ABSTRACT
The lysosomal enzymes, non-specific esterases, beta-glucuronidase, N-acetyl-beta-glucosaminidase and aryl sulphatases A and B, were examined histochemically in medial-edge epithelia (MEE) of single palatal shelves in vitro. Activities of most enzymes increased gradually in MEE with a peak at 24-26 h of culture. Aryl sulphatase B activities were lower than the others and aryl sulphatase A activities could not be detected in the palatal cells during the entire culture period. By 48 h, MEE cells degenerated and were lost. Cortisol suppressed increased activities of these hydrolytic enzymes and prevented programmed breakdown of the palatal epithelium.
Subject(s)
Hydrocortisone/pharmacology , Lysosomes/enzymology , Palate/enzymology , Acetylglucosaminidase/metabolism , Animals , Arylsulfatases/metabolism , Carboxylesterase , Carboxylic Ester Hydrolases/metabolism , Epithelium/drug effects , Glucuronidase/metabolism , Mice , Organ Culture Techniques , Palate/drug effects , Palate/embryologyABSTRACT
Several monoclonal antibodies to bovine enamel proteins were produced using the mouse myeloma system. Each antibody recognized the same two protein bands on gel electrophoresis. The clones were tested in situ and clone 185 localized specifically in the enamel layer. Clones 185 and 121 were shown to recognize different antigenic determinants.
