ABSTRACT
The problem of retroperitoneal neoplasm management is still relevant despite certain achievements in diagnosis, anesthetic care and surgical techniques. These patients often admit to specialized hospitals with a widespread malignant process and/or complications due to no specific symptoms. Resection is preferred for malignant inorganic retroperitoneal tumors. A feature of these neoplasms is frequent local relapses, which seriously limit the possibility of redo surgery. The authors report successful surgical treatment of recurrent malignant inorganic retroperitoneal myxolipoma with giant baseline dimensions.
Subject(s)
Neoplasm Recurrence, Local , Retroperitoneal Neoplasms , Humans , Neoplasm Recurrence, Local/diagnosis , Neoplasm Recurrence, Local/surgery , Retroperitoneal Neoplasms/diagnosis , Retroperitoneal Neoplasms/surgery , Retrospective StudiesABSTRACT
A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.
Subject(s)
Breast Neoplasms/blood , CD24 Antigen/blood , Colorectal Neoplasms/blood , Exosomes/chemistry , Tetraspanin 28/blood , Tetraspanin 29/blood , Biomarkers/blood , Breast Neoplasms/pathology , CD24 Antigen/genetics , Case-Control Studies , Colorectal Neoplasms/pathology , Female , Gene Expression , Humans , Male , Middle Aged , Tetraspanin 28/genetics , Tetraspanin 29/geneticsABSTRACT
Activity of the proteasome, polyfunctional enzymatic complex, is known to undergo changes during cancer development. This phenomenon is, probably, caused by the changes in subunit composition of proteasomes. In present work, we studied chymotrypsin-like activity of proteasomes, subunit composition and their association in breast cancer, head and neck squamous cell carcinoma, endometrial cancer, renal cancer, bladder cancer, stomach cancer and colorectal cancer. The increase of proteasome activity was revealed in most cancer tissues compared with adjacent tissues except for the renal cell carcinoma. Changes in proteasome activity in cancer tissues compared with correspondent normal tissues were accompanied by modification of its subunit composition. High proteasome activity was observed in combination with an increased expression of immune subunits and/or proteasome activator PA28, associated with activity of 20S proteasome. In breast cancer, head and neck squamous cell carcinoma, bladder cancer, stomach cancer and colorectal cancer we additionally found higher expression of Rpt6 subunit of 26S proteasome. Correlations between chymotrypsin like proteasome activity and subunit expressions were found in human cancer tissues. In summary, we suggest that proteasome ac- tivation and changes in its subunit composition plays an important role in cancer pathogenesis.
