1.
Nat Methods
; 9(11): 1077-80, 2012 Nov.
Article
in English
| MEDLINE
| ID: mdl-23042452
ABSTRACT
We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.