Mycorrhizal symbiosis alleviate salinity stress in pistachio plants by altering gene expression and antioxidant pathways.

This study investigated how inoculation of salt-stressed Pistacia vera seedlings with Rhizophagus irregularis, an arbuscular mycorrhizal fungus (AMF), affects their biomass, oxidative damage, antioxidant enzyme activity, and gene expression. Pistachio seedlings (N:36) were randomly assigned to AMF inoculation and non-inoculation groups in a pot experiment with 9 replications. Each group was further divided and randomly assigned to two salinity treatments (0 and 300 mM NaCl). At the end of week 4, three pistachio plantlets were randomly selected from each group for Rhizophagus irregularis colonization inspection, physiological and biochemical assays, and biomass measurements. Salinity activated enzymatic and non-enzymatic antioxidant systems in the pistachio plants were studied. The negative effects of salinity included reduced biomass and relative water content (RWC), increased O2 ·-, H2O2, MDA, and electrolytic leakage. Generally, Rhizophagus irregularis was found to mitigate the adverse effects of salinity in pistachio seedlings. AMF inoculation resulted in even further increases in the activities of SODs, POD, CAT, and GR enzymes, upregulating Cu/Zn-SOD, Fe-SOD, Mn-SOD, and GR genes expression in plants under salinity stress. Moreover, AMF significantly increased AsA, α-tocopherol, and carotenoids under both control and salinity conditions. The study concludes with a call for future research into the mechanisms of mycorrhiza-induced tolerance in plants under salinity stress. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01279-8.

Growth inhibition of human breast cancer cells and down-regulation of ODC1 and ADA genes by Nepeta binaloudensis

ABSTRACT Nepeta binaloudensis Jamzad, Lamiaceae, is a rare medicinal plant endemic to Iran. In spite of many studies about the chemical constituents and antibacterial effects of this species, no report has been provided about its cytotoxic and anticancer activities. In this study we have evaluated the effects of EtOH 70%, hexane and aqueous extracts of N. binaloudensis on the cell proliferation and n-hexane extract on the expression of adenosine deaminase and ornithine decarboxylase 1 genes in breast cancer cell lines (MCF-7, MDA-MB-231) compared to non-cancer line (MCF-10A). The cell lines were subjected to increasing doses of the extracts ranging from 10 to 320 µg/ml. Cell viability was quantified by MTS assay. Expression of adenosine deaminase and ornithine decarboxylase 1 genes was analyzed by real time PCR. N. binaloudensis inhibited the growth of malignant cells in a time and dose-dependent manner. Among extracts of N. binaloudensis, the hexane extract was found to be more toxic compared to other extracts. Results showed a marked decrease in the expression of ornithine decarboxylase 1 and adenosine deaminase genes in cancer cell lines. At 60 µg/ml concentration of N. binaloudensis hexane extract ornithine decarboxylase 1 and adenosine deaminase mRNA expression were reduced 4.9 fold and 3.5 fold in MCF-7 cell line and 3.6 fold and 2.6 fold in MDA-MB-231 cell line compared to control, respectively. The result of our study highlights the potential influences of N. binaloudensis hexane extract on ornithine decarboxylase 1 and adenosine deaminase genes expression in breast cancer cells and its relation to inhibition of cancer cell growth.