ABSTRACT
Activities of some enzymes and content of medium-weight molecules in patients with colorectal cancer were studied in order to assess the diagnostic value of these parameters for detection of tumors in the large intestine and development of endogenous intoxication after surgery and for prediction of remote results of treatment. Increased activities of creatine phosphokinase and hexokinase is typical of tumor growth, whereas increased activities of alkaline phosphatase and gamma-glutamyl transpeptidase are observed only in metastases to the liver. The role of medium-weight molecules in the diagnosis of endogenous intoxication during the early postoperative period is shown. The content of these molecules in the sera increases 3 days before clinical manifestation of endotoxicosis, when the traditional parameters are virtually normal.
Subject(s)
Adenocarcinoma/enzymology , Biomarkers, Tumor , Colorectal Neoplasms/enzymology , Creatine Kinase/blood , Adenocarcinoma/pathology , Adenocarcinoma/physiopathology , Colorectal Neoplasms/blood , Colorectal Neoplasms/pathology , Colorectal Neoplasms/physiopathology , Humans , Isoenzymes/blood , Neoplasm Metastasis , PrognosisSubject(s)
Anti-Bacterial Agents/administration & dosage , Cyanoacrylates/administration & dosage , Disease Models, Animal , Drainage/instrumentation , Peritonitis/surgery , Tissue Adhesives/administration & dosage , Animals , Combined Modality Therapy , Drainage/methods , Drug Implants , Peritonitis/etiology , Postoperative Care , RatsABSTRACT
The blood levels of cyclic nucleotides and serotonin were measured in 143 patients with functional and inflammatory diseases of the large intestine. The patients were divided into 3 groups administered various treatment: drug (basic) therapy in Group 1, basic therapy + alternating magnetic field in Group 2, and basic therapy + sinusoidal modulated currents in Group 3. Follow-up of the examined parameters over the course of treatment has shown that by day 16 of inpatient treatment they normalize, this demonstrating the treatment efficacy.
Subject(s)
Colitis/blood , Colonic Diseases, Functional/blood , Cyclic AMP/blood , Cyclic GMP/blood , Serotonin/blood , Colitis, Ulcerative/blood , Fluorometry , Humans , RadioimmunoassayABSTRACT
On the corazole model of the experimental epilepsy and epileptiform activity evoked by penicillin application to the cerebral cortex with electroencephalographic registration, it has been shown that exogenous DNAs in the most cases completely or partially inhibit convulsive activity. DNA inhibited also the pacemaker activity of the Helix RPa-1 neurons.
Subject(s)
DNA/therapeutic use , Seizures/drug therapy , Animals , DNA/pharmacology , Drug Evaluation, Preclinical , Electroencephalography , Epilepsy/chemically induced , Epilepsy/drug therapy , Helix, Snails , Humans , Neurons/drug effects , Penicillins , Pentylenetetrazole , Rats , Seizures/chemically induced , Time FactorsABSTRACT
Ca-dsRNA introduced in the concentration of 5 micrograms per mouse 24 hours before the MG22a mouse hepatoma transplantation causes the 70% inhibition of the hepatoma growth. The sodium nucleinate used in the considerably higher concentration had a less effect. The sodium form of dsRNA also had a less antitumour effect comparing with its calcium form. The calcium form of dsRNA proves to be relatively stable and physiologically active form of dsRNA during its parenteral administration.
Subject(s)
Calcium/therapeutic use , Liver Neoplasms, Experimental/drug therapy , Nucleic Acids/therapeutic use , RNA, Double-Stranded/therapeutic use , RNA, Fungal/therapeutic use , Animals , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Mice , Mice, Inbred C3H , Neoplasm TransplantationABSTRACT
Plasmid DNAs were isolated from Bacillus thuringiensis and their physico-chemical properties were studied. The DNAs were purified using ultracentrifugation in a CsCl2 density gradient. The molecular weight of these extrachromosomal DNAs determined by electrophoresis in 0.6% agarose gel and by electron microscopy was 6 . 10(6)--10 . 10(6) daltons; a DNA with a molecular weight of 90 . 10(6) daltons was also found. Digestion with restriction endonuclease EcoRI yielded fragments of the plasmid DNAs with cohesive ends; these were further identified by means of electrophoresis in 1% agarose gel.
Subject(s)
Bacillus thuringiensis/analysis , DNA, Bacterial/isolation & purification , Plasmids , Chemical Phenomena , Chemistry, Physical , DNA, Bacterial/analysis , Electrophoresis, Agar Gel/methods , Molecular Weight , Ultracentrifugation/methodsABSTRACT
Physico-chemical characteristics of plasmid DNAs isoalted from Pseudomonas putida G7 were studied as well as the behavior of these DNAs in th eourse of chromatography on columns with Sepharose 4B and kieselguhr with methylated albumin (MAC). This strain was found to contain several plasmid DNAs having molecular weights of 33-36X10(6), 15-18X10(6), and 3-5X10(6) dalton. The plasmid DNAs of biodegradation are supposed to be located in the vicinity of chromosomes, and only a small part of them is characterized by extrachromosomal localization.
