5.5 µg Mn L(-1) (0.69-22 µg L(-1)), 2.6±3.1 µg Ni L(-1) (0.15-13 µg L(-1)), 0.78±1.0µg Cr L(-1) (