ABSTRACT
OBJECTIVE: To compare genotyping of leucocyte DNA by PCR and Dde I with the conventional genotyping of haemoglobin of the same subject. DESIGN: Comparative study. SUBJECTS: 25 adults, 16 males and nine females. MAIN OUTCOME MEASURES: Predictability. RESULTS: In all cases the results were comparable. CONCLUSIONS: The technology can be applied locally to prenatal diagnosis of sickle-cell disease by chorionic sampling and, therefore, improve the management of sickle-cell disease in Nigeria.
Subject(s)
Anemia, Sickle Cell/diagnosis , Anemia, Sickle Cell/genetics , DNA/analysis , Polymerase Chain Reaction/methods , Restriction Mapping/methods , Adult , Case-Control Studies , Chorionic Villi Sampling , Electrophoresis, Cellulose Acetate , Female , Genotype , Humans , Leukocytes , Male , Nigeria , Reproducibility of ResultsSubject(s)
Alkaline Phosphatase/blood , Child Development , Adolescent , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , NigeriaSubject(s)
Isoniazid/metabolism , Phenotype , Acetylation , Adult , Black People , Female , Humans , Isoniazid/therapeutic use , Male , Nigeria , Time Factors , Tuberculosis, Pulmonary/drug therapyABSTRACT
The ventilatory response to carbon dioxide was measured by a rebreathing technique in 21 Nigerians who had homozygous sickle-cell disease. The slope of the carbon dioxide response curve was obtained by plotting the ventilation at successive half-minute intervals against the corresponding mean end-tidal PCO2. Our results showed that sickle-cell patients are as sensitive to carbon dioxide as are normal subjects.
Subject(s)
Anemia, Sickle Cell/physiopathology , Carbon Dioxide , Respiration , Adolescent , Adult , Carbon Dioxide/analysis , Child , Female , Humans , MaleABSTRACT
Aerosol beclomethasone dipropionate was effective in maintaining airway patency in 14 out of 16 steroid-dependent asthmatics. In 3 patients who had significant adrenal suppression on systemic corticosteroid therapy substitution with aerosol beclomethasone led to recovery of adrenal function. From the evidence obtained it is concluded that this aerosol steroid represents a modest advance in the therapy of asthma.
Subject(s)
Anti-Asthmatic Agents/administration & dosage , Asthma/drug therapy , Beclomethasone/administration & dosage , Administration, Inhalation , Adult , Female , Glucocorticoids , Humans , Hydrocortisone/blood , Male , Middle AgedABSTRACT
Oxygen consumption (VO2) was determined in 10 patients with moderate tetanus. The mean (+/- SE) VO2 of 425-2-2 +/- 50-3 ml/min on admission fell significantly to 249-9 +/- 13-1 ml/min standard temperature and pressure dry (STPD) after parenteral diazepam. There was also a significant fall in minute ventilation after administration of diazepam. The results suggest that the simple practical determination of VO2 will be of immense value in assessing the efficacy of muscle relaxants, assessing the severity of tetanus, and determining the calorie needs of patients.
Subject(s)
Oxygen Consumption , Tetanus/drug therapy , Adult , Aged , Diazepam/therapeutic use , Drug Evaluation/methods , Female , Humans , Male , Oxygen Consumption/drug effects , Respiration/drug effects , Tetanus/metabolismABSTRACT
Ventilatory response to carbon dioxide was measured by the rebreathing technique in 32 Nigerians and 20 Europeans resident in Lagos. Although the Europeans had larger physical characteristics and lung volumes than Nigerians, the responses to carbon dioxide were similar. The range of ventilatory response was 0,55 to 4,2 and 0,7 to 3,7 1/min/mm Hg for Nigerians and Europeans respectively. These results show that Nigerians are as sensitive to carbon dioxide as their European counterparts.
Subject(s)
Black People , Carbon Dioxide , Respiration , Adult , Carbon Dioxide/blood , Europe/ethnology , Female , Forced Expiratory Volume , Humans , Male , Nigeria , Partial Pressure , Vital Capacity , White PeopleABSTRACT
Patterns of response to carbon dioxide (CO2) inhalation in 12 Nigerian asthmatic patients before and after salbutamol aerosol inhalation are described. Ventilatory response to CO2 inhalation follows three patterns, viz,(1) an increase; (2) a decrease; (3) no change in response. While most of our subjects showed substantial improvement in forced expiratory volume in one second (FEV1) following bronchodialator therapy, there was no corresponding change in ventilatory response to CO2. On the whole. the mean values of the slope of response to CO2 inhalation of the asthmatics before and after bronchodilator were not significantly different from values obtained in normal patients in our laboratory.
