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PLoS One ; 8(7): e69544, 2013.
Article in English | MEDLINE | ID: mdl-23894501

ABSTRACT

Nipah virus causes periodic livestock and human disease with high case fatality rate, and consequent major economic, social and psychological impacts. Fruit bats of the genus Pteropus are the natural reservoir. In this study, we used real time PCR to screen the saliva and urine of P. vampyrus from North Sumatera for Nipah virus genome. A conventional reverse transcriptase (RT-PCR) assay was used on provisionally positive samples to corroborate findings. This is the first report of Nipah virus detection in P. vampyrus in Sumatera, Indonesia.


Subject(s)
Chiroptera/virology , Nipah Virus/isolation & purification , Animals , Base Sequence , Chiroptera/blood , Genome, Viral/genetics , Humans , Indonesia , Molecular Sequence Data , Nipah Virus/genetics , Real-Time Polymerase Chain Reaction , Sequence Analysis
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