ABSTRACT
BACKGROUND: Skeletal maturation is an integral part of individual pattern of growth and development and is a continuous process. Peak growth velocity in standing height is the most valid representation of the rate of overall skeletal growth. Ossification changes of hand wrist and cervical vertebrae are the reliable indicators of growth status of individual. The objective of this study was to compare skeletal maturation as measured by hand wrist bone analysis and cervical vertebral analysis. MATERIALS AND METHODS: Hand wrist radiographs and lateral cephalograms of 72 subjects aged between 7 and 16 years both male and female from the patients visiting Department of Orthodontics and Dentofacial Orthopedics, R.V. Dental College and Hospital. The 9 stages were reduced to 5 stages to compare with cervical vertebral maturation stage by Baccetti et al. The Bjork, Grave and Brown stages were reduced to six intervals to compare with cervical vertebral maturational index (CVMI) staging by Hassel and Farman. These measurements were then compared with the hand wrist bone analysis, and the results were statistically analyzed using the Mann-Whitney test. RESULTS: There was no significant difference between the hand wrist analysis and the two different cervical vertebral analyses for assessing skeletal maturation. There was no significant difference between the two cervical vertebral analyses, but the CVMI method, which is visual method is less time consuming. CONCLUSION: Vertebral analysis on a lateral cephalogram is as valid as the hand wrist bone analysis with the advantage of reducing the radiation exposure of growing subjects.
ABSTRACT
BACKGROUND: Non Syndromic tooth agenesis is a congenital anomaly with significant medical, psychological and social ramifications. There is sufficient evidence to hypothesize that locus for this condition can be identified by candidate genes. The aim of this study was to test whether MSX1 671 T>C gene variant was involved in etiology of Non Syndromic tooth agenesis in Raichur Patients. MATERIALS & METHODS: Blood samples were collected with informed consent from 50 subjects having Non Syndromic tooth agenesis and 50 controls. Genomic DNA was extracted from the blood samples, Polymerase Chain Reaction was performed (PCR) and Restriction Fragment Length Polymorphism (RFLP) was performed for digestion products that were evaluated. RESULTS: The RESULTS showed positive correlation between MSX1671 T>C gene variant and Non Syndromic tooth agenesis in Raichur Patients. CONCLUSION: MSX1 671 T>C gene variant may be a good screening marker for Non Syndromic tooth agenesis in Raichur Patients . How to cite this article:Reddy NA, Adusumilli G, Devanna R, Pichai S, Rohra MG, Arjunan S. Msx1 Gene Variant - Its Presence in Tooth Absence - A Case Control Genetic Study. J Int Oral Health 2013; 5(5):20-6.
ABSTRACT
INTRODUCTION: Non-syndromic tooth agenesis is a congenital anomaly with significant medical, psychological, and social ramifications. There is sufficient evidence to hypothesize that locus for this condition can be identified by candidate genes. AIM OF THE STUDY: The aim of this study was to test whether MSX1 671 T > C gene variant was involved in etiology of non-syndromic tooth agenesis in Raichur patients. MATERIALS AND METHODS: Blood samples were collected with informed consent from 50 subjects having non-syndromic tooth agenesis and 50 controls. Genomic deoxyribonucleic acid (DNA) was extracted from the blood samples, polymerase chain reaction (PCR) was performed, and restriction fragment length polymorphism (RFLP) was performed for digestion products that were evaluated. RESULTS: The results showed positive correlation between MSX1671 T > C gene variant and non-syndromic tooth agenesis in Raichur patients. CONCLUSION: MSX1 671 T > C gene variant may be a good screening marker for non-syndromic tooth agenesis in Raichur patients.
ABSTRACT
AIM: The aim is to compare and evaluate the airway dimension changes, adenoidal nasopharyngeal ratio (ANR), airway area and airway percentage in patients in pre- and postadenoidectomy with normal individuals. MATERIALS AND METHODS: After obtaining informed consent, a sample of 15 patients (eight males and seven females) of 7 to 12 years were selected for adenoidectomy by an otolaryngologist, lateral cephalograms were taken in natural head position before adenoidectomy and after 1 month postadenoidectomy. Statastical analysis was done to evaluate the results using Statastical Package for Social Sciences. Results showed airway (P1, P2, P3, P4), airway percentage, airway area showed significant increase (p < 0.0001), whereas ANR showed significant reduction after 1 month postadenoidectomy. CONCLUSION: One month postadenoidectomy showed increased airway area, airway percentage and reduced ANR. CLINICAL SIGNIFICANCE: Obstructive mouth breathing due to adenoids in growing children can cause alteration in craniofacial morphology leading to adenoid facies, adenoidectomy procedure helps in alleviating the obstruction and facilitates the normal growth of craniofacial complex.
