ABSTRACT
The goal of the present study was to evaluate the encapsulation of Weissella paramesenteroides, isolated from bee bread, as a technological tool for its use in biological fish silage. The pH decrease in fish silages using the bacteria encapsulated and in a non-encapsulated form was compared. W. paramesenteroides showed a good performance in the development of biological fish silage. The alginate encapsulation method showed an encapsulation efficacy of 85% and provides a reliable technological application.
Subject(s)
Fishes , Food Technology/methods , Silage , Weissella , AnimalsABSTRACT
The goal of the present study was to evaluate the encapsulation of Weissella paramesenteroides, isolated from bee bread, as a technological tool for its use in biological fish silage. The pH decrease in fish silages using the bacteria encapsulated and in a non-encapsulated form was compared. W. paramesenteroides showed a good performance in the development of bioEnc ap su lati on; logical fish silage. The alginate encapsulation method showed an encapsulation efficacy of 85% and provides a reliable technological application.
El objetivo del presente estudio fue evaluar la encapsulación de Weissella paramesenteroides, aislada a partir del pan de polen, como herramienta tecnológica para su uso en la elaboración de ensilado biológico de pescado. Se comparó el descenso de pH para los ensilados utilizando la bacteria encapsulada y no encapsulada. W. paramesenteroides mostró un buen desempeño en el desarrollo de ensilado biológico de pescado. El método de encapsulación con alginato mostró una eficacia del 85% y puede ser utilizado para su aplicación tecnológica.
Subject(s)
Silage/microbiology , Leuconostocaceae/isolation & purification , Fish Products/analysis , Waste ProductsABSTRACT
Storage in ice is a common way of preserving commercial fish species but some microorganisms can still contaminate and participate in the spoilage of the product; therefore, identification of potential harmful microbes is important. Thirteen colonies were isolated from common carp (Cyprinus carpio) that had been stored in ice, whose phenotypic identification revealed that they belonged to the genera Aeromonas (n = 5) and Shewanella (n = 8). Molecular genotyping with ERIC-PCR showed clonality only among two of the five Aeromonas isolates and for two groups (n = 3; n = 2) of the eight Shewanella isolates. Sequencing the rpoD gene showed that four Aeromonas isolates belonged to the species Aeromonas salmonicida and one to A. sobria. Of the eight Shewanella, seven isolates cluster with Shewanella putrefaciens and one with Shewanella profunda in the 16S rRNA phylogenetic tree. However, analysis of the gyrB gene showed that these eight isolates could constitute a new species closely related to S. baltica. The Shewanella and A. salmonicida isolates produce off-odours and reduce trimethylamine oxide, indicating that they might contribute to the spoilage of the fish.
