ABSTRACT
O objetivo desta pesquisa foi identificar a ocorrência dos patógenos causadores de mastite subclínica em um rebanho leiteiro tipo B no município de Jaguapitã, estado do Paraná, Brasil. Foram realizados 400 testes de Califórnia Mastite Teste (CMT) em amostras de leite de 100 animais, totalizando 400 tetos. Dentre os animais testados 55% reagiram ao CMT apresentando grau dois ou superior, com 157 tetos positivos. Após as amostras de leite dos 157 tetos serem submetidos à cultura em ágar sangue, 25,48% (40/157) não apresentaram crescimento ou houve crescimento de mais de duas colônias bacterianas, 28,03% (44/157) foram observadas Staphylococcus coagulase negativa (CNS), 8,28% (13/157) Streptococcus uberis, 7,64% (12/157) Staphylococcus aureus, 7,64% (12/157) Corynebacterium spp, 7,01% (11/157) Staphylococcus intermedius, 4,46% (7/157) Staphylococcus hyicus, 3,82% (6/157) Bacillus spp., 2,55% (4/157) para Streptococcus dysgalacteae, Enterobactéria e Leveduras. Conclui-se que a CNS é o mais relevante agente causador de mastite subclínica.(AU)
The aim of this research was to identify the occurrence of pathogens causing subclinical mastitis in grade B milk farms of the Jaguapitã county, state of Paraná, Brazil. California Mastitis Test (CMT) were carried out in 400 milk samples from 100 animals and 157 teats from 55 animals (55%) were positive, showed score two or higher to CMT. When these 157 positive samples to CMT were transported for bacterial culture in blood agar, 25.48% (40/157) samples showed no bacterial growth or more than two types of bacterial colonies grew, 28.03% (44/157) were Coagulase-negative staphylococci (CNS), 8.28% (13/157) were Streptococcus uberis, 7.64% (12/157) were Staphylococcus aureus, 7.64% (12/157) were Corynebacterium spp, 7.01% (11/157) were Staphylococcus intermedius, 4.46% (7/157) were Staphylococcus hyicus, 3.82% (6/157) were Bacillus spp., 2.55% (4/157) were Streptococcus dysgalacteae, Enterobacteria and Yeasts. We conclude that CNS is the most relevant subclinical mastitis causative agent.(AU)
Subject(s)
Animals , Cattle , Mastitis/veterinary , Mastitis, Bovine/epidemiology , Milk/microbiology , Staphylococcus , CorynebacteriumABSTRACT
O objetivo desta pesquisa foi identificar a ocorrência dos patógenos causadores de mastite subclínica em um rebanho leiteiro tipo B no município de Jaguapitã, estado do Paraná, Brasil. Foram realizados 400 testes de Califórnia Mastite Teste (CMT) em amostras de leite de 100 animais, totalizando 400 tetos. Dentre os animais testados 55% reagiram ao CMT apresentando grau dois ou superior, com 157 tetos positivos. Após as amostras de leite dos 157 tetos serem submetidos à cultura em ágar sangue, 25,48% (40/157) não apresentaram crescimento ou houve crescimento de mais de duas colônias bacterianas, 28,03% (44/157) foram observadas Staphylococcus coagulase negativa (CNS), 8,28% (13/157) Streptococcus uberis, 7,64% (12/157) Staphylococcus aureus, 7,64% (12/157) Corynebacterium spp, 7,01% (11/157) Staphylococcus intermedius, 4,46% (7/157) Staphylococcus hyicus, 3,82% (6/157) Bacillus spp., 2,55% (4/157) para Streptococcus dysgalacteae, Enterobactéria e Leveduras. Conclui-se que a CNS é o mais relevante agente causador de mastite subclínica.
The aim of this research was to identify the occurrence of pathogens causing subclinical mastitis in grade B milk farms of the Jaguapitã county, state of Paraná, Brazil. California Mastitis Test (CMT) were carried out in 400 milk samples from 100 animals and 157 teats from 55 animals (55%) were positive, showed score two or higher to CMT. When these 157 positive samples to CMT were transported for bacterial culture in blood agar, 25.48% (40/157) samples showed no bacterial growth or more than two types of bacterial colonies grew, 28.03% (44/157) were Coagulase-negative staphylococci (CNS), 8.28% (13/157) were Streptococcus uberis, 7.64% (12/157) were Staphylococcus aureus, 7.64% (12/157) were Corynebacterium spp, 7.01% (11/157) were Staphylococcus intermedius, 4.46% (7/157) were Staphylococcus hyicus, 3.82% (6/157) were Bacillus spp., 2.55% (4/157) were Streptococcus dysgalacteae, Enterobacteria and Yeasts. We conclude that CNS is the most relevant subclinical mastitis causative agent.